茯苓酸对小鼠黑色素瘤细胞增殖、迁移和侵袭的影响及机制研究-《西部中医药》

文章信息/Info

Title:: The Effect of Pachymic Acid on the Proliferation, Migration and Invasion of Mouse Melanoma Cells and Study on the Mechanism

Author(s):: ZHAO Tingxiu, LAN Yuqi, JIN He, HE Yanli, DU Biaoyan; School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

Keywords:: pachymic acid; mouse melanoma; proliferation; migration; invasion; epithelial-mesenchymal transition; mice; animal experiments

摘要:: 目的研究茯苓酸（pachymic acid，PA）对小鼠黑色素瘤B16细胞增殖、迁移和侵袭的影响及其可能机制。方法采用CCK8法和细胞克隆形成实验检测不同浓度（20、40、80 μmol/L）PA对B16细胞增殖的影响；细胞划痕和Transwell小室实验观察PA对B16细胞迁移和侵袭能力的影响；Western Blot法和免疫荧光法检测PA对B16细胞的上皮-间质转化相关蛋白表达的变化。结果PA可以明显抑制B16细胞增殖，并呈一定的浓度依赖性关系（P＜0.01）；PA干预24 h后可以显著抑制B16细胞的迁移和侵袭能力（P＜0.01）；40 μmol/L的PA能上调B16细胞的E-cadherin蛋白表达（P＜0.05）并下调Vimentin蛋白的表达（P＜0.05）；免疫荧光法检测显示40 μmol/L的PA可明显降低B16细胞Vimentin蛋白的表达。结论PA可以抑制B16细胞的增殖、迁移和侵袭，其机制可能与上调B16细胞的E-cadherin蛋白表达，降低Vimentin蛋白表达，从而抑制B16细胞的上皮-间质转化有关。

Abstract:: ObjectiveTo study the effects of pachymic acid (PA) on the proliferation, migration and invasion of mouse melanoma B16 cells and its possible mechanisms. MethodsCCK8 assay and cell clone formation assay were used to detect the effects of different concentrations (20, 40, 80 μmol/L) of PA on the proliferation of B16 cells; cell scratch and Transwell assays were used to observe the effects of PA on the migra-tion and invasive ability of B16 cells; and Western Blot and immunofluorescence assays to detect the effects of PA on the expression of epithelial-mesenchymal transition-related protein of B16 cells. ResultsPA could obviously inhibit the proliferation of B16 cells in a concentration-dependent manner (P<0.01); PA could remarkably inhibit the migration and invasion ability of B16 cells after 24 h of intervention (P<0.01); 40 μmol/L PA up-regulated the expressions of E-cadherin protein (P<0.05) and down-regulated the expression of Vimentin protein of B16 cells (P<0.05); immunofluorescence assay showed that 40 μmol/L PA apparently decreased the expression of Vimentin protein in B16 cells. ConclusionPA can inhibit the proliferation, migration and invasion of B16 cells, and its mechanism may be related to the up-regulation of E-cadherin protein expression and the reduction of Vimentin protein expression in B16 cells, thus inhibiting the epithelial-mesenchymal transition of B16 cells.