[1]贾世艳,仲启明,司瑞花,等.复方蛇龙胶囊对膜性肾病大鼠肾保护作用及对足细胞凋亡的影响[J].西部中医药,2026,39(03):6-11.[doi:10.12174/j.issn.2096-9600.2026.03.02]
　JIA Shiyan,ZHONG Qiming,SI Ruihua,et al.Renal Protection and Podocyte Apoptosis Modulation by Compound Shelong Capsule in Rats with Membranous Nephropathy[J].Western Journal of Traditional Chinese Medicine,2026,39(03):6-11.[doi:10.12174/j.issn.2096-9600.2026.03.02]
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复方蛇龙胶囊对膜性肾病大鼠肾保护作用及对足细胞凋亡的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
6-11
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Renal Protection and Podocyte Apoptosis Modulation by Compound Shelong Capsule in Rats with Membranous Nephropathy
作者:
贾世艳1,2,3, 仲启明3, 司瑞花2,3, 范晓阳3, 严文允2, 刘光珍3
1.河北省沧州中西医结合医院，河北 沧州 061000
2.山西中医药大学，山西 晋中 030619
3.山西省中医药研究院，山西 太原 030012
Author(s):
JIA Shiyan1,2,3, ZHONG Qiming3, SI Ruihua2,3, FAN Xiaoyang3, YAN Wenyun2, LIU Guangzhen3
1.Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine in Hebei Province, Cangzhou 061000, China
2.Shanxi University of Traditional Chinese Medicine, Jinzhong 030619, China
3.Shanxi Academy of Traditional Chinese Medicine, Taiyuan 030012, China
关键词:
膜性肾病; 复方蛇龙胶囊; 大鼠; 足细胞; 凋亡
Keywords:
membranous nephropathy; compound capsule; rats; podocyte; apoptosis
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2026.03.02
文献标志码:
A
摘要:
目的研究复方蛇龙胶囊对阳离子化牛血清白蛋白诱导的膜性肾病大鼠肾保护作用及对足细胞凋亡的影响。 方法60只6周龄SPF级雄性SD大鼠，随机选取10只作为正常组，其余均采用阳离子化牛血清白蛋白（cationic bovine serum albumin，C-BSA）诱导构建膜性肾病大鼠模型，模型制备成功后随机分为模型组、雷公藤多苷片组（3 mg·kg-1）和复方蛇龙胶囊组（1.5 g·kg-1）。正常组和模型组灌胃给予等体积的生理盐水，每日1次，连续干预4周。采用全自动生化分析仪检测血清中肌酐（serum creatinine，SCr）、甘油三酯（triglycerides，TG）、总胆固醇（total cholesterol，TC）、白蛋白（albumin，ALB）、总蛋白（total protein，TP）含量；酶联免疫吸附试验检测尿微量白蛋白（urinary microalbumin，U-mALB）含量；苏木素-伊红（hematoxylin-eosin，HE）染色、马松（Masson）染色、透射电镜和扫描电镜观察肾组织病理学变化；流式细胞术检测肾组织细胞凋亡率；实时荧光定量聚合酶链反应（real-time quantitative polymerase chain reaction，RT-qPCR）检测肾组织肾小球足细胞滤过裂孔蛋白（nephrin）、足细胞鞘膜蛋白（podocin）、B细胞淋巴瘤2（B-cell lymphoma 2，Bcl-2）、Bcl-2相关X蛋白（bcl-2-associated x protein，Bax）和半胱天冬酶3（cysteine-aspartic protease 3，Caspase-3）mRNA表达水平。 结果与正常组比较，模型组大鼠血清中ALB和TP明显降低（P＜0.05），TC、TG、SCr和U-mALB明显升高（P＜0.05）；光镜下可见肾小球结构紊乱，炎性细胞浸润，纤维组织沉积；电镜下可见肾小球基底膜增厚，足突融合，足细胞损伤；肾组织细胞凋亡率明显升高（P＜0.05）；nephrin和podocin mRNA表达水平明显下降（P＜0.05）；Bcl-2 mRNA表达水平明显降低，Bax和Caspase-3 mRNA表达水平明显升高，Bcl-2/Bax比值明显降低（P＜0.05）。与模型组比较，各给药组大鼠血清中ALB和TP明显升高，TC、TG、SCr和U-mALB明显降低（P＜0.05）；肾组织病理损伤明显改善；肾组织细胞凋亡率明显降低（P＜0.05）；Nephrin和Podocin mRNA表达水平明显升高（P＜0.05）；Bcl-2 mRNA表达水平显著升高，Bax和Caspase-3 mRNA表达水平明显降低，Bcl-2/Bax比值明显升高（P＜0.05）。 结论复方蛇龙胶囊对C-BSA诱导的膜性肾病大鼠肾损伤具有保护作用，其机制可能与抑制足细胞凋亡有关。
Abstract:
ObjectiveTo investigate the renal protective effect of compound Shelong capsule on membranous nephropathy induced by cationic bovine serum albumin in rats and its impact on podocyte apoptosis. MethodsSixty 6-week-old SPF-grade male Sprague-Dawley rats were used in this study. Ten rats were randomly selected as the normal control group, while the remaining rats were subjected to induction of membranous nephropathy using cationic bovine serum albumin. Following successful model establishment, the model rats were randomly divided into three groups: the model group, the tripterygium glycosides tablet group (3 mg·kg??), and compound Shelong capsule group (1.5 g·kg??). The normal control and model groups were administered an equal volume of normal saline intragastrically once daily for four consecutive weeks. Serum levels of creatinine (SCr), triglycerides (TG), total cholesterol (TC), albumin (ALB), and total protein (TP) were measured using an automatic biochemical analyzer. Urinary microalbumin (U-mALB) levels were detected by enzyme-linked immunosorbent assay (ELISA). Renal tissue pathological changes were observed using hematoxylin-eosin (HE) staining, Masson staining, transmission electron microscopy, and scanning electron microscopy. The apoptosis rate of renal cells was detected by flow cytometry. The mRNA expression levels of nephrin, podocin, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and cysteine-aspartic protease 3 (Caspase-3) in renal tissue were detected by real-time quantitative polymerase chain reaction (RT-qPCR). ResultsCompared with the normal control group, serum levels of ALB and TP were significantly decreased in the model group (P<0.05), while TC, TG, SCr, and U-mALB were significantly increased (P<0.05). Light microscopy revealed glomerular structural disorganization, inflammatory cell infiltration, and fibrous tissue deposition in the model group. Electron microscopy showed thickening of the glomerular basement membrane, foot process fusion, and podocyte injury. The apoptosis rate of renal cells was significantly elevated in the model group (P<0.05). The mRNA expression levels of nephrin and podocin were significantly downregulated (P<0.05). Additionally, Bcl-2 mRNA expression was significantly decreased, while Bax and Caspase-3 mRNA expression were significantly increased, leading to a marked reduction in the Bcl-2/Bax ratio (P<0.05). Compared with the model group, rats in the treatment groups exhibited significantly increased serum levels of ALB and TP, along with significantly decreased levels of TC, TG, SCr, and U-mALB (P<0.05). Pathological damage to renal tissue was notably ameliorated. The apoptosis rate of renal cells was significantly reduced (P<0.05). The mRNA expression levels of nephrin and podocin were significantly upregulated (P<0.05). Furthermore, Bcl-2 mRNA expression was significantly increased, whereas Bax and Caspase-3 mRNA expression were significantly decreased, resulting in a significantly elevated Bcl-2/Bax ratio (P<0.05). ConclusionCompound Shelong capsule exerts a protective effect against renal injury in rats with C-BSA-induced membranous nephropathy, and its mechanism may be associated with the inhibition of podocyte apoptosis.

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备注/Memo

备注/Memo:
山西省重点研发计划重点项目（201603D3113023，201703D421024）；山西省科技攻关项目（2016ZD0307）；山西省晋药综合开发利用协同创新中心项目（2017-JYXT-32）。贾世艳（1994—），女，硕士学位。研究方向：实验方剂学及方剂药理学研究。
更新日期/Last Update: 2026-03-15