[1]闫向勇,李俊,蔺彩娟,等.四君子汤合膈下逐瘀汤对人肝癌细胞株HepG2和SMMC体外作用的实验研究[J].西部中医药,2019,32(04):12-15.
　YAN Xiangyong,LI Jun,LIN Caijuan,et al.Experimental Study of In-vitro Effects of SiJunZi Decoction Joined with GeXia ZhuYu Decoction on Human Liver Cell Line HepG2 and SMMC[J].Western Journal of Traditional Chinese Medicine,2019,32(04):12-15.
点击复制

四君子汤合膈下逐瘀汤对人肝癌细胞株HepG2和SMMC体外作用的实验研究
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年04期
页码:
12-15
栏目:
出版日期:
2019-04-15

文章信息/Info

Title:
Experimental Study of In-vitro Effects of SiJunZi Decoction Joined with GeXia ZhuYu Decoction on Human Liver Cell Line HepG2 and SMMC
文章编号:
1004-6852(2019)04-0012-04
作者:
闫向勇，李俊，蔺彩娟，宋书征，燕忠生△
中国人民解放军联勤保障部队第940医院，甘肃 兰州 730050
Author(s):
YAN Xiangyong, LI Jun, LIN Caijuan, SONG Shuzheng, YAN Zhongsheng△
TCM Department, Hospital 940 of PLA Joint Logistics Support Force, Lanzhou 730050, China
关键词:
四君子汤; 膈下逐瘀汤; 人肝癌细胞株
Keywords:
SiJunZi decoction;  GeXia ZhuYu decoction;  human liver cancer cell line
分类号:
R273
文献标志码:
A
摘要:
目的：观察四君子汤合膈下逐瘀汤对人肝癌细胞HepG2和SMMC-7721的影响。方法：倒置显微镜观察中药组、阴性对照组、氟尿嘧啶组中HePG2及SMMC-7721细胞生长情况。采用MTT法检测3组细胞生长的抑制率，流式细胞仪(FCM)检测人肝癌HepG2和SMMC-7721细胞凋亡情况及细胞周期。结果：倒置显微镜下观察阳性对照组及中药组中人肝癌HepG2和SMMC-7721细胞生长明显受抑制，MTT法测定细胞增殖抑制率。中药对HepG2和SMMC-7721细胞的增殖均有明显的抑制作用，与阴性对照组比较差异有统计学意义(P＜0.05)。FCM检测中药对HepG2、SMMC-7721细胞凋亡具有一定的诱导作用，与对照组比较差异有统计学意义(P＜0.05)。结论：四君子汤合膈下逐瘀汤具有抑制人肝癌HepG2和SMMC-7721细胞增殖，促进其凋亡的作用。
Abstract:
Objective：To observe the effects of SiJunZi decoction combined with GeXia ZhuYu decoction on human liver cell line HepG2 and SMMC-7721. Methods: Inverted microscope was used to observe the growth conditions of SMMC-7721 and HePG2 in herbal group, negative control group and fluorouracil group. MTT method was adopted to detect the inhibitory rate of cellular growth in three groups, flow cytometry (FCM) was used to detect the apoptosis conditions of human liver cell HepG2 and SMMC-7721 and cell cycles. Results: The growth of human liver cell line HepG2 and SMMC-7721 cells were obviously inhibited in the positive control group and herbal group under the inverted microscope, MTT method was used to detect the inhibitory rate of cellular proliferation, herbs showed the notable inhibitory effects on the proliferation of HepG2 and SMMC-7721 cells, there was the significant difference when compared with negative control group(P＜0.05). FCM detection demonstrated that herbs could induce cellular apoptosis of HepG2 and SMMC-7721 to a certain degree, there was the significant difference when compared with the control group (P＜0.05). Conclusion: SiJunZi decoction combined with GeXia ZhuYu decoction could inhibit the proliferation of human liver cell line HepG2 and SMMC-7721 and induce their apoptosis.

相似文献/References:

[1]葛韵芝,许寅,丁超.基于网络药理学分析四君子汤治疗结直肠癌的作用机制[J].西部中医药,2021,34(08):84.[doi:10.12174/j.issn.2096-9600.2021.08.18]
　GE Yunzhi,XU Yin,DING Chao.Research on the Mechanism of Sijunzi Decoction in the Treatment of Colorectal Cancer Based on Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2021,34(04):84.[doi:10.12174/j.issn.2096-9600.2021.08.18]
[2]陈成,付越,李亮,等.基于Wnt/β-catenin信号通路探讨四君子汤对胃癌脾气虚证裸鼠的作用机制[J].西部中医药,2023,36(09):40.[doi:10.12174/j.issn.2096-9600.2023.09.08]
　CHEN Cheng,FU Yue,LI Liang,et al.The Mechanism of Sijunzi Tang in the Treatment of Gastric Cancer of Spleen Qi Deficiency Pattern in Nude Mice Based on Wnt/β-catenin Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2023,36(04):40.[doi:10.12174/j.issn.2096-9600.2023.09.08]
[3]陈小霞,陈国忠,谢彩杏,等.基于网络药理学和分子对接分析古方四君子汤辅助治疗胃癌的作用机制[J].西部中医药,2024,37(04):72.[doi:10.12174/j.issn.2096-9600.2024.04.15]
　CHEN Xiaoxia,CHEN Guozhong,XIE Caixing,et al.Analysis of the Mechanism of Ancient Formula Sijunzi Tang in the Adjuvant Treatment of Gastric Cancer Based on Network Pharmacology and Molecular Docking[J].Western Journal of Traditional Chinese Medicine,2024,37(04):72.[doi:10.12174/j.issn.2096-9600.2024.04.15]

备注/Memo

备注/Memo:
收稿日期：2018-09-10 *基金项目：兰州市科技计划项目(编号2018-3-60)。 作者简介：闫向勇(1980—)，男，主治医师。研究方向：肿瘤疾病的诊治。 △通讯作者：燕忠生(1968—)，男，硕士学位,主任医师。研究方向：肿瘤疾病的诊治。
更新日期/Last Update: 2019-04-15