[1]杨频,杨丽霞,刘铜华△,等.止消通脉宁对TGF-β1诱导的HK-2细胞Smad 2、3、7 mRNA表达的影响[J].西部中医药,2013,26(09):16-19.
　YANG Pin,YANG Lixia,LIU Tonghua,et al.The Effects of ZhiXiao TongMaiNing on Smad 2, Smad 3 and Smad 7 mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β1[J].Western Journal of Traditional Chinese Medicine,2013,26(09):16-19.
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止消通脉宁对TGF-β1诱导的HK-2细胞Smad 2、3、7 mRNA表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年09期
页码:
16-19
栏目:
出版日期:
2013-09-15

文章信息/Info

Title:
The Effects of ZhiXiao TongMaiNing on Smad 2, Smad 3 and Smad 7 mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β1
文章编号:
1004-6852(2013)09-0016-04
作者:
杨频1，杨丽霞2，刘铜华3△，吴丽丽3，Margetts Peter Joseph4
1 甘肃省中医学校，甘肃 兰州 730050； 2 甘肃省中医药研究院； 3 北京中医药大学； 4 McMaster University， Hamilton Ontario， Canada T3309
Author(s):
YANG Pin1, YANG Lixia2, LIU Tonghua 3△, WU Lili3, Margetts Peter Joseph4
1 GanSu Provincial TCM School, Lanzhou 730050, China; 2 GanSu Provincial Academy of Chinese Medicine; 3 Beijing University of Chinese Medicine; 4 McMaster University
关键词:
止消通脉宁; 转化生长因子-β1; HK-2细胞; Smad 2 mRNA; Smad 3 mRNA; Smad 7 mRNA
Keywords:
ZhiXiao TongMaiNing;  TGF-β1;  HK-2 cells;  Smad 2 mRNA;  Smad 3 mRNA;  Smad 7 mRNA
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨止消通脉宁对转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)Smad 2、Smad 3、Smad 7的影响。方法：将HK-2细胞用含10%胎牛血清的DMEM/F12(1：1)培养基培养；实验分为6组：空白对照组、单纯TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL +10%空白血清)、中药含药血清低剂量组(TGF-β110 ng/mL+10%低剂量止消通脉宁含药血清)、中药含药血清中剂量组(TGF-β110 ng/mL+10%中剂量止消通脉宁含药血清)、中药含药血清高剂量组(TGF-β110 ng/mL+10%高剂量止消通脉宁含药血清)。药物干预24小时后，荧光定量PCR检测Smad 2、smad 3、Smad 7的mRNA表达。结果：HK-2细胞经TGF-β1诱导后，Smad 2、smad 3的mRNA表达显著上升，Smad 7的mRNA表达下降，与空白对照组相比有显著性差异(P＜0.05)，但经止消通脉宁含药血清干预后， Smad 2、smad 3的mRNA表达逐步下降，Smad 7的mRNA表达上调，与单纯TGF-β1诱导组相比有显著性差异(P＜0.05)。而空白血清无此作用。结论：止消通脉宁能够调控TGF-β1诱导的人肾小管上皮细胞转分化Smad信号通路mRNA表达的作用，在一定程度上具有抑制肾间质纤维化的作用。
Abstract:
Objective: To explore the effects of ZhiXiao TongMaiNing on Smad 2, Smad 3 and Smad 7 of human renal tubular epithelial cells (HK-2) which were induced by transforming growth factor (TGF-β1). Method: HK-2 cells were cultured in the media of DMEM/F12 (1:1) containing 10% fetal bovine serum; the cells were divided into six groups: blank control group, TGF-β1 group(TGF-β110 ng/mL), blank serum control group(TGF-β1 10 ng/mL+10% blank serum), low dose group of TCM medicated serum(TGF-β110 ng/mL+10% low dose medicated serum of ZhiXiao TongMaiNing), moderate dose group of TCM medicated serum (TGF-β1 10 ng/mL+10% moderate dose medicated serum of ZhiXiao TongMaiNing) and high dose group of TCM medicated serum(TGF-β1 10 ng/mL+10% high dose medicated serum of ZhiXiao TongMaiNing). After intervened for 24 hours, Smad 2, Smad 3 and Smad 7 mRNA expressions were detected with fluorescence quantitative PCR. Result: Smad2 and Smad3 mRNA expressions were raised significantly, Smad 7 mRNA expressions were decreased when HK-2 cells were induced with TGF-β1, it had significant difference compared with blank control group(P＜0.05), but after intervened with medicated serum of ZhiXiao TongMaiNing, Smad 2 and Smad 3 mRNA expressions were lowered gradually, Smad 7 mRNA expressions were improved, it had statistical meaning compared with TGF-β1 group (P＜0.05). Blank serum presented no effects. Conclusion: ZhiXiao TongMaiNing could regulate mRNA expressions of trans-differentiated Smad signal channel of HK-2 cells induced with TGF-β1 and inhibit the fibrosis of renal tubular epithelial cells to a certain extent.

相似文献/References:

[1]朱春晖,康开彪,杨丽霞,等.糖耐康对TGF-β1诱导的HK-2细胞VEGF mRNA表达的影响*[J].西部中医药,2013,26(09):13.
　ZHU Chunhui,KANG Kaibiao,YANG Lixia,et al.Effects of TangNaiKang on VEGF mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β1[J].Western Journal of Traditional Chinese Medicine,2013,26(09):13.
[2]舒畅,杨丽霞,程涛,等.基于 Smads 信号通路探讨止消通脉宁干预 TGF-β1诱导 HK-2 细胞转分化的研究*[J].西部中医药,2013,26(06):10.
　SHU Chang,YANG Lixia,CHENG Tao,et al.Research on Intervention of ZhiXiao TongMaiNing to Trans-differentiation of Human Renal Tubular Epithelial HK-2 Cell Induced by TGF-β1 Based on Smads Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2013,26(09):10.
[3]任虹,杨丽霞,吴丽丽,等.止消通脉宁干预TGF—β1诱导HK—2细胞增殖的研究[J].西部中医药,2012,25(08):10.
　REN Hong,YANG Li-xia,WU Li-li,et al.Intervention of ZhiXiao TongMaiNing on Proliferation of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β1[J].Western Journal of Traditional Chinese Medicine,2012,25(09):10.

备注/Memo

备注/Memo:
收稿日期：2013-03-13 *基金项目：科技部国际科技合作项目(编号2009DFA31520)，北京中医药大学创新团队项目(编号2011-CXTD-19) 作者简介：杨频(1977—)，女，讲师。研究方向：中医药基础研究。 △通讯作者：刘铜华(1963—)，男，博士研究生导师，博士学位，教授。研究方向：糖尿病及其并发症的中医药防治。
更新日期/Last Update: 2013-09-15