[1]姚欢,李维凤,牛晓峰.HPLC测定佛甲草中δ-香树酯酮的含量[J].西部中医药,2022,35(12):77-79.[doi:10.12174/j.issn.2096-9600.2022.12.16]
　YAO Huan,LI Weifeng,NIU Xiaofeng.Contents of δ-amyrone Contained in Fojiacao Determined by HPLC[J].Western Journal of Traditional Chinese Medicine,2022,35(12):77-79.[doi:10.12174/j.issn.2096-9600.2022.12.16]
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HPLC测定佛甲草中δ-香树酯酮的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年12期
页码:
77-79
栏目:
出版日期:
2022-12-15

文章信息/Info

Title:
Contents of δ-amyrone Contained in Fojiacao Determined by HPLC
作者:
姚欢1, 李维凤2, 牛晓峰2
1.咸阳市中心医院，陕西 咸阳 712000
2.西安交通大学药学院
Author(s):
YAO Huan1, LI Weifeng2, NIU Xiaofeng2
1.Xianyang Central Hospital, Xianyang 712000, China
2.Pharmacy School of Xi'an Jiaotong University
关键词:
高效液相色谱; 佛甲草; δ-香树酯酮; 含量测定
Keywords:
HPLC; δ-amyrone; content determination
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2022.12.16
文献标志码:
A
摘要:
目的建立用高效液相色谱法（high performance liquid chromatography，HPLC）测定佛甲草中δ-香树酯酮含量的方法。 方法采用Agilent ZORBAX Eclipse XDB-C8色谱柱（150×4.6 mm，5 μm），流动相为乙腈-水-磷酸-三乙胺（85∶15∶0.1∶0.1，v/v）；柱温为25 ℃，流速为1.0 mL/min，检测波长为211 nm。δ-香树酯酮在浓度为0.5～40.5 μg/mL范围内峰面积与浓度呈良好的线性关系，相关系数为R2=0.9991，平均回收率为98.7%。 结果δ-香树酯酮在此色谱条件下的保留时间为26.15 min，经分析计算可得，西安交通大学药学院药圃里佛甲草中δ-香树酯酮的含量为860.5 μg/100 g生药，在秦岭采集的佛甲草中δ-香树酯酮的含量为906.8 μg/100 g生药。 结论HPLC法测得的结果准确可靠，可作为δ-香树酯酮质量控制的一种方法。
Abstract:
ObjectiveTo establish a method of determining the contents of δ-amyrone contained in Fojiacao (Sedum lineare Thunb.) by HPLC. MethodsAgilent ZORBAX Eclipse XDB-C8 chromatographic column (150×4.6 mm, 5 μm), mobile phase: acetonitrile-water-phosphate -triethylamine (85∶15∶0.1∶0.1, v/v); column temperature:25 ℃, flow rate was 1.0 mL/min, detection wavelength was 211 nm. δ-amyrone presented good linearity between peak area and concentration in the range of 0.5 and 40.5 μg/mL, the correlation coefficient was R2=0.9991, average recovery rate was 98.7%. ResultsThe retention time of δ-amyrone was 26.15 min under the chromatographic conditions, by analysis and calculation, the contents of δ-amyrone were 860.5 μg/100 g crude drug contained in Fojiacao planted in the medicinal garden of pharmacy shcool, Xi'an Jiaotong University, while its contents were 906.8 μg/100 g crude drug contained in Fojiacao gathered from Qinling mountains. ConclusionThe results are accurate and reliable obtained by HPLC, and it could be taken as a approach for quality control of δ-amyrone.

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备注/Memo

备注/Memo:
姚欢（1990—），女，硕士学位，主管药师。研究方向：临床药学。陕西省自然科学基金（2014JM4116）。
更新日期/Last Update: 2022-12-15