[1]罗玉清,梁文菲,张熙,等.黄芪甲苷对高糖受损间充质干细胞生物学功能和分泌HGF的影响[J].西部中医药,2023,36(11):28-32.[doi:10.12174/j.issn.2096-9600.2023.11.06]
　LUO Yuqing,LIANG Wenfei,ZHANG Xi,et al.Influence of Astragaloside IV on Biological Function and Secretion of HGF in Mesenchymal Stem Cells Damaged by High Glucose[J].Western Journal of Traditional Chinese Medicine,2023,36(11):28-32.[doi:10.12174/j.issn.2096-9600.2023.11.06]
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黄芪甲苷对高糖受损间充质干细胞生物学功能和分泌HGF的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年11期
页码:
28-32
栏目:
出版日期:
2023-11-15

文章信息/Info

Title:
Influence of Astragaloside IV on Biological Function and Secretion of HGF in Mesenchymal Stem Cells Damaged by High Glucose
作者:
罗玉清1, 梁文菲2, 张熙3, 谭梅鑫4,5, 刘锦清5, 夏艺菲5, 朱芙蓉1, 熊武1
1.湖南中医药大学第一附属医院，湖南 长沙 410007
2.广州中医药大学东莞医院，广东 东莞 523000
3.湖南省脑科医院，湖南 长沙 410007
4.湖南中医药大学临床医学院，湖南 长沙 410007
5.湖南中医药大学中西医结合学院，湖南 长沙 410208
Author(s):
LUO Yuqing1, LIANG Wenfei2, ZHANG Xi3, TAN Meixin4,5, LIUJinqing 5, XIA Yifei5, ZHU Furong1, XIONG Wu1
1.The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China
2.Dongguan Hospital of Guangzhou University of TCM, Dongguan 523000, China
3.Hunan Provincial Encephalopathy Hospital, Changsha 410007, China
4.Clinical Medical School, Hunan University of Chinese Medicine, Changsha 410007, China
5.School of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China
关键词:
高糖环境; 肝细胞生长因子; 间充质干细胞; 生物学功能; 黄芪甲苷
Keywords:
high sugar environment; HGF; MSCs; biological function; AS-IV
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2023.11.06
文献标志码:
A
摘要:
目的探讨黄芪甲苷（astragaloside IV，AS-IV）对高糖受损间充质干细胞（mesenchymal stem cells，MSCs）生物学功能和分泌肝细胞生长因子（hepatocyte growth factor，HGF）的影响。 方法无菌条件下取足月健康新生儿胎盘脐带血，经密度梯度离心分离出人脐带血间充质干细胞（human umbilical cord blood mesenchymal stem cells，hUCBMSCs），分别进行成骨、成软骨和成脂诱导分化鉴定。将鉴定成功的hUCBMSCs用30 mmo1/L葡萄糖预处理120 h后，用不同浓度梯度AS-IV（0、50、100、200、300、400 mg/L）进行干预，确定AS-IV促进高糖受损hUCBMSCs增殖的最适浓度。另将高糖受损hUCBMSCs随机分为实验组和模型组，实验组用最适浓度AS-IV处理，模型组用等体积PBS液处理，同时设置正常组，即等量PBS液处理正常hUCBMSCs。通过CCK-8细胞增殖试验、黏附能力测定试验、细胞划痕试验及ELISA试验检测最适浓度AS-IV对hUCBMSCs增殖、黏附、迁移及分泌HGF功能的影响。 结果AS-IV改善受损hUCBMSCs增殖功能的最适浓度为300 mg/L；模型组高糖受损hUCBMSCs的增殖、黏附、迁移及分泌HGF功能低于正常组（P＜0.05）；实验组高糖受损hUCBMSCs的增殖、黏附、迁移及分泌HGF功能明显改善，与模型组比较差异具有统计学意义（P＜0.05）。 结论黄芪甲苷可促进高糖受损间充质干细胞增殖，改善其分泌功能。
Abstract:
ObjectiveTo investigate the effects of AS-IV on the biological functions and secretion of HGF in high glucose-damaged MSCs. MethodsHuman umbilical cord blood mesenchymal stem cells (hUCBMSCs) were isolated by density gradient centrifugation under aseptic conditions from placental cord blood of healthy full-term newborns and identified for osteogenic, chondrogenic and lipogenic induction of differentiation respectively. After successfully identified hUCBMSCs pretreated with 30 mmo1/L glucose for 120 h and then intervened with different gradients of AS-IV (0, 50, 100, 200, 300 and 400 mg/L), the optimal concentration of AS-IV was determined to promote proliferation of high glucose-impaired hUCBMSCs. In addition, high glucose-impaired hUCBMSCs were randomized into the experiment group and the model group, the experimental group was treated with the optimal concentration of AS-IV, and the model group was dealt with an equal volume of PBS solution, at the same time, the normal group was set, namely, processing normal hUCBMSCs with equivalent volume of PBS solution. The effect of the optimal concentration of AS-IV on the proliferation, adhesion, migration and HGF secretion of hUCBMSCs was measured by CCK-8 cell proliferation assay, adhesion assay, cell scoring assay and ELISA. ResultsThe optimal concentration of AS-IV which could improve the proliferation of damaged hUCBMSCs was 300 mg/L; the proliferation, adhesion, migration and HGF secretion functions of hUCBMSCs damaged by high glucose in the model group were lower than these of the normal group (P<0.05); the proliferation, adhesion, migration and HGF secretion functions of hUCBMSCs damaged by high glucose in the experiment group were significantly improved, and the difference was statistically significant (P<0.05). ConclusionAS-IV can protect high glucose-damaged MSCs and improve their secretory function.

相似文献/References:

[1]张晓凤,田耘,程小红,等.肾复康Ⅱ号对MSCs旁分泌作用的实验研究[J].西部中医药,2018,31(10):4.
　ZHANG Xiaofeng,TIAN Yun,CHENG Xiaohong,et al.Experimental Research into ShenFuKang Ⅱin Promoting Paracrine Function of MSCs[J].Western Journal of Traditional Chinese Medicine,2018,31(11):4.

备注/Memo

备注/Memo:
罗玉清（1988—），女，护师，研究方向：糖尿病性溃疡修复的基础研究。国家自然科学基金青年基金（81904217）；国家级大学生创新创业训练计划（S202010541015）；湖南中医药大学校级护理专项-重点项目（2019XJJ062）。
更新日期/Last Update: 2023-11-15