[1]杨晓露,陈文莉△,陈红宇,等.扶正抑瘤方醇提物对人肺癌A549细胞增殖和调亡的影响[J].西部中医药,2019,32(07):12-15.
　YANG Xiaolu,CHEN Wenli,CHEN Hongyu,et al.Influence of Alcohol Extract from Healthy Qi- enhancing Tumor-inhibiting Prescription on the Proliferation and Apoptosis of Human Pulmonary Cancer A549 Cell[J].Western Journal of Traditional Chinese Medicine,2019,32(07):12-15.
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扶正抑瘤方醇提物对人肺癌A549细胞增殖和调亡的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年07期
页码:
12-15
栏目:
出版日期:
2019-07-15

文章信息/Info

Title:
Influence of Alcohol Extract from Healthy Qi- enhancing Tumor-inhibiting Prescription on the Proliferation and Apoptosis of Human Pulmonary Cancer A549 Cell
文章编号:
1004-6852(2019)07-0012-04
作者:
杨晓露，陈文莉△，陈红宇，史勤，丁明明，夏云
上海市宝山区中西医结合医院，上海 201999
Author(s):
YANG Xiaolu, CHEN Wenli△, CHEN Hongyu, SHI Qin, DING Mingming, XIA Yun
Baoshan Branch of Shanghai Municipality Hospital of Integrative Medicine, Shanghai 201999, China
关键词:
人肺癌A549细胞; 增殖; 凋亡; 扶正抑瘤方醇提物
Keywords:
human pulmonary cancer A549 cells;  proliferation;  apoptosis;  alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨扶正抑瘤方醇提物对人肺癌A549细胞增殖抑制和诱导调亡的作用。方法：将人肺癌A549细胞在体外加入不同浓度的扶正抑瘤方醇提物72小时后，采用CCK8法检测该方的增殖抑制作用，以Annexin V-EGFP标记法,通过流式细胞术检测该方对人肺癌A549细胞凋亡的影响。结果：扶正抑瘤方醇提物能抑制人肺癌A549细胞的增殖，与浓度相关，与空白对照组比较有明显差异(P＜0.05)；显微镜下可见到明显的凋亡细胞，流式细胞检测结果提示随着浓度的增加，细胞凋亡率增加。结论：扶正抑瘤方醇提物能显著抑制人肺癌A549细胞增殖，可能与诱导细胞凋亡有关。
Abstract:
Objective: To explore the effects of alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription on inhibiting the proliferation and inducing the apoptosis of human pulmonary cancer A549 cells. Methods: After adding different concentrations of alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription to human pulmonary cancer A549 cells in vitro for 72 hours, the inhibitory effects on the proliferation was detected using CCK8 method, the effects of the prescription on the apoptosis of human pulmonary cancer A549 cells were detected using flow cytometry. Results: Alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription could inhibit the proliferation of human pulmonary cancer A549 cells, there was the obvious difference compared with blank control group(P＜0.05); the notable apoptotic cells could be seen under the microscope, the results of flow cytometry showed that cellular apoptosis rate upped as the concentrations increased. Conclusion: Alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription could obviously inhibit the proliferation of human pulmonary cancer A549 cells, which might be related to inducing cellular apoptosis.

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备注/Memo

备注/Memo:
收稿日期：2018-06-26*基金项目：上海市宝山区科学技术委员会科技创新专项资金项目(编号16-E-15)。作者简介：杨晓露(1986—)，女，硕士学位，主管中药师。研究方向：恶性肿瘤的中医药防治。△通讯作者：陈文莉(1971—)，女，硕士研究生导师，博士学位，副主任医师。研究方向：肿瘤疾病的中医诊治。
更新日期/Last Update: 2019-07-15