输合配穴针刺法促进脂多糖LPS早产鼠脑损伤中MAG及NgR表达的研究-《西部中医药》

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Title:: Study on “Stream-sea Point Combination” Needling in Promoting the Expressions of MAG and NgR in Premature Rats with LPS-induced Cerebral Injury

文章编号:: 1004-6852(2018)06-0043-04

作者:: 李楠，胡晓丽，王雪峰△; 辽宁中医药大学附属医院，辽宁沈阳 110032

Author(s):: LI Nan, HU Xiaoli, WANG Xuefeng△; The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110033, China

关键词:: 脑损伤; 输合配穴; 髓鞘相关糖蛋白; 动物实验

Keywords:: cerebral injury; stream-sea point combination; MAG; animal experiment

分类号:: R245

文献标志码:: A

摘要:: 目的：探讨“输合配穴”针刺法对脂多糖(LPS)早产鼠脑损伤中髓鞘相关糖蛋白（MAG）及NgR表达的影响。方法：LPS组孕鼠(n=10)从孕17天开始到出生每12小时注射200 μg/kg，而生理盐水组孕鼠(n=3)腹腔注射同剂量的生理盐水。将LPS组新生仔鼠随机分为针刺组(n=30)和非干预组(n=20)，生理盐水组孕鼠新生仔鼠分为对照组。针刺组进行针刺治疗，所有新生乳鼠从生后第1天至第21天每天接受机能和认知发育测试。于出生后第7、14、21天对乳鼠脑组织MAG及NgR进行蛋白免疫印迹法检测。神经发育测试数据采用重复测量方差分析方法。结果：平面翻正、悬崖回避及惊愕反射针刺组、非干预组与对照组比较，差异有统计学意义(P＜0.05)。前肢放置测试针刺组与对照组比较，差异有统计学意义(P＜0.05)，非干预组与对照组比较，差异有统计学意义(P＜0.01)。悬崖回避测试针刺组与非干预组比较，差异有统计学意义(P＜0.05)。悬吊实验、旷野实验3组比较，差异无统计学意义(P＞0.05)。负趋地性测试和耳朵抽搐测试这2项未测出结果。在脂多糖新生乳鼠生后7天即可见MAG的表达，生后14天逐渐升高，生后21天逐渐下降但仍高于对照组。生后7天即可见NgR的表达，生后14天至生后21天NgR的表达逐渐增强。结论：通过对孕鼠腹腔注射脂多糖后可引起乳鼠脑白质损伤，但是并不能出现与中度至重度脑瘫患儿一致的脑性瘫痪表型，通过神经行为学鉴定证明“输合配穴”针刺法治疗有效。

Abstract:: Objective: To discuss the effects of “stream-sea point combination” needling on the expressions of myelin associated glucoprotein(MAG) and NgR of premature rats with lipopolysaccharide (LPS)-induced cerebral injury. Methods: Pregnant rats(n=10) of LPS group were injected with LPS, 200 μg/kg, every 12 hours from the 17th day after the gestation to the birth day, while pregnant rats(n=3) of physiological saline group accepted peritoneal injection of physiological saline in the equivalent dose. Neonatal rats in LPS group were randomized into the needling group (n=30) and non intervention group (n=20), newborn rats of the pregnant rats in physiological saline group were chosen as the control group. The needling group accepted the needling, all neonatal rats received the test of function and cognitive development from the first day to 21st day after the birth. The expressions of MAG and NgR in cerebral tissue of neonatal rats were measured at the seventh, 14th and 21st day after the birth by western blot. The data of neural development test were dealt with repetitive measure analysis of variance (ANOVA). Results: The difference had statistical meaning when the needling group and non intervention group were compared with control group in righting reflex, cliff avoidance test and startle response(P＜0.05). The difference had statistical meaning when the needling group was compared with the control group in forelimb placement test(P＜0.05), when non intervention group was compared with the control group, there was a significant difference(P＜0.05). When the needling group was compared with non intervention group in cliff avoidance test, the difference had statistical meaning (P＜0.05). The difference had no statistical meaning when three groups were compared with each other in suspension experiment and field experiment(P＞0.05). No results were detected in negative geotaxis reflex and ear twitching test. The expressions of MAG could be found when the neonatal rats in LPS group were born in seven days, the expressions of MAG raised gradually in 14 days after the birth, while decreased in 21 days after the birth, still higher than these inthe control group. The expressions of NgR could be seen when the rats were born in seven days, and increased gradually from the 14 day to 21 days after the birth. Conclusion: Peritoneal injection of LPS to the pregnant rats could induce white matter damage(WMD) of neonatal rats, while they couldn′t appear cerebral palsy phenotype which was identical to children with moderate to severe cerebral palsy, and stream-sea point combination needling is effective to treat the disease which is found by neurobehavioral identification.

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

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