[1]李伟,王加志△,曲岩,等.马钱苷调节UVB诱导的表皮黑素单位黑素生成的实验研究[J].西部中医药,2019,32(02):13-16.
　LI Wei,WANG Jiazhi,QU Yan,et al.Experimental Study on Loganin Regulating the Generation of Melanin in UVB-induced Epidermal Melanin Unit[J].Western Journal of Traditional Chinese Medicine,2019,32(02):13-16.
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马钱苷调节UVB诱导的表皮黑素单位黑素生成的实验研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年02期
页码:
13-16
栏目:
出版日期:
2019-02-15

文章信息/Info

Title:
Experimental Study on Loganin Regulating the Generation of Melanin in UVB-induced Epidermal Melanin Unit
文章编号:
1004-6852(2019)02-0013-04
作者:
李伟，王加志△，曲岩，王兴焱，陈景华，党文军
黑龙江中医药大学佳木斯学院，黑龙江 佳木斯 154007
Author(s):
LI Wei, WANG Jiazhi△, QU Yan, WANG Xingyan, CHEN Jinghua, DANG Wenjun
Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi 154007, China
关键词:
马钱苷; 酪氨酸酶; 表皮黑素单位; P38MAPK
Keywords:
loganin;  tyrosinase;  epidermal melanin unit;  P38MAPK
分类号:
R285
摘要:
目的：探讨不同浓度马钱苷对中波紫外线(UVB)诱导该模型中黑素生成及其作用的影响。方法：常规体外共培养人黑色素瘤A375、人皮肤角质形成细胞(HaCaT)，以UVB20 mJ/cm2照射后加含马钱苷的培养液作用24 h，四甲基偶氮唑蓝(MTT)比色法测定细胞增殖；采用多巴氧化法研究酪氨酸酶活性的变化；比色法测定黑素含量，免疫印迹法测定P38通路蛋白表达。结果：马钱苷0.01 μm/L可抑制UVB诱导的表皮黑素单位中酪氨酸酶活性增高，减少黑素生成，其机制可能是通过抑制P38蛋白表达减弱信号通路作用，抑制酪氨酸酶活性而实现的。结论：高浓度马钱苷可明显抑制P38MAPK通路，进而抑制酪氨酸酶活性和黑素生成。
Abstract:
Objective：To discuss the effects of different concentrations of loganin on the generation of melanin in UVB-induced epidermal melanin unit and the mechanism. Methods: A375 and Hacat cells were co-cultured in vitro as routine, after radiated with UVB 20 mJ/cm2 and adding nutrient solution containing loganin for 24 hours, cellular proliferation was detected using MTT chromatometry; the activity changes of tyrosinase were studied using Dopa oxidation method; the contents of melanin were detected using chromatometry, and the expressions of P38 pathway protein were measured using immunoblotting. Results: Loganin in the concentrations of 0.01μm/L could inhibit the activity increase of tyrosinase in UVB-induced epidermal melanin unit and reduce the generation of melanin, and the mechanism might be realized by inhibiting the expressions of P38 protein, reducing signaling pathway and inhibiting the activity of tyrosinase. Conclusion: High concentrations of loganin could obviously inhibit P38MAPK pathway, therefore to inhibit the activity of tyrosinase and the generation of melanin.

相似文献/References:

[1]李富欣,于蓓蓓,刘瑾,等.茯黄颗粒的质量标准研究[J].西部中医药,2019,32(02):44.
　LI Fuxin,YU Beibei,LIU Jin,et al.Study on Quality Standard of FuHuang Granules[J].Western Journal of Traditional Chinese Medicine,2019,32(02):44.

备注/Memo

备注/Memo:
收稿日期：2018-07-04 *基金项目：黑龙江省自然科学基金面上项目(编号H2015039)；黑龙江省中医药管理局中青年科技攻关项目(编号ZQG-018)；黑龙江中医药大学校基金(编号201317)；黑龙江中医药大学佳木斯学院院基金(编号JYKY201504)。 作者简介：李伟(1979—)，女，硕士学位，讲师。研究方向：美容药物学。 △通讯作者：王加志(1977—)，男，博士学位，副教授。研究方向：中药药理学。
更新日期/Last Update: 2019-02-15