[1]赵丽华,康晓娜,韩小江.罗汉果玉竹颗粒对脂多糖诱导大鼠急性肺损伤中Fibronectin、Collagen I及α-SMA表达的影响[J].西部中医药,2026,39(03):28-32.[doi:10.12174/j.issn.2096-9600.2026.03.06]
　ZHAO Lihua,KANG Xiaona,HAN Xiaojiang.Effects of Luohanguo Yuzhu Granules on the Expressions of Fibronectin, Collagen I and α-SMA in Rats with LPS-induced ALI[J].Western Journal of Traditional Chinese Medicine,2026,39(03):28-32.[doi:10.12174/j.issn.2096-9600.2026.03.06]
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罗汉果玉竹颗粒对脂多糖诱导大鼠急性肺损伤中Fibronectin、Collagen I及α-SMA表达的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
28-32
栏目:
基础研究
出版日期:
2026-03-15

文章信息/Info

Title:
Effects of Luohanguo Yuzhu Granules on the Expressions of Fibronectin, Collagen I and α-SMA in Rats with LPS-induced ALI
作者:
赵丽华1, 康晓娜1, 韩小江2
1.内蒙古自治区国际蒙医医院药学部，内蒙古 呼和浩特 010065
2.内蒙古医科大学附属医院，内蒙古 呼和浩特 010059
Author(s):
ZHAO Lihua1, KANG Xiaona1, HAN Xiaojiang2
1.Department of Pharmacy, International Mongolian Hospital of Inner Mongolia, Hohehot 010065, China
2.Affiliated Hospital of Inner Mongolia Medical University, Hohehot 010059, China
关键词:
急性肺损伤; 罗汉果玉竹颗粒; 脂多糖; 纤连蛋白; I型胶原; 平滑肌肌动蛋白
Keywords:
acute lung injury; granules; lipopolysaccharide; fibronectin; collagen I; -smooth muscle actin
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2026.03.06
文献标志码:
A
摘要:
目的研究罗汉果玉竹颗粒对脂多糖（lipopolysaccharide，LPS）诱导的大鼠急性肺损伤（acute lung injury，ALI）模型的保护作用及其可能机制。 方法将58只SPF（specific pathogen free，SPF）级Wistar大鼠按体质量随机分为空白对照组（K组，10只）、模型对照组（M组，12只）、罗汉果玉竹颗粒低剂量组（LY-L组，3.24 g·kg-1，12只）、罗汉果玉竹颗粒高剂量组（LY-H组，6.48 g·kg-1，12只）和泼尼松阳性药物对照组（PN组，5.4 mg·kg-1，12只）。除K组外，其余各组大鼠均经尾静脉注射LPS溶液（6 mg·kg-1）。造模后，各给药组每日灌胃相应药物1次，给药体积为10 mL·kg-1；K组和M组给予等体积蒸馏水。连续给药7天后，腹腔注射10%水合氯醛（3.5 mL·kg-1）麻醉，腹主动脉取血，采用酶联免疫吸附测定（enzyme-linked immunosorbent assay，ELISA）检测血清白细胞介素6（interleukin-6，IL-6）含量；取肺组织，苏木精-伊红（hematoxylin-eosin，HE）染色观察病理变化，免疫组织化学法检测纤连蛋白（fibronectin，FN）、I型胶原（collagen I，COL1A1）及α平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）的表达水平。 结果与K组比较，M组大鼠肺泡结构破坏，肺泡间隔明显增宽，肺泡壁大面积充血、水肿及炎性细胞浸润；血清IL-6含量升高（P＜0.01），肺组织FN、COL1A1及α-SMA表达均增加（P＜0.01）。与M组比较，LY-L组和LY-H组大鼠血清IL-6含量降低（P＜0.01），肺组织病理损伤明显改善，FN、COL1A1及α-SMA表达均降低（P＜0.01）。Pearson相关分析显示，血清IL-6含量与肺组织FN、COL1A1及α-SMA表达均呈正相关（P＜0.01）。 结论罗汉果玉竹颗粒对LPS诱导的大鼠ALI具有保护作用，其机制可能与减轻肺组织炎症反应、抑制IL-6释放，进而下调肺组织FN、COL1A1及α-SMA的表达有关。
Abstract:
ObjectiveTo study the protective effects of Luohanguo (Siraitiae fructus) Yuzhu (Polygonati odorati rhizoma) granules on LPS-induced ALI in rats and its potential mechanism. MethodsAll 58 SPF-grade Wistar rats were randomized into the blank control group (K group, 10 rats), model control group (M group, 12 rats), low-dose Luohanguo Yuzhu granules group (LY-L group, 3.24 g·kg-1, 12 rats), high-dose Luohanguo Yuzhu granules group (LY-H group, 6.48 g·kg-1, 12 rats) and prednisone positive control group (PN group, 5.4 mg·kg-1, 12 rats) according to body weight. Except for K group, the rats in the remaining groups received LPS solution (6 mg·kg-1) via tail vein injection. After successfully modeling, the treatment groups received the corresponding drugs by gavage once daily, administration volume was 10mL·kg-1; K group and M group were given the same volume of distilled water. After seven consecutive days of administration, the rats were anesthetized with an intraperitoneal injection of 10% chloral hydrate (3.5 mL·kg-1), and blood samples were collected from the abdominal aorta, ELISA was adopted to detect the contents of IL-6; lung tissues were collected for hematoxylin-eosin (HE) staining to observe pathological changes. Immunohistochemistry (IHC) was adopted to measure the expressions of FN, COL1A1 and α-SMA. ResultsCompared with K group, M group observed disrupted alveolar structure, markedly thickened alveolar septa, and extensive congestion, edema, and inflammatory cell infiltration in the alveolar walls; an increase in the contents of IL-6 (P<0.01), the elevated expressions of FN, COL1A1 and α-SMA in lung tissue (P<0.01). Compared with M group, LY-L group and LY-H group demonstrated a decrease in the contents of IL-6 (P<0.01), a significant improvement in lung histopathological injury, the reduction in the expressions of FN, COL1A1 and α-SMA (P<0.01). Pearson correlation analysis showed that the contents of IL-6 were positively related to the expressions of FN, COL1A1 and α-SMA in lung tissue (P<0.01). Conclusion Luohanguo Yuzhu granules have a protective effect against LPS-induced ALI in rats, and its mechanism might be attributed to the attenuation of the inflammatory response in lung tissue, the inhibition of IL-6 release, and the subsequent downregulation of FN, COL1A1, and α-SMA expression in the lungs.

相似文献/References:

[1]脱承德,袁冰华,刘野,等.宣白承气汤灌肠对急性肺损伤患者ET和I-FABP的影响[J].西部中医药,2017,30(11):110.
　TUO Chengde,YUAN Binghua,LIU Ye,et al.Effects of XuanBai ChengQi Decoction Enema on ET and I-FABP of the Patients with Acute Lung Injury[J].Western Journal of Traditional Chinese Medicine,2017,30(03):110.

备注/Memo

备注/Memo:
内蒙古自治区自然科学基金项目［2020MS（LH）0812］。赵丽华（1982—），女，硕士学位，副主任药师。研究方向：药物分析与临床药学。
更新日期/Last Update: 2026-03-15