[1]任可可,秦明春.寿胎丸对人早孕滋养细胞增殖与凋亡的影响及相关机制研究[J].西部中医药,2026,39(03):1-5.[doi:10.12174/j.issn.2096-9600.2026.03.01]
　REN Keke,QIN Mingchun.Effect of Shoutai Pill on Proliferation and Apoptosis of Human Trophoblast Cells in Early Pregnancy and Its Related Mechanism[J].Western Journal of Traditional Chinese Medicine,2026,39(03):1-5.[doi:10.12174/j.issn.2096-9600.2026.03.01]
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寿胎丸对人早孕滋养细胞增殖与凋亡的影响及相关机制研究()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
1-5
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Effect of Shoutai Pill on Proliferation and Apoptosis of Human Trophoblast Cells in Early Pregnancy and Its Related Mechanism
作者:
任可可1, 秦明春2
1.广西中医药大学，广西 南宁 530001
2.广西中医药大学附属瑞康医院，广西 南宁 530011
Author(s):
REN Keke1, QIN Mingchun2
1.Guangxi University of Chinese Medicine, Nanning 530001, China
2.Ruikang Hospital affiliated to guangxi university of chinese medicine,Nanning, 530011, China
关键词:
人早孕滋养细胞; 寿胎丸; 增殖; 凋亡; 增殖细胞核抗原; 趋化因子C-X-C基序配体12; 趋化因子C-X-C基序配体16
Keywords:
Human early pregnancy trophoblast; pill; proliferation; apoptosis; CXCL12; CXCL16
分类号:
R271.41
DOI:
10.12174/j.issn.2096-9600.2026.03.01
文献标志码:
A
摘要:
目的探讨寿胎丸含药血清对人早孕滋养细胞增殖、增殖细胞核抗原（（proliferating cellnuclear antigen，PCNA）表达、迁移、凋亡及趋化因子C-X-C基序配体12（C-X-C motif chemokine ligand 12，CXCL12）、C-X-C基序配体16（C-X-C motif chemokine ligand 16，CXCL16）分泌的影响。 方法体外分离培养人早孕滋养细胞，设置阴性血清组（对照组）、地屈孕酮含药血清组（阳性对照组）及寿胎丸含药血清低、中、高剂量组（干预组）。采用CCK-8法检测细胞增殖活力，流式细胞术检测PCNA表达及细胞凋亡率，Transwell实验检测细胞迁移能力，ELISA法检测细胞上清液中CXCL12、CXCL16的含量。 结果与阴性血清组相比，寿胎丸各剂量含药血清组人早孕滋养细胞增殖活性、PCNA表达率及迁移能力均增强，细胞凋亡率下降，CXCL12、CXCL16含量升高（P＜0.05）；与寿胎丸低剂量组相比，中、高剂量组上述效应更明显（P＜0.05），且呈浓度依赖性（P＜0.05）。 结论寿胎丸含药血清可通过上调CXCL12、CXCL16的表达，提高人早孕滋养细胞的增殖活性及迁移能力，降低细胞凋亡率，从而发挥维持正常妊娠的作用。
Abstract:
ObjectiveTo investigate the effects of the serum containing Shoutai pill on the proliferation,PCNA expression, migration and apoptosis of human trophoblasts in early pregnancy, and the secretion of the chemokines CXCL12 and CXCL16. MethodsThe human trophoblasts of early pregnancy were isolated and cultured in vitro,and were intervened with low,medium and high doses of serum containing Shoutai Pill,with negative serum group (the control group)and desogestrel group as positive control groups respectively. CCK-8, flow cytometry, Transwell,AnnexinV-FITC/PI double labeled flow cytometry and ELISA were used to determine the effects of serum containing drugs on the proliferation activity,PCNA expression,migration ability,total apoptosis,chemokines CXCL12 and CXCL16 of human trophoblasts in early pregnancy. ResultsCompared with the negative group,after the intervention of the serum containing Shoutai Pill,the proliferation activity,invasion and migration ability of human trophoblasts in early pregnancy were significantly enhanced,the apoptosis rate was significantly decreased,and the contents of chemokines CXCL12 and CXCL16 were significantly increased (P<0.05).Compared with the low-dose group of Shoutai Pill,the proliferation activity,invasion and migration ability of trophoblast cells in the middle and high-dose groups of Shoutai Pill were significantly enhanced,and the contents of chemokines CXCL12 and CXCL16 were significantly increased (P<0.05). ConclusionThe medicated serum of Shoutai Pill can improve the proliferation activity,invasion and migration ability of human trophoblasts in early pregnancy,and the content of chemokines CXCL12 and CXCL16,reduce the apoptosis rate and promote cell proliferation.

相似文献/References:

[1]周静,陈萍.寿胎丸联合阿司匹林及泼尼松治疗复发性流产41例[J].西部中医药,2018,31(06):88.
　ZHOU Jing,CHEN Ping.ShouTai Pills, Aspirin and Prednison in the Treatment for 41 Cases of Recurrent Spontaneous Abortion[J].Western Journal of Traditional Chinese Medicine,2018,31(03):88.

备注/Memo

备注/Memo:
广西自然科学基金（2018JJA140544）；广西中医药大学2021年研究生教育创新计划项目（YCXJ2021087）；广西中医药大学2020年校级教育教学改革与研究重点项目（2020A024）。任可可（1990—），女，硕士学位，主治医师。研究方向：中医药对生殖内分泌与妊娠的免疫调节。
更新日期/Last Update: 2026-03-15