五味子甲素对炎性肠病小鼠结肠NF-<italic>κ</italic>B/COX-2信号通路的影响-《西部中医药》

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Title:: Influence of Deoxyschizandrin on NF-κB/COX-2 Signal Pathway in Colon of Mice with Inflammatory Bowel Disease

作者:: 王绍洁¹, 张莹¹, 梁晗业², 张明波², 徐志立²; 1.大连市儿童医院，辽宁大连 116012
2.辽宁中医药大学药学院，辽宁大连 116600

Author(s):: WANG Shaojie¹, ZHANG Ying¹, LIANG Hanye², ZHANG Mingbo², XU Zhili²; 1.Dalian Children's Hospital, Dalian 116012, China
2.College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China

摘要:: 目的考察五味子甲素对三硝基苯磺酸所致炎性肠病小鼠核转录因子κB（nuclear factor-κB，NF-κB）/环氧合酶2（cyclooxygenase，COX-2）通路的抑制作用。方法采用三硝基苯磺酸灌肠诱导小鼠炎性肠病，采用ELISA、免疫组化、WB法测定小鼠体内NF-κB及COX-2表达，采用RT-PCR法测定小鼠结肠组织NF-κB及COX-2 mRNA表达，观察小鼠体质量、腹泻症状和疾病活动指数。结果炎性肠病小鼠体内NF-κB、COX-2含量、蛋白及其mRNA表达增多，五味子甲素可抑制NF-κB、COX-2 mRNA和蛋白表达，抑制炎性肠病小鼠体质量减轻和疾病活动指数，改善小鼠腹泻情况。结论五味子甲素可通过抑制NF-κB/COX-2通路的激活而抑制炎症反应，对三硝基苯磺酸诱导的炎性肠病小鼠具有治疗作用。

Abstract:: ObjectiveTo survey the inhibitory effects of deoxyschizandrin on NF-κB/COX-2 signal pathway in mice suffering TNBS-induced inflammatory bowel disease (IBD). MethodsTNBS enema was adopted to induce IBD in mice, ELISA, immunohistochemical method and WB method were applied to detect the expressions of NF-κB and COX-2 in mice, RT-PCR method was utilized to measure the expression of NF-κB and COX-2 mRNA, to observe body weight, the symptoms of diarrhea and disease activity index (DAI). ResultsThe expressions of NF-κB and COX-2 mRNA and protein increased in IBD mice, while deoxyschizandrin could inhibit the expressions of NF-κB and COX-2 mRNA and protein, restrain the reduction of body weight and DAI in IBD mice and improve diarrhea. ConclusionDeoxyschizandrin could inhibit inflammatory reaction through the inhibition of the activation of NF-κB/COX-2 signal pathway, thus showing therapeutic effects on TNBS-induced IBD in mice.