[1]刘军洋,滕红丽,赵金妹,等.两种细叶勾儿茶单体化合物的抗炎活性相关研究[J].西部中医药,2026,39(03):12-17.[doi:10.12174/j.issn.2096-9600.2026.03.03]
　LIU Junyang,TENG Hongli,ZHAO Jinmei,et al.Studies on Anti-inflammatory Activities of Two Kinds of Monomer Compounds of Berchemia Lineata[J].Western Journal of Traditional Chinese Medicine,2026,39(03):12-17.[doi:10.12174/j.issn.2096-9600.2026.03.03]
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两种细叶勾儿茶单体化合物的抗炎活性相关研究()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
12-17
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Studies on Anti-inflammatory Activities of Two Kinds of Monomer Compounds of Berchemia Lineata
作者:
刘军洋1, 滕红丽2, 赵金妹2, 洪精2
1.广西中医药大学，广西 南宁 530001
2.广西中医药大学附属国际壮医医院，广西 南宁 530201
Author(s):
LIU Junyang1, TENG Hongli2, ZHAO Jinmei2, HONG Jing2
1.Guangxi University of Chinese Medicine, Nanning 530001, China
2.Guangxi International Zhuang Medicine Hospital, Nanning 530201, China
关键词:
细叶勾儿茶; 单体化合物; 抗炎
Keywords:
berchemia lineata; monomeric compound; anti-inflammation
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2026.03.03
文献标志码:
A
摘要:
目的对细叶勾儿茶分离提取的01号萘骈α-吡喃酮类单体化合物和02号色原酮类单体化合物进行抗炎活性筛选，并探究其抗炎机制。 方法向小鼠RAW264.7巨噬细胞中加入0.5 μg/mL脂多糖（lipopolysaccharide，LPS），经LPS胞外刺激12 h，激活细胞产生炎症反应；CCK-8法检测浓度为50、75 μM时01、02号单体化合物对RAW264.7细胞存活率的影响；对01、02号单体化合物设置浓度梯度（0、6.25、12.5、25、50、75 μM），通过Griess法检测各组RAW264.7细胞上清液中NO的含量并计算NO抑制率，对01、02号单体化合物进行抗炎活性梯度检测；通过ELISA法检测01号单体化合物与LPS共孵育12 h对肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、白细胞介素6（interleukin-6，IL-6）、核转录因子κB（nuclear factor-κB，NF-κB）的影响；通过ELISA法检测02号单体化合物与LPS共孵育12 h对IL-6、TNF-α、NF-κB、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）、环氧合酶2（cyclooxygenase 2，COX-2）的影响；通过PCR法检测01号单体化合物与LPS共孵育12 h对RAW264.7细胞IL-1β、IL-6以及iNOS mRNA表达的影响；通过PCR法检测02号色原酮类化合物对LPS诱导的RAW264.7细胞TNF-α、COX-2、iNOS mRNA的表达的影响。 结果从细叶勾儿茶中分离所得的两种化合物，在50、75 μΜ下对RAW264.7存活率无明显影响；对细胞上清液的NO含量检测结果显示，随着化合物浓度的增加，细胞上清液中NO含量明显减少；01号化合物可降低LPS诱导的RAW264.7细胞的细胞上清液中IL-6、TNF-α、NF-κB的表达水平，02号化合物可降低TNF-α、IL-6、NF-κB、iNOS、COX-2的表达水平。01号化合物可抑制LPS诱导的RAW264.7细胞IL-6、IL-1β、iNOS的mRNA表达水平；02号化合物可抑制TNF-α、iNOS、COX-2的mRNA表达水平。 结论从细叶勾儿茶中提取的两种化合物均无明显的药物毒性，具有一定的抗炎活性，并且随着化合物浓度增加，抗炎活性明显增加。两种单体化合物可能通过调控NF-κB信号通路发挥抗炎作用。
Abstract:
ObjectiveTo screen anti-inflammation of No 1 naphthyl-α-pyranone monomer compounds and No 2 chromenone monomer compounds separated and extracted from berchemia lineata, and to investigate its anti-inflammatory mechanism. Methods0.5 μg/mL lipopolysaccharide (LPS) was added to mouse macrophages RAW264.7 to active the cells and trigger the inflammatory response after LPS extracellular stimulus for 12 h; CCK-8 method was applied to detect the effects of monomer compounds No 1 and 2 on the survival rates of RAW264.7 cells when the concentrations were 50 and 75 μM; when concentration gradient (0, 6.25, 12.5, 25, 50 and 75 μM) was set for monomer compounds No 1 and 2, the contents of NO contained in RAW264.7 cellular supernatant were detected by Griess method, and NO inhibition rate was calculated, so as to measure anti-inflammatory activity gradient; ELISA method was adopted to measure the influence of monomer compounds No 1 after co-incubating with LPS for 12 on the levels of TNF-α, IL-6 and NF-κB; and to measure the effects of monomer compounds No 2 after co-incubating with LPS for 12 on the levels of IL-6, TNF-α, and NF-κB, iNOS and COX-2; PCR was used to detect the influence of monomer compounds No 1 after co-incubating with LPS for 12 on the expressions of IL-1β, IL-6 and iNOS mRNA in RAW264.7 cells; the effects of monomer compounds No 2 on the expressions of TNF-α, COX-2 and iNOS mRNA in LPS - induced RAW264.7 cells were measured via PCR method. ResultsTwo compounds separated from the herb showed no significant impacts on the survival rate of RAW264.7 cells at the concentrations of 50 and 75 μΜ; the detection results of the contents of NO contained in cellular supernatant displayed that the concentration of NO was reduced markedly as the concentrations of the compounds increased; monomer compounds No 1 could lower the levels of IL-6, TNF-α, and NF-κB in cellular supernatant of LPS - induced RAW264.7 cells, monomer compounds No 2 could reduce the expressions of TNF-α, IL-6, and NF-κB, iNOS and COX-2. Monomer compounds No 1 could inhibit the expressions of IL-6, IL-1β and iNOS mRNA in LPS - induced RAW264.7 cells; monomer compounds No 2 could restrain the expressions of TNF-α, iNOS and COX-2 mRNA. ConclusionTwo compounds extracted from the herb have no obvious drug toxicity, and certain anti-inflammatory activity, the anti-inflammatory activity was increased evidently with the increase of compound concentration. Two compounds could exert anti-inflammatory effects possibly through regulating NF-κB signaling pathway.

备注/Memo

备注/Memo:
国家自然科学基金（U25A20151）；国家重点研发计划项目（2022YFC3502201）。刘军洋（1994—），女，硕士学位。研究方向：民族医学理论及诊疗体系的研究。
更新日期/Last Update: 2026-03-15