[1]夏嘉,王颖,刘铭洁,等.抗纤灵对慢性肾功能衰竭模型Smad2-KO小鼠心脏CoI、CoIII、AngII、TNF-α、IL-6的影响[J].西部中医药,2021,34(02):1-5.[doi:10.12174/j.issn.2096-9600.2021.02.04]
　XIA Jia,WANG Ying,LIU Mingjie,et al.Influence of Kangxianling on the Expressions of CoI, CoIII, AngII, TNF-α and IL-6 in the Heart of Smad2-KO Mice of CRF Model[J].Western Journal of Traditional Chinese Medicine,2021,34(02):1-5.[doi:10.12174/j.issn.2096-9600.2021.02.04]
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抗纤灵对慢性肾功能衰竭模型Smad2-KO小鼠心脏CoI、CoIII、AngII、TNF-α、IL-6的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年02期
页码:
1-5
栏目:
出版日期:
2021-02-15

文章信息/Info

Title:
Influence of Kangxianling on the Expressions of CoI, CoIII, AngII, TNF-α and IL-6 in the Heart of Smad2-KO Mice of CRF Model
作者:
夏嘉1, 王颖2, 刘铭洁3, 高雅婵4, 陈文浩4, 何立群4
1.上海中医药大学附属市中医医院，上海 200071
2.上海中医药大学附属中西医结合医院
3.贵阳中医学院第一附属医院
4.上海中医药大学附属曙光医院
Author(s):
XIA Jia1, WANG Ying2, LIU Mingjie3, GAO Yachan4, CHEN Wenhao4, HE Liqun4
1.Shanghai City Hospital of Traditional Chinese Medicine, Shanghai 200071, China
2.Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM
3.The Affiliated Hospital to Guizhou University of Traditional Chinese Medicine
4.Shuguang Hospital Affiliated to Shanghai University of TCM
关键词:
抗纤灵; Smad2基因敲除; 慢性肾衰; 心脏; 动物实验
Keywords:
Smad2 gene knockout; CRF; heart; animal experiment
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2021.02.04
摘要:
目的观察抗纤灵对慢性肾功能衰竭（chronic renal failure，CRF）模型Smad2-KO小鼠心脏I型胶原（collagen I，CoI）、Ⅲ型胶原（collagen Ⅲ，CoⅢ）、血管紧张素Ⅱ（Angiotensin Ⅱ，AngⅡ）、肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）、白细胞介素6（Interleukin-6，IL-6）水平的影响，探讨抗纤灵治疗CRF的作用机制。 方法将18只假手术Smad2-KO小鼠随机分为假手术组（蒸馏水0.5 mL，灌胃）、抗纤灵假手术组（抗纤灵复方20 g/kg，0.5 mL灌胃）、缬沙坦假手术组（缬沙坦20 mg/kg，0.5 mL灌胃），每组6只。将24只CRF模型Smad2-KO小鼠随机分为模型组（蒸馏水0.5 mL，灌胃）、抗纤灵模型组（抗纤灵复方20 g/kg，0.5 mL灌胃）、缬沙坦模型组（缬沙坦20 mg/kg，0.5 mL灌胃），每组8只。灌胃8周后取材，Elisa法检测心脏AngⅡ的水平，Western blot法测心脏CoI、CoⅢ、TNF-α、IL-6的相对表达。 结果与假手术组比较，模型组的AngⅡ、CoⅠ、CoⅢ、TNF-α、IL-6均显著增高（P＜0.01），缬沙坦假手术组的AngⅡ下降（P＜0.05）。与模型组比较，抗纤灵模型组的AngⅡ有改善（P＜0.05），CoI、CoⅢ、TNF-α、IL-6有显著改善（P＜0.01）。缬沙坦模型组的CoI、CoⅢ、AngⅡ、TNF-α、IL-6均明显改善（P＜0.01）。抗纤灵模型组与缬沙坦模型组组间比较，缬沙坦在改善AngⅡ方面优于抗纤灵组（P＜0.05），其余指标两组间比较差异无统计学意义（P＞0.05）。 结论抗纤灵可改善CRF模型Smad2-KO小鼠心脏病变的作用机制可能与降低炎症因子的表达、调节RAS和减少心脏胶原的过度沉积有关。
Abstract:
ObjectiveTo observe the influence of Kangxianling on the levels of CoI, CoIII, AngII, TNF-α and IL-6 in the heart of Smad2-KO mice of CRF model, and to explore its mechanism of treating CRF. MethodsEighteen Smad2-KO mice were randomized into sham operation group (distilled water, 0.5mL, intragastric administration), Kangxianling sham operation group (Kangxianling compound, 20g/kg, 0.5mL, intragastric administration) and Valsartan sham operation group (Valsartan, 20mg/kg, 0.5mL, intragastric administration), six mice each group. 24 Smad2-KO mice of CRF model were allocated to the model group (distilled water, 0.5mL, intragastric administration), Kangxianling model group (Kangxianling compound, 20g/kg, 0.5mL, intragastric administration) and Valsartan model group (Valsartan, 20mg/kg, 0.5mL, intragastric administration), eight mice each group. After drenched for eight weeks, Elisa method was used to detect the levels of cardiac AngII, Western blot method to detect the relative expressions of CoI, CoIII, TNF-α and IL-6 in the mouse heart. ResultsCompared with sham operation group, the expressions of AngII, CoI, CoIII, TNF-α and IL-6 increased remarkably in the model group (P<0.01), the levels of AngII lowered in Valsartan sham operation group (P<0.05). Compared with the model group, the levels of AngII of Kangxianling model group were improved (P<0.05), the expressions of CoI, CoIII, TNF-α and IL-6 were improved notably (P<0.01). The expressions of CoI, CoIII, AngII, TNF-α and IL-6 were improved notably in Valsartan model group (P<0.01). When Kangxianling model group was compared with Valsartan model group, Valsartan was better than Kangxianling in improving AngII (P<0.05), there was no significant difference between two groups in other indexes (P>0.05). ConclusionThe mechanism of Kangxianling improving cardiac abnormalities of Smad2-KO mice of CRF model might be related to lowering the expression of inflammatory factors, regulating RAS and reducing excessive deposition of cardiac collagen.

备注/Memo

备注/Memo:
夏嘉（1981—），女，博士学位，副主任医师。研究方向：肾脏及风湿免疫疾病的中西医结合诊治。上海市卫生健康委员会科研课题（201940327）；上海市名老中医学术经验研究工作室资助项目（SHGZS-2017027）。
更新日期/Last Update: 2021-02-15