[1]马琴国,安培坤,王引权△.不同采样期党参炔苷的含量动态变化分析*[J].西部中医药,2014,27(02):23-25.
　MA Qinguo,AN Peikun,WANG Yinquan.Dynamic Changes of the Contents of Lobetyolin Contained in DangShen at Different Sampling Periods[J].Western Journal of Traditional Chinese Medicine,2014,27(02):23-25.
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不同采样期党参炔苷的含量动态变化分析*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年02期
页码:
23-25
栏目:
出版日期:
2014-02-15

文章信息/Info

Title:
Dynamic Changes of the Contents of Lobetyolin Contained in DangShen at Different Sampling Periods
文章编号:
1004-6852(2014)02-0023-03
作者:
马琴国1，安培坤1，王引权2△
1 甘肃省中医院，甘肃 兰州 730050； 2 甘肃中医学院
Author(s):
MA Qinguo1, AN Peikun1, WANG Yinquan2△
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu University of Traditional Chinese Medicine
关键词:
不同采样期; 党参; 党参炔苷; 高效液相
Keywords:
different sampling periods;  DangShen;  lobetyolin;  HPLC
分类号:
R282.5
文献标志码:
A
摘要:
目的：分析不同采样期党参炔苷的含量。方法：炔苷含量采用高效液相色谱分析，TC-C18色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-水(22∶78)为流动相,流 速：1.0 mL/min。检测波长267 nm，柱温25℃。结果：党参炔苷在1.12～0.80 μg/mL范围内呈良好的线性关系，r=0.999 6，精密度RSD为1.28%(n=6)，重复性RSD为1.9%，平均回收率为99.50%，RSD为1.90%(n=6)，测定了不同采收期党参中党参炔苷的含量范围为0.87～3.00 mg/g。结论：分析了不同采样期党参炔苷的含量，为党参规范化栽培提供参考。
Abstract:
Objective: To analyze the contents of lobetyolin in DangShen［Codonopsis pilosula(Franch.)Nannf.］ at different sampling periods. Methods: The contents of lobetyolin were analyzed by HPLC, TC-C18 chromatographic column (4.6 mm×250 mm, 5 μm), acetonitrile-water (22:78) were as the mobile phase, flow rate: 1.0mL/min. Detection wavelength was 267 nm, the temperature: 25℃. Results: Lobetyolin showed better linear relationship in the range between 1.12 to 0.80 μg/mL, r=0.999 6, accuracy RSD was 1.28%(n=6), repetition RSD was 1.9%, average recovery rate was 99.50%, RSD was 1.90% (n=9), the contents of lobetyolin ranged from 0.87 to 3.00mg/g at different sampling periods.Conclusion:The contents of lobetyolin are analyzed in different sampling periods in order to provide reference for standardized cultivation of DangShen.

相似文献/References:

[1]王宇,张玉兰△.HPLC法测定党参中5-羟甲基糠醛的含量[J].西部中医药,2014,27(02):31.
　WANG Yu,ZHANG Yulan.Determination of the Contents of 5-hydroxymethylfurfural in DangShen by HPLC[J].Western Journal of Traditional Chinese Medicine,2014,27(02):31.

备注/Memo

备注/Memo:
收稿日期：2013-05-13*基金项目：甘肃省教育厅科研项目(编号KY07-20)作者简介：马琴国(1976—)，男，硕士学位，药师。研究方向：中药资源综合开发与质量研究。△通讯作者：王引权(1963—)，男，博士学位，教授。研究方向：药用植物栽培生理生态研究。
更新日期/Last Update: 2014-02-15