蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的影响-《西部中医药》

文章信息/Info

Title:: Effects of Different Parts of Dandelion Water Extract on Human Osteosarcoma MG-63 Cells

作者:: 徐世红^1,2, 柳博³, 谢兴文⁴, 李鼎鹏^1,2, 许伟², 丁聚贤³, 黄睿³, 姜朝阳³; 1.甘肃省中医药研究院，甘肃兰州 730050
2.甘肃省中医院
3.甘肃中医药大学
4.甘肃省第二人民医院

Author(s):: XU Shihong^1,2, LIU Bo³, XIE Xingwen⁴, LI Dingpeng^1,2, XU Wei², DING Juxian³, HUANG Rui³, JIANG Chaoyang³; 1.Gansu Provincial Academy of Chinese Medicine, Lanzhou 730050, China
2.Gansu Provincial Hospital of Traditional Chinese Medicine
3.Gansu University of Chinese Medicine
4.Second Provincial People's Hospital of Gansu

摘要:: 目的探讨蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的细胞形态及增殖的影响。方法将蒲公英加水煎煮得蒲公英水提液，然后用石油醚、乙酸乙酯和正丁醇依次以1∶1比例萃取蒲公英水提物，得到不同溶剂萃取部位；人骨肉瘤MG-63细胞传代贴壁培养后应用蒲公英水提液不同萃取部位培养；采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium，MTT)法检测蒲公英水提液各萃取部位在不同时间内对人骨肉瘤细胞MG-63的增殖抑制作用。结果蒲公英水提液各萃取部位在体外对MG-63细胞增殖具有抑制作用，呈现出剂量与时间的效应关系。结论蒲公英水提液各萃取部位能抑制人骨肉瘤MG-63细胞，在体外有一定抗肿瘤活性，其中以乙酸乙酯萃取部位最有效。

Abstract:: ObjectiveTo explore the effects of different parts of dandelion water extract on the cellular morphology and proliferation of human osteosarcoma(OS) MG-63 cells. MethodsWater extract of dandelion was obtained by decocting the whole dandelion with water; different solvent extracts were got by extracting dandelion water extract with petroleum ether, ethyl acetate and n-butanol in the ratio of 1:1, different extracts of dandelion were applied to culture human osteosarcoma MG-63 cells after passage and adherent culture; MTT method was adopted to detect the inhibitory effects of dandelion water extract on the proliferation of human osteosarcoma MG-63 cells at different times. ResultsDandelion water extract could inhibit the proliferation of MG-63 cells in vitro, presenting in the dose-time concentration-effect relationship. ConclusionDandelion water extract could inhibit human osteosarcoma MG-63 cells, it has certain anti-tumor activity in vitro, and dandelion ethyl acetate extract is particularly the most effective.