[1]孟陆亮,毋荣荣,史正刚,等.平痫冲剂对幼年海人酸颞叶癫痫模型大鼠学习记忆行为的影响*[J].西部中医药,2013,26(04):19-22.
　MENG Luliang,WU Rongrong,et al.Effects of PingXian Granules on the Learning and Memory Behavior of Young Rats with Temporal Lobe Epilepsy[J].Western Journal of Traditional Chinese Medicine,2013,26(04):19-22.
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平痫冲剂对幼年海人酸颞叶癫痫模型大鼠学习记忆行为的影响*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年04期
页码:
19-22
栏目:
出版日期:
2013-04-15

文章信息/Info

Title:
Effects of PingXian Granules on the Learning and Memory Behavior of Young Rats with Temporal Lobe Epilepsy
文章编号:
1004-6852(2013)04-0019-04
作者:
孟陆亮1，2，毋荣荣1，史正刚1，张加权1，陈开兵3，李永新4
1 甘肃中医学院，甘肃 兰州 730000；2 渭南职业技术学院；3 甘肃中医学院附属医院；4 甘肃省中医院
Author(s):
MENG Luliang1, 2, WU Rongrong1, SHI Zhenggang1, ZHANG Jiaquan1, CHEN Kaibing3, LI Yongxin4
1 Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China; 2 W einan V ocational & Technical College; 3 University Hospital of Gansu Traditional Chinese Medicine; 4 Gansu Province Hospital of Traditional Chinese Medicine
关键词:
平痫冲剂; 海人酸; 颞叶癫痫; 幼年; 学习记忆行为; 脑电图
Keywords:
PingXian granules; kainic acid; temporal lobe epilepsy;  young;  learning and memorybehavior; EEG
分类号:
R742.1
文献标志码:
A
摘要:
目的：建立幼年海人酸颞叶癫痫模型并给予平痫冲剂治疗，探讨对其学习记忆行为的影响。方法：将 60 只 40 日龄 Wistar 大鼠，随机分为正常对照组（A 组）、模型组（B 组）、阳性对照组（C 组）及平痫冲剂低（D 组）、中（E 组）、高剂量组（F 组）。除 A 组外，其余各组脑立体定位注射海人酸诱发颞叶癫痫。A 组正常饲养，B 组灌服等量生理盐水，D、E、F 组灌服平痫冲剂，低、中、高给药剂量按人(70 kg)用药剂量的 3 倍、6 倍、12 倍给药，分别为 0.7、1.4、2.8 g/(kg·d)，C 组灌服等量卡马西平，均每日灌胃 1 次,连续灌胃 1 月。实验过程中监测 大鼠症状的改变，深部脑电图观察颞叶癫痫的病理过程及治疗前后的脑电变化，利用 Morris 水迷宫和八臂迷宫实验评价各组大鼠的学习记忆行为。结果：①大鼠症状的改变：海人酸注射后半小时到 6 小时大鼠出现急性发作，进而逐渐缓减，2 个月后出现自发发作。平痫冲剂治疗可抑制癫痫发作及其发展；②电生理学改变：脑电图于 24 小时和 2 个月后均表现为典型的癫痫发作电活动及发作间期表现。治疗后癫痫波持续时间缩短；③学习记忆行为的变化：海人酸颞叶癫痫大鼠学习记忆能力明显降低。平痫冲剂可缩短其逃避潜伏期，提高穿越原平台次数，减少参考记忆和工作记忆错误。结论：海人酸模型是模拟人类颞叶癫痫较为理想的动物模型；平痫冲剂对癫痫发作及脑电活动具有抑制作用，并且可以改善其学习记忆行为能力。
Abstract:
Objective: To establish the temporal lobe epilepsy models with kainic acid and explore the effects of PingXian granules on the learning and memory behavior of rats. Method: All 60 rats were randomized into normal control group (A), model group (B), positive control group (C), low, middle and high doses groups of PingXian granules (D, E and F). Except A, others groups were injected with kainic acid to build models with temporal lobe epilepsy. Rats in A group were fed normally, B group were given with the same amount of physiological saline, D, E and F were administered with PingXian granules in the doses of 0.7, 1.4 and 2.8 g/(kg·d), C received the same amounts of carbamazepine through intragastric administration, each day one time, for one consecutive month. The changes of the symptoms in rats were monitored, pathological courses of temporal lobe epilepsy and changes of EEG were observed before and after treating with depth EEG, learning and memory behavior of rats in groups were assessed with Morris water maze and eight-arm radial maze. Result: ① Changes of the symptoms in rats: The rats were triggered acute attack in half an hour to six hours after injecting kainic acid, relieved gradually and attacked spontaneously in two months. PingXian granules could inhibit the onset and development of epilepsy; ② Electrophysiological changes:EEG in 24 hours and two months showed typical electrical activity of seizure and attack performance. After treating the duration of epilepsy wave was shortened; ③ Changes of learning and memory behavior: Learning and memory behavior of temporal lobe epilepsy rats induced by kainic acid were decreased obviously. PingXian granules could shorten escape latency, raise the times of platform crossing and reduce the mistakes of reference memory and working memory. Conclusion: Models established with kainic acid are ideal animals imitating human suffering from temporal lobe epilepsy; PingXian granules could inhibit the attack of epilepsy and electrical activity of brain, which could improve learning and memory ability.

备注/Memo

备注/Memo:
收稿日期：2013-01-29 * 基金项目 ：2010年甘肃省中医药英才基金项目 (编号GZK-2010-61) 作者简介：孟陆亮(1968—)，男，教授，主任医师。研究方向：儿童神经精神及呼吸消化疾病的中西医结合诊治。
更新日期/Last Update: 2013-04-15