[1]韦孝晨,严彪,徐乃玉,等.臭灵丹抗K562和NB4细胞作用及黄酮成分研究[J].西部中医药,2018,31(07):30-33.
　WEI Xiaochen,YAN Biao,XU Naiyu,et al.Study on the Ingredients of Flavone and the Inhibiting Effects of ChouLingDan on K562 Cells and NB4 Cells[J].Western Journal of Traditional Chinese Medicine,2018,31(07):30-33.
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臭灵丹抗K562和NB4细胞作用及黄酮成分研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年07期
页码:
30-33
栏目:
出版日期:
2018-07-15

文章信息/Info

Title:
Study on the Ingredients of Flavone and the Inhibiting Effects of ChouLingDan on K562 Cells and NB4 Cells
文章编号:
1004-6852(2018)07-0030-04
作者:
韦孝晨，严彪，徐乃玉，李启润，张健△
苏州大学药学院，江苏 苏州 215123
Author(s):
WEI Xiaochen, YAN Biao, XU Naiyu, LI Qirun, ZHANG Jian△
College of Pharmacy, Suzhou University, Suzhou 215123, China
关键词:
K562细胞; NB4细胞; 黄酮; 臭灵丹
Keywords:
K562 cells;  NB4 cells;  flavone;  ChouLingDan
分类号:
R562
文献标志码:
A
摘要:
目的：研究臭灵丹对人白血病K562和NB4细胞的抑制作用及其黄酮类成分。方法：应用四甲基偶氮噻唑蓝比色法(MTT法)测臭灵丹对K562和NB4细胞生长的抑制率，采用PI/Annexin-V双染色方法考察臭灵丹对K562和NB4的凋亡作用；运用大孔树脂、Sephadex LH-20柱色谱进行化学成分的分离纯化，通过波谱分析鉴定化合物结构。结果：臭灵丹对K562和NB4细胞的抑制作用呈现剂量依赖性；臭灵丹能剂量依赖性地诱导K562和NB4细胞的凋亡；从臭灵丹中分离得到了6个黄酮类化合物，分别鉴定为洋艾素、金腰乙素、5-羟基-3'，4'，7-三甲氧基黄酮、雷杜辛黄酮醇、芹菜素-7-O-β-D-葡糖糖苷、槲皮素。结论：臭灵丹具有抗K562和NB4细胞的作用，首次从臭灵丹中分离得到雷杜辛黄酮醇。
Abstract:
Objective: To study the inhibitory effects of ChouLingDan (Laggera pterodonta) on human leukemia K562 and NB4 cells and its flavone ingredients. Methods: MTT method was used to detect the inhibitory rate of ChouLingDan on the growth of K562 and NB4 cells, PI/Annexin-V double staining method was adopted to investigate the apoptosis effects of ChouLingDan on K562 and NB4 cells; macroporous resin and Sephadex LH-20 column chromatography were adopted to seperate and purify chemical ingredients, and spectrum was applied to analyze and identify the structure of chemical compound. Results: The inhibitory effects of ChouLingDan on K562 and NB4 cells showed in a dose-dependent manner; ChouLingDan could induce the apoptosis of K562 and NB4 cells in a dose-dependent manner; six kinds of flavanoids were separated from ChouLingDan, and they were identified as absinthin, chrysosptertin B,5-hydroxy-3', 4', 7-trimethoxyflavone, retusine, apigemin-7-O-β-D-glucopyranoside and quercetin. Conclusion: ChouLingDan could inhibit K562 and NB4 cells, and retusine is isolated from the plant initially.

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备注/Memo

备注/Memo:
收稿日期：2017-02-09 *基金项目：苏州市2015年科技发展计划(编号SYS201522)。 作者简介：韦孝晨(1992—)，男，在读硕士研究生。研究方向：天然药物活性成分研究。 △通讯作者：张健(1975—)，女，硕士研究生导师，博士学位，副教授。研究方向：中药及复方药效物质研究。
更新日期/Last Update: 2018-07-15