[1]王翠兰.乳奥康对乳腺癌细胞株MCF-7 增殖及BCL-2 表达的影响[J].西部中医药,2013,26(01):4-6.
　WANG Cuilan.Effects of RuA oKang on Expression of BCL-2 and Proliferation of Mammary Cancer Cell Line MCF-7[J].Western Journal of Traditional Chinese Medicine,2013,26(01):4-6.
点击复制

乳奥康对乳腺癌细胞株MCF-7 增殖及BCL-2 表达的影响()
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年01期
页码:
4-6
栏目:
出版日期:
2013-01-15

文章信息/Info

Title:
Effects of RuA oKang on Expression of BCL-2 and Proliferation of Mammary Cancer Cell Line MCF-7
文章编号:
1004-6852(2013)01-0004-03
作者:
王翠兰
大庆市龙南医院，黑龙江 大庆 163000
Author(s):
WANG Cuilan
Daqing General Hospital Group Longnan Hospital, Daqing 163000, China
关键词:
乳腺癌细胞 MCF-7; 乳奥康; BCL-2
Keywords:
mammary cancer cell MCF-7;  RuAoKang;  BCL-2
分类号:
R737.3
文献标志码:
A
摘要:
目的：探讨乳奥康对乳腺癌细胞 MCF-7 增殖的影响及其对 BCL-2 表达的影响。方法：培养乳腺癌细胞株 MCF-7，采用 MTT 法检测乳奥康在不同浓度和不同作用时间下对乳腺癌细胞 MCF-7 增殖的影响；Western blotting 法检测乳奥康对乳腺癌细胞 BCL-2 表达的影响。结果：MTT 结果显示，乳奥康具有对 MCF-7细胞抑制的作用，其抑制作用和浓度、作用时间呈正相关。MCF-7 细胞经乳奥康作用，BCL-2 的表达逐渐降低。结论：乳奥康对乳腺癌细胞 MCF-7 的增殖具有抑制作用，其作用机制可能是通过抑制乳腺癌细胞 BCL-2 的表达，从而启动炎症抑制相关机制，使肿瘤细胞发生凋亡。
Abstract:
Objective: To investigate the effects of RuAoKang on proliferation of mammary cancer cell line MCF-7 and expression of BCL-2. Method: Mammary cancer cell line MCF-7 was cultivated and the effects of RuA oKang on proliferation of MCF-7 were detected under different concentrations and in different times with MTT assay; the effects of RuAoKang on expression of BCL-2 were surveyed with Western blotting method. Result: The results of MTT demonstrated that RuAoKang could inhibit the proliferation of MCF-7 and the inhibition of RuA oKang was positively associated to the concentration and time. Expression of BCL-2 was decreased gradually after RuAoKang acted on MCF-7. Conclusion: RuAoKang can inhibit the proliferation of mammary cancer cell MCF-7, which might stimulate the anti-inflammation mechanism and induce the apoptosis of tumor cell though inhibiting the expression of mammary cancer cell BCL-2.

参考文献/References:

［1］ Haile RW，Yochim JM，Cortessis VK，et al. A molecular/epidemiologic analysis of expression of cyclooxygenases 1 and 2，use of nonsteroidal antiinflammatory drugs，and risk of colorectal adenoma ［J］.Clin Colorectal Cancer，2005，4(6)：390-395.
［2］ Chan AT，Giovannucci EL，Meyerhardt JA，et al.Long-term use of aspirin and nonsteroidal anti-inflammatory drugs and risk of colorectal cancer［J］.JA MA，2005，294(8)：914-923.
［3］ Kim KY，Seol JY，Jeon GA，et al.The combined treatment of aspirin and radiation induces apoptosis by the regulation of bcl-2 and caspase-3 in human cervical cancer cell［J］.Cancer Lett，2003，189(2)：157-166.
［4］ Hayden MS，Ghosh S.Shared principles in NF-KappaB signaling ［J］.Cell，2008，132(3)：344-362.
［5］ Schoemaker MH，Ros JE，Homan M，et al. Cytokine regulation of anti apoptotic genes in rat hepatocytes：NF-kappa B-regulated inhibitor of apoptosis protein 2(cIAP2) prevents apop tosis［J］.J Hepatol，2002，36(6)：742-750.
［6］ Husain SS，Szabo IL，Tamawski AS.NSAID inhibition of GI cancer growth：clinical implications and molecular mechanisms of action［J］.Am J Gastroenterol，2009，97(3)：542-553.
［7］ Escárcega RO，Fuentes-Alexandro S，Garcia-Carrasco M，et al.The transcription factor nuclear factor-kappa B and cancer ［J］.Clinical Oncology，2007，19(2)：154-161.

备注/Memo

备注/Memo:
收稿日期：2012-08-22
作者简介：王翠兰(1962—)，女，副高级检验师。研究方向：临床检验学。
更新日期/Last Update: 2013-01-15