[1]李文圃,侯亚威,王会浩,等.疏肝补肾方对被动型Heymann肾炎大鼠IL-6及TGF-β1/Smad信号通路的影响[J].西部中医药,2026,39(03):22-28.[doi:10.12174/j.issn.2096-9600.2026.03.05]
　LI Wenpu,HOU Yawei,WANG Huihao,et al.Effects of Liver-soothing Kidney-invigorating Prescription on IL-6 and TGF-β1/Smad Signaling Pathway in Rats with Passive Heymann Nephritis[J].Western Journal of Traditional Chinese Medicine,2026,39(03):22-28.[doi:10.12174/j.issn.2096-9600.2026.03.05]
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疏肝补肾方对被动型Heymann肾炎大鼠IL-6及TGF-β1/Smad信号通路的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
22-28
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Effects of Liver-soothing Kidney-invigorating Prescription on IL-6 and TGF-β1/Smad Signaling Pathway in Rats with Passive Heymann Nephritis
作者:
李文圃, 侯亚威, 王会浩, 肖振卫
山东中医药大学，山东 济南 250355
Author(s):
LI Wenpu, HOU Yawei, WANG Huihao, XIAO Zhenwei
Shandong University of Traditional Chinese Medicine, Jinan 250355, China
关键词:
被动型Heymann肾炎; 膜性肾病; 疏肝补肾方; 白细胞介素6; 信号通路
Keywords:
passive Heymann nephritis; membranous nephropathy; liver-soothing kidney-invigorating prescription; IL-6; signaling pathway
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2026.03.05
文献标志码:
A
摘要:
目的观察疏肝补肾方对被动型Heymann肾炎（passive Heymann nephritis，PHN）大鼠的治疗作用，探讨该方防治膜性肾病（membranous nephropathy，MN）的作用机制。 方法将32只雄性SD大鼠随机选取8只作为空白对照组，其余24只通过尾静脉注射羊抗大鼠Fx1A血清构建PHN大鼠模型。建模成功后，将PHN大鼠随机分为模型组、疏肝补肾方组和西药组，每组8只。分组后开始灌胃给药干预：疏肝补肾方组大鼠按11.7 g·kg-1·d-1剂量灌服疏肝补肾方药液；西药组大鼠灌服醋酸泼尼松（1 mg·kg-1·d-1）联合环磷酰胺（3 mg·kg-1·d-1）混悬液；空白对照组与模型组大鼠均灌服等量生理盐水。各组均每日灌胃1次，连续干预4周。于造模1周后（干预起始前）及干预1、2、4周时，收集大鼠24 h尿液，采用酶联免疫吸附测定法（enzyme-linked immunosorbent assay，ELISA）检测24 h尿蛋白定量（24 hour urinary protein quantification，24 h UP）。干预4周后，经下腔静脉采血，采用ELISA法检测血清白细胞介素6（interleukin-6，IL-6）水平；取大鼠肾组织，分别进行光镜与电镜病理形态学观察；采用实时荧光定量聚合酶链式反应（quantitative real-time polymerase chain reaction，qRT-PCR）法检测肾组织中转化生长因子β1（transforming growth factor-β1，TGF-β1）、Smad2、Smad7的mRNA表达水平。 结果干预4周后，疏肝补肾方组、西药组大鼠24 h UP水平均低于模型组，且西药组降低幅度更明显，差异具有统计学意义（P＜0.05）；疏肝补肾方组与西药组的24 h UP水平比较，差异无统计学显著性（P＞0.05）。各组大鼠血清IL-6水平比较，差异无统计学显著性（P＞0.05）。肾组织PAS染色光镜结果显示：与模型组相比，西药组和疏肝补肾方组大鼠肾小球增大、基底膜增厚、毛细血管襻僵硬等病理改变均有所减轻。电镜结果显示：西药组和疏肝补肾方组大鼠肾组织基底膜增厚程度缓解，足突融合范围缩小，仅见少量电子致密物稀疏沉积。分子生物学检测结果显示：与空白对照组比较，模型组大鼠肾组织中TGF-β1、Smad2的mRNA表达水平呈升高趋势，Smad7 mRNA表达水平呈降低趋势，但差异均无统计学显著性（P＞0.05）；西药组大鼠肾组织Smad7 mRNA表达水平显著降低（P＜0.05）；与模型组比较，疏肝补肾方组与西药组大鼠肾组织TGF-β1、Smad2的mRNA表达水平均降低，且Smad2 mRNA表达水平降低更为显著（P＜0.05）。 结论疏肝补肾方能够降低被动型Heymann肾炎大鼠的尿蛋白水平，减轻肾组织病理损伤，并可下调肾组织中TGF-β1与Smad2 mRNA的表达，影响TGF-β1/Smad信号通路，进而抑制肾组织纤维化进程，发挥延缓膜性肾病进展的作用。
Abstract:
ObjectiveTo observe therapeutic effects of liver-soothing kidney-invigorating prescription on rats suffering passive Heymann nephritis (PHN), and to discuss the mechanism of the prescription in preventing and treating MN. MethodsAll 32 male SD rats were randomly divided into a blank control group (n=8) and a model induction group (n=24). The model induction group received an injection of sheep anti-rat Fx1A serum via the tail vein to establish the PHN rat models. After successfully modeling, PHN rats were randomized into the model group, liver-soothing kidney-invigorating prescription group and Western medicine group with eight in each. The rats began to receive intragastric administration for intervention after grouping: liver-soothing kidney-invigorating prescription group was drenched with the solution of liver-soothing kidney-invigorating prescription at a dose of 11.7 g·kg-1·d-1; Western medicine group accepted the gavage of the suspension of prednisone acetate (1 mg·kg??·d??) combined with cyclophosphamide (3 mg·kg??·d??); the blank control group and the model group were administered with the same amount of physiological saline. All the groups accepted intragastric administration once each day, for four weeks in a row. Urine samples of the rats were collected for 24 hours at one week after modeling (before the start of intervention) and at 1, 2, and 4 weeks of intervention, ELISA was adopted to detect the levels of 24 h UP. After four weeks of the intervention, blood was collected from the inferior vena cava, ELISA was applied to measure the levels of IL-6 in the serum; renal tissue samples were collected from the rats for pathological morphological observation under light microscope and electron microscope, respectively. The expressions of TGF-β1, Smad2 and Smad7 mRNA in renal tissues were tested using qRT-PCR method. ConclusionLiver-soothing kidney-invigorating prescription could lower the levels of urinary protein and relieve renal histopatho-logical lesions in rats with PHN, and downregulate the expressions of TGF-β1 and Smad2 mRNA in the renal tissue, and affecting TGF-β1/Smad signaling pathway, thereby inhibiting renal tissue fibrosis and delaying the progression of MN.

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备注/Memo

备注/Memo:
山东省中医药科技发展计划（2013ZDZK-034）。李文圃（1995—），男，硕士学位，医师。研究方向：中医药治疗肾系疾病。
更新日期/Last Update: 2026-03-15