[1]刘晓青.市售蕲蛇样品的传统鉴定与分子生物学鉴定特征[J].西部中医药,2014,27(05):16-18.
　LIU Xiaoqing.Characteristics of Commercial Ancistrodon Acutus Identified by Traditional Method and Molecular Biology[J].Western Journal of Traditional Chinese Medicine,2014,27(05):16-18.
点击复制

市售蕲蛇样品的传统鉴定与分子生物学鉴定特征
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年05期
页码:
16-18
栏目:
出版日期:
2014-05-15

文章信息/Info

Title:
Characteristics of Commercial Ancistrodon Acutus Identified by Traditional Method and Molecular Biology
文章编号:
1004-6852(2014)05-0016-03
作者:
刘晓青
沙河市人民医院药剂科，河北 沙河 054100
Author(s):
LIU Xiaoqing
Department of Pharmacy of Shahe Municipal People′s Hospital, Shahe 054100, China
关键词:
蕲蛇; 鉴定; PCR技术
Keywords:
ancistrodon acutus;  identification;  PCR technique
分类号:
R282.5
文献标志码:
A
摘要:
目的：探讨不同方法对市售蕲蛇样品真伪特征鉴别结果的准确性。方法：对来自不同销售公司蕲蛇样品分别采用传统鉴别方法和分子生物学方法进行鉴定。结果：采集三个城市医药销售公司共30个蕲蛇样品，传统外观和显微镜方法鉴定30个样品中有25个正品，5个伪品，合格率为83.3%；用分子生物学方法从市售蕲蛇样品中抽提线粒体DNA，采用PCR方法进行特异性引物扩增。30个蕲蛇样品中合格品20个，不合格品10个，合格率为66.67%。结论：传统鉴别方法鉴别市售蕲蛇样品特异性较低，分子生物学方法检测简单、准确、快捷，对规范市售蕲蛇中药材市场具有广阔前景。
Abstract:
Objective: To investigate the accuracy of different methods in distinguishing commercial ancistrodon acutus. Methods: The samples from different companies were identified with traditional method and the method of molecular biology respectively. Results: Altogether 30 kinds of samples from three pharmaceutical companies were collected. the results showed that there were 25 kinds of authentic ones and five counterfeit ones, qualification rate was 83.3%; DNA of mitochondria were extracted from the samples by the method of molecular biology, specific primers were amplified by PCR method. There were 20 kinds of qualified goods and ten kinds of unqualified ones, qualification rate was 66.67%. Conclusion: Traditional identification method is of low specificity, the method of molecular biology is simple, accurate and rapid, it demonstrates broad prospect to regulate the marketing of ancistrodon acutus.

相似文献/References:

[1]陈有智,罗文蓉,侯 嘉,等.不同产地当归的鉴定学研究[J].西部中医药,2012,25(05):25.
　CHEN You-zhi,LUO Wen-rong,HOU Jia,et al.Study on Identification of DangGui from Different Habitats[J].Western Journal of Traditional Chinese Medicine,2012,25(05):25.
[2]刘秀菊,李 炀.兰州百合与药用百合生药鉴定比较[J].西部中医药,2016,29(07):34.
　LIU Xiuju,LI Yang.Comparison and Identification of Crude Drug Lanzhou Lily and Officinal Lily[J].Western Journal of Traditional Chinese Medicine,2016,29(05):34.
[3]刘青,钟迎春,顿珠,等.基于DNA条形码ITS对西藏不同地理居群红景天的鉴定研究[J].西部中医药,2024,37(09):42.[doi:10.12174/j.issn.2096-9600.2024.09.09]
　LIU Qing,ZHONG Yingchun,DUN Zhu,et al.Identification of Hongjingtian from Different Geographical Populations in Xizang Based on DNA Bar Code ITS[J].Western Journal of Traditional Chinese Medicine,2024,37(05):42.[doi:10.12174/j.issn.2096-9600.2024.09.09]

备注/Memo

备注/Memo:
收稿日期：2013-07-20作者简介：刘晓青(1971—)，女，副主任药师。研究方向：药品鉴定。
更新日期/Last Update: 2014-05-15