气相色谱法测定辛芷滴鼻液中龙脑和异龙脑的含量-《西部中医药》

文章信息/Info

Title:: Determination of Borneol and Isoborneol in XinZhi Nasal Drops by Gas Chromatography

Author(s):: WEI Li1, LIU Guangbin2△; 1 Suzhou District Center for Disease Control and Prevention, Jiuquan 735000, China;2 Jiugang Hospital

Keywords:: XinZhi nasal drops; gas chromatography; borneol; isoborneol; content determination

摘要:: 目的：建立辛芷滴鼻液中龙脑和异龙脑的含量测定方法。方法：采用气相色谱法测定，DB-WAX色谱柱（30 m×0.53 mm，1.0 μm），载气为氮气，分流比为10∶1，柱温为140℃，进样口温度为180℃，检测器温度为200℃。结果：龙脑和异龙脑分别在17.6～282.0、11.8～188.4 μg/mL范围内呈良好的线性关系（r＝0.999 9、r＝0.999 9，n＝6）；平均加样回收率分别为98.91%和99.45%（RSD为1.36%、1.53%，n＝9）。结论：本法简便、准确、灵敏度高、重复性好，可用于辛芷滴鼻液的质量控制。

Abstract:: Objective: To establish content determination method of borneol and isoborneol in XinZhi nasal drops. Methods: Gas chromatography was adopted to detect DB-WAX chromatographic column (30 m×0.53 mm, 1.0 μm), carrier gas was nitrogen, split ratio 10:1, column temperature 140℃, inlet temperature 180℃ and detector temperature 200℃. Results: Borneol and isoborneol showed better linear relationship in the ranges between 17.6 and 282.0, 11.8 and 188.4 μg/mL(r=0.999 9, r=0.999 9, n=6); average recovery rates were 98.91% and 99.45% respectively (RSD=1.36%, 1.53%, n=9). Conclusion: The method, simple, accurate, high sensitivity and good reproducible, could be used for quality control of XinZhi nasal drops.