[1]白婧,肖会敏△,何悦,等.沙菊茶HPLC指纹图谱及7个成分溶出度的研究[J].西部中医药,2018,31(11):26-31.
　BAI Jing,XIAO Huimin,HE Yue,et al.Study on HPLC Fingerprint and Dissolution Rate of Seven Kinds of Ingredients of ShaJu Tea[J].Western Journal of Traditional Chinese Medicine,2018,31(11):26-31.
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沙菊茶HPLC指纹图谱及7个成分溶出度的研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年11期
页码:
26-31
栏目:
出版日期:
2018-11-15

文章信息/Info

Title:
Study on HPLC Fingerprint and Dissolution Rate of Seven Kinds of Ingredients of ShaJu Tea
文章编号:
1004-6852(2018)11-0026-06
作者:
白婧1，肖会敏2△，何悦2，雷美娜2，张明科3，王四旺2
1 西安医学院第一附属医院神经内科，陕西 西安 710077； 2 空军军医大学药物研究所； 3 西安乐健生物科技有限公司
Author(s):
BAI Jing1, XIAO Huimin2△, HE Yue2, LEI Meina2, ZHANG Mingke3, WANG Siwang2
1 Department of Neurology, The First Affiliated Hospital of Xi′an Medical University, Xi′an 710077, China; 2 Institute of Materia Medica, the Forth Military Medical University 3 Xi′an LeJian Bio-technology Co., Ltd.
关键词:
沙菊茶; 高效液相色谱; 指纹图谱; 葛根素; 西红花苷
Keywords:
Shaju tea;  HPLC;  fingerprint;  puerarin;  crocin
分类号:
R927
文献标志码:
A
摘要:
目的：建立沙菊茶水溶性成分的高效液相色谱(HPLC)指纹图谱，并测定绿原酸、表儿茶素、葛根素、木犀草苷、木犀草素、西红花苷Ⅰ和西红花苷Ⅱ等7个成分的含量及不同泡茶时间的溶出度。方法：采用HPLC法，色谱条件：色谱柱为Intersil C18柱(250 mm×4.6 mm，5 μm)；流动相为乙腈-0.085%磷酸水溶液，使用梯度洗脱法；流速为1.0 mL/min；检测波长为270 nm和440 nm；柱温为40℃。结果：1)通过中药色谱指纹图谱相似度评价系统(2004A)确定沙菊茶水溶性成分的HPLC指纹图谱共有模式，标定40个共有峰，相似度为0.952～0.993；2)10批制剂中绿原酸、表儿茶素、葛根素、木犀草苷、木犀草素、西红花苷Ⅰ和西红花苷Ⅱ的含量(mg/g)分别为0.1717、0.3726、0.8735、0.0282、0.5314、0.5652和0.1275 mg/g，RSD分别为1.44%、0.83%、0.760%、1.07%、0.81%、0.91%和1.88 %；3)茶中7个成分的含量随浸泡时间的延长逐渐升高，在60 分钟后均能达到峰值。结论：HPLC指纹图谱方法与7个成分的含量检测方法以及溶出度测定方法简便、稳定、科学，可用于沙菊茶的质量控制并指导患者正确使用。
Abstract:
Objective: To establish a HPLC fingerprint of aqueous components of Shaju tea, and detect the contents of seven kinds of ingredients including chlorogenic acid, epicatechin, puerarin, galuteolin, luteolin, crocinⅠand crocinⅡ in the preparations and the dissolution rates of these ingredients in different soaking time. Methods: The high performance liquid chromatography (HPLC) analysis was performed on such chromatographic conditions: chromatographic column was Intersil C18(250 mm×4.6 mm, 5 μm), the mobile phase was a mixture of acetonitrile and 0.085 % phosphoric acid solution in gradient elution; the flow rate was 1.0 mL/min, and the detection wavelength was 270 nm and 440 nm, the column temperature was set at 40℃. Results:1) Under the above chromatographic conditions, HPLC fingerprint common modes of aqueous components of Shaju tea were confirmed by chromatographic fingerprint similarity evaluation system(2004A), and 40 chromatographic peaks were composed, and the overall similarity degree was from 0.952 to 0.993; 2) The average contents of chlorogenic acid, epicatechin, puerarin, galuteolin, luteolin, crocinⅠand crocinⅡ were 0.1717, 0.3726, 0.8735, 0.0282, 0.5314, 0.565 2 and 0.127 5 mg/g in ten batches of Shaju tea, RSDs were 1.44%, 0.83%, 0.760%, 1.07%, 0.81%, 0.91% and 1.88%; 3) The contents of seven kinds of ingredients raised gradually as the soaking time prolonged, and it will reach the peak value in 60 minutes. Conclusion: The method of HPLC fingerprint, the method of detecting the contents of seven kinds of ingredients and the detection method of dissolution rate are simple, stable and scientific, and they could be used for quality control of Shaju tea and to guide the patients' right use.

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备注/Memo

备注/Memo:
收稿日期：2018-02-20 *基金项目：陕西省科学技术研究发展计划项目(编号 2015SF2-08-01)。 作者简介：白婧(1982—)，女，硕士学位，主治医师。研究方向：脑血管疾病。 △通讯作者：肖会敏(1975—)，男，博士学位，副研究员。研究方向：天然药物的新药研究。
更新日期/Last Update: 2018-11-15