[1]张斌,秦永,范道波,等.基于PI3K/Akt信号通路探讨益气温阳活血方对老年肾虚血瘀型骨质疏松患者骨代谢的影响[J].西部中医药,2022,35(07):68-71.[doi:10.12174/j.issn.2096-9600.2022.07.18]
　ZHANG Bin,QIN Yong,FAN Daobo,et al.Effects of Benefiting-Qi Warming-Yang Activating-blood Prescription on Bone Metabolism in Senile Patients with Renal Deficiency and Blood Stasis Pattern Osteoporosis Based on P13K/Akt Signal Pathway[J].Western Journal of Traditional Chinese Medicine,2022,35(07):68-71.[doi:10.12174/j.issn.2096-9600.2022.07.18]
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基于PI3K/Akt信号通路探讨益气温阳活血方对老年肾虚血瘀型骨质疏松患者骨代谢的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年07期
页码:
68-71
栏目:
出版日期:
2022-07-15

文章信息/Info

Title:
Effects of Benefiting-Qi Warming-Yang Activating-blood Prescription on Bone Metabolism in Senile Patients with Renal Deficiency and Blood Stasis Pattern Osteoporosis Based on P13K/Akt Signal Pathway
作者:
张斌1, 秦永1, 范道波1, 李中连1, 冯冬前1, 刘立1, 李兰亚1, 葛大伟2
1.沭阳县中医院骨伤科，江苏 沭阳 223600
2.南京医科大学附属南京医院骨科
Author(s):
ZHANG Bin1, QIN Yong1, FAN Daobo1, LI Zhonglian1, FENG Dongqian1, LIU Li1, LI Lanya1, GE Dawei2
1.Orthopedics Department, Shuyang County TCM Hospital, Shuyang 223600, China
2.Orthopedics, Nanjing Hospital Affiliated to Nanjing Medical University
关键词:
骨质疏松症; 肾虚血瘀; 骨密度; 骨代谢; PI3K/AKT通路; 益气温阳活血方
Keywords:
osteoporosis; renal deficiency and blood stasis pattern; bone mineral density; bone metabolism; P13K/AKT pathway; benefiting- warming- activating-blood prescription
分类号:
R274
DOI:
10.12174/j.issn.2096-9600.2022.07.18
文献标志码:
B
摘要:
目的基于PI3K/Akt信号通路探讨益气温阳活血方对老年肾虚血瘀型骨质疏松患者骨代谢的影响。 方法将老年肾虚血瘀型骨质疏松患者100例随机分为观察组与对照组各50例，对照组予钙尔奇+阿法骨化醇治疗，观察组在对照组治疗的基础上联合益气温阳活血方治疗。治疗前及治疗3个月后，采用双能X线仪检测骨密度（bone mineral density，BMD），酶联免疫吸附法检测骨代谢指标I型胶原羧基端肽β特殊序列（β-terminal cross-linked telopeptides of type Ⅰ collagen，β-CTX）及I型前胶原氨基端延长肽（propeptide of type I NT-procollagen，PINP）含量，Western blot法检测外周血单个核细胞中磷酸化PI3K（phosphorylation PI3K，p-PI3K）、磷酸化AKT（phosphorylation AKT，p-AKT）表达量；观察治疗期间不良事件。 结果治疗后两组患者BMD较治疗前升高，观察组高于对照组（P＜0.05）；治疗后观察组血清β-CTX含量较治疗前及治疗后对照组降低、PINP含量较治疗前及对照组治疗后升高（P＜0.05）；治疗后观察组外周血p-PI3K、p-AKT表达量较治疗前及对照组治疗后升高（P＜0.05），对照组治疗前后外周血p-PI3K、p-AKT表达量比较差异无统计学意义（P＞0.05）。 结论益气温阳活血方联合钙尔奇、阿法骨化醇能够增加老年肾虚血瘀型骨质疏松患者骨密度，改善骨代谢，机制可能与激活PI3K/AKT通路有关。
Abstract:
ObjectiveTo explore the effects of benefiting-Qi warming-Yang activating-blood prescription on bone metabolism in senile patients with renal deficiency and blood stasis pattern osteoporosis on the foundation of P13K/Akt signal pathway. MethodsAll 100 patients were randomized into the observation group and the control group with 50 cases in each group. The control group was treated by caltrate+alfacalcidol, and the observation group took benefiting-Qi warming-Yang activating-blood prescription orally based on the therapy the control group received. BMD was measured by dual-energy X-ray apparatus before treatment and after three months of treatment, the contents of bone metabolism markers β-CTX and PINP were detected by ELISA method, the expressions of p-PI3K and p-AKT of mononuclear cell in peripheral blood were tested by Western blot, to observe the adverse events during therapeutic period. ResultsBMD increased in both groups after the treatment compared with these before treatment, and the observation group was higher than the control group (P<0.05), the contents of serum β-CTX lowered than before treatment, and the control group after the treatment, the contents of PINP raised compared with before treatment and the control group after the therapy (P<0.05); after the therapy, the expressions of p-PI3K and p-AKT in peripheral blood rose in the observation group compared with these before the treatment and the control group after the treatment (P<0.05), the difference had no statistical meaning in the expressions of p-PI3K and p-AKT in peripheral blood before and after treatment in the control group (P>0.05). ConclusionBenefiting-Qi warming-Yang activating-blood prescription combined with caltrate and alfacalcidol could increase BMD and improve bone metabolism in senile patients with renal deficiency and blood stasis pattern osteoporosis, and its mechanism might be related to P13K/AKT pathway activation.

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备注/Memo

备注/Memo:
张斌（1977—），男，主任医师。研究方向：脊柱外科、骨质疏松性骨折的基础及临床研究。
更新日期/Last Update: 2022-07-15