[1]黄小芮,刘建华,闫占峰,等.基于网络药理学与分子对接探讨苓桂术甘汤治疗良性阵发性位置性眩晕的机制[J].西部中医药,2026,39(03):108-114.[doi:10.12174/j.issn.2096-9600.2026.03.21]
　HUANG Xiaorui,LIU Jianhua,YAN Zhanfeng,et al.Network Pharmacology and Molecular Docking Based Exploration into the Mechanism of Lingguizhugan Decoction in the Treatment of Benign Paroxysmal Positional Vertigo[J].Western Journal of Traditional Chinese Medicine,2026,39(03):108-114.[doi:10.12174/j.issn.2096-9600.2026.03.21]
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基于网络药理学与分子对接探讨苓桂术甘汤治疗良性阵发性位置性眩晕的机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年03期
页码:
108-114
栏目:
二次研究
出版日期:
2026-03-15

文章信息/Info

Title:
Network Pharmacology and Molecular Docking Based Exploration into the Mechanism of Lingguizhugan Decoction in the Treatment of Benign Paroxysmal Positional Vertigo
作者:
黄小芮, 刘建华, 闫占峰, 王鑫, 刘思溟, 张琳婧, 朱雅静, 孙红, 丁倩
北京中医药大学东直门医院，北京 100007
Author(s):
HUANG Xiaorui, LIU Jianhua, YAN Zhanfeng, WANG Xin, LIU Siming, ZHANG Linjing, ZHU Yajing, SUN Hong, DING Qian
Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100007, China
关键词:
良性阵发性位置性眩晕; 苓桂术甘汤; 网络药理学; 分子对接
Keywords:
benign paroxysmal positional vertigo; decoction; network pharmacology; molecular docking
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2026.03.21
文献标志码:
A
摘要:
目的运用网络药理学和分子对接技术，探讨苓桂术甘汤治疗良性阵发性位置性眩晕（benign paroxysmal positional vertigo，BPPV）的作用机制。 方法分别在中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）、BATMAN-TCM数据库及TCMID数据库中检索，筛选苓桂术甘汤的活性成分及作用靶点；以“benign paroxysmal positional vertigo”为关键词，在人类基因数据库（the Human gene database，GeneCards）与在线人类孟德尔遗传数据库（online Mendelian inheritance in man，OMIM）中筛选BPPV疾病潜在靶点。运用Cytoscape 3.7.1软件构建药物-活性成分-作用靶点网络图，基于度值（degree）、中介中心性（betweenness centrality，BC）、接近中心性（closeness centrality，CC）筛选关键成分和核心靶点。利用Venny 2.1.0平台获取药物与疾病的交集靶点后，将其导入STRING在线数据库构建蛋白-蛋白互作（protein-protein interactions，PPI）网络；在Metascape数据库中进行基因本体论（gene ontology，GO）和京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）富集分析。采用GraphPad Prism 8软件作图，并运用Cytoscape软件对前20条KEGG通路进行网络可视化分析；对关键活性成分和核心靶点进行分子对接验证。 结果共筛选出苓桂术甘汤活性成分114个，药物与疾病交集靶点128个。关键活性成分包括槲皮素、山柰酚、左旋延胡索乙素等，核心靶点包括PTGS2、AR等。涉及的通路包括癌症通路、神经活性配体-受体相互作用通路、环磷酸腺苷信号通路等。分子对接结果显示，各复合物结合能均为负数，表明关键活性成分与核心靶点具有较强的结合活性。 结论苓桂术甘汤可能通过多靶点、多通路协同发挥治疗 BPPV的作用，为苓桂术甘汤的临床应用及后期深入研究提供方向。
Abstract:
ObjectiveTo investigate the mechanism of action of Lingguizhugan decoction in the treatment of benign paroxysmal positional vertigo (BPPV) using network pharmacology and molecular docking technology. MethodsThe active components and corresponding targets of Lingguizhugan decoction were retrieved and screened from TCMSP, BATMAN-TCM database, and the TCMID database respectively. Using "benign paroxysmal positional vertigo" as a keyword, potential targets for BPPV were screened in GeneCards and OMIM database. The drug-active component-target network was constructed using Cytoscape 3.7.1 software. Key components and core targets were screened based on degree, BC, and CC. After obtaining the intersecting targets between the drug and the disease using the Venny 2.1.0 platform, these targets were imported into the STRING online database to construct a PPI network. GO and KEGG enrichment analyses were performed using the Metascape database. Graphical representations were generated using GraphPad Prism 8 software, and network visualization analysis of the top 20 KEGG pathways was conducted using Cytoscape software. Molecular docking validation was performed between the key active components and core targets. ResultsA total of 114 active components of Lingguizhugan decoction and 128 intersecting targets between the drug and the disease were identified. Key active components included quercetin, kaempferol, and levo-tetrahydropalmatine, while core targets included PTGS2 and AR. The involved pathways included the cancer pathway, the neuroactive ligand-receptor interaction pathway, and the cyclic adenosine monophosphate (cAMP) signaling pathway. Molecular docking results showed that the binding energies of all complexes were negative, indicating strong binding activity between the key active components and the core targets. Conclusion Lingguizhugan decoction may exert a synergistic therapeutic effect on BPPV through multiple targets and pathways, which could provide the direction for its clinical application and future in-depth research of the decoction.

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备注/Memo

备注/Memo:
国家自然科学基金（81904261）。黄小芮（1996—），女，硕士学位，医师。研究方向：中西医治疗耳鼻咽喉疾病。
更新日期/Last Update: 2026-03-15