[1]倪祥惠,吴菁,郭正阳,等.季节变化对CUMS模型动物肝脏BSEP基因mRNA表达的影响[J].西部中医药,2026,39(03):18-21.[doi:10.12174/j.issn.2096-9600.2026.03.04]
　NI Xianghui,WU Jing,GUO Zhengyang,et al.Influences of Seasonal Changes on BSEP Gene mRNA Expressions in the Liver of CUMS Animal Models[J].Western Journal of Traditional Chinese Medicine,2026,39(03):18-21.[doi:10.12174/j.issn.2096-9600.2026.03.04]
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季节变化对CUMS模型动物肝脏BSEP基因mRNA表达的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
18-21
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Influences of Seasonal Changes on BSEP Gene mRNA Expressions in the Liver of CUMS Animal Models
作者:
倪祥惠1, 吴菁2, 郭正阳3, 林川烘4
1.山东省第二人民医院/山东省耳鼻喉医院，山东 济南 250000
2.宁德市紧急救援中心，福建 宁德 352000
3.长葛市人民医院，河南 长葛 461500
4.万宁市中医院，海南 万宁 571500
Author(s):
NI Xianghui1, WU Jing2, GUO Zhengyang3, LIN Chuanhong4
1.Shandong Second Provincial General Hospital/Shandong Provincial ENT Hospital, Jinan 250000, China
2.Ningde Emergency Center, Ningde 352000, China
3.Changge People’s Hospital, Changge 461500, China
4.Wanning Hospital of TCM, Wanning 571500, China
关键词:
肝主疏泄; 慢性轻度不可预见性应激; 四时五脏阴阳; 胆酸盐输出泵基因
Keywords:
liver governs free coursing; CUMS; four seasons;  five viscera;  and; BSEP
分类号:
R223
DOI:
10.12174/j.issn.2096-9600.2026.03.04
文献标志码:
A
摘要:
目的观察季节变化对慢性轻度不可预见性应激（chronic unpredictable mild stress，CUMS）模型动物肝脏胆酸盐输出泵（bile salt export pump，BSEP）基因mRNA表达的影响。 方法选用健康雄性SD大鼠，分别于春分、夏至、秋分、冬至前1周购入，随机分为正常组与CUMS模型组；采用CUMS法构建肝失疏泄动物模型，通过实时荧光定量TaqMan检测肝脏组织中BSEP基因mRNA表达水平，分析其变化规律。 结果与同一季节正常组比较，CUMS组大鼠肝脏BSEP基因mRNA表达水平均显著增高；秋季各组大鼠肝脏BSEP基因mRNA表达水平均较春季组明显降低。 结论BSEP基因表达存在季节性变化特征，且该变化可能与肝主疏泄功能存在相关性。
Abstract:
ObjectiveTo observe the influences of seasonal changes on bile salt export pump (BSEP) gene mRNA expressions in the liver of CUMS animal models. MethodsHealthy male Sprague-Dawley (SD) rats were purchased one week before the vernal equinox, summer solstice, autumnal equinox, and winter solstice, respectively, and they were randomized into the normal group and CUMS model group. CUMS method was adopted to construct animal models of liver failing to govern free coursing, TaqMan real-time quantitative PCR was applied to detect the levels of BSEP gene mRNA in liver, and to analyze the trends. ResultsCompared with the normal group in the same season, CUMS group exhibits the elevated expressions of BSEP gene mRNA in liver; BSEP gene mRNA expressions in liver of the rats from all groups in autumn were lower than these in spring remarkably. ConclusionThe expressions of BSEP gene reveal seasonal changes, and the changes may be related to liver governing free coursing.

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备注/Memo

备注/Memo:
国家自然科学基金（81160418）。倪祥惠（1968—），男，硕士学位，硕士研究生导师，教授。研究方向：中医教学、科研与临床工作。Email：p1kmnxh@163.com。
更新日期/Last Update: 2026-03-15