[1]徐静,王旭,房桂英,等.益气养阴方联合电针对产后压力性尿失禁大鼠CXCR4/COX-2信号通路的影响[J].西部中医药,2023,36(11):33-37.[doi:10.12174/j.issn.2096-9600.2023.11.07]
　XU Jing,WANG Xu,FANG Guiying,et al.The Effects of Benefiting-Qi Nourishing-Yin Prescription Combined with Electroacupuncture on CXCR4/COX-2 Signal Pathway in Rats with Postpartum Urinary Incontinence[J].Western Journal of Traditional Chinese Medicine,2023,36(11):33-37.[doi:10.12174/j.issn.2096-9600.2023.11.07]
点击复制

益气养阴方联合电针对产后压力性尿失禁大鼠CXCR4/COX-2信号通路的影响
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年11期
页码:
33-37
栏目:
出版日期:
2023-11-15

文章信息/Info

Title:
The Effects of Benefiting-Qi Nourishing-Yin Prescription Combined with Electroacupuncture on CXCR4/COX-2 Signal Pathway in Rats with Postpartum Urinary Incontinence
作者:
徐静, 王旭, 房桂英, 黄丽霞, 魏旭静
河北医科大学第一医院妇产科，河北 石家庄 050051
Author(s):
XU Jing, WANG Xu, FANG Guiying, HUANG Lixia, WEI Xujing
Department of Obstetrics and Gynecology, the First Hospital of Hebei Medical University, Shijiazhuang 050051, China
关键词:
产后压力性尿失禁; CXCR4/COX-2信号通路; 益气养阴方; 电针; 大鼠
Keywords:
postpartum urinary incontinence; CXCR4/COX-2 signal pathway; benefiting- nourishing- prescription; electroacupuncture; rats
分类号:
R246.3
DOI:
10.12174/j.issn.2096-9600.2023.11.07
文献标志码:
A
摘要:
目的探究益气养阴方联合电针对产后压力性尿失禁（postpartum urinary incontinence，PPUI）大鼠趋化因子受体4（chemokine receptor 4，CXCR4）/环氧合酶2（cyclooxygenase，COX-2）信号通路的作用机制。 方法将大鼠随机分为空白组、PPUI组、益气养阴组和益气养阴+电针组，制备PPUI大鼠模型；检测4组大鼠收缩力/肌重比值、血清乳酸脱氢酶（lactate dehydrogenase，LDH）和心肌酶肌酸激酶（creatine kinase，CK）含量；HE染色检测4组大鼠肛提肌组织病理学变化；蛋白质印迹法检测4组大鼠尿道组织CXCR4和COX-2蛋白表达。 结果与空白组相比，大鼠最大膀胱容量和漏尿压力点PPUI组降低（P＜0.05），益气养阴组和益气养阴+电针组升高，益气养阴+电针组升高明显（P＜0.05）；与空白组相比，收缩力/肌重比值PPUI组降低（P＜0.05），益气养阴组和益气养阴+电针组较PPUI组增加，且益气养阴+电针组增加更明显（P＜0.05）；与空白组相比，LDH和CK含量PPUI组升高（P＜0.05），益气养阴组和益气养阴+电针组低于PPUI组（P＜0.05）。与空白组相比，PPUI组CXCR4蛋白减少，COX-2蛋白增多（P＜0.05），益气养阴组和益气养阴+电针组CXCR4蛋白较PPUI组增多，COX-2蛋白较PPUI组减少（P＜0.05），益气养阴+电针组CXCR4蛋白较益气养阴组增加，COX-2蛋白较益气养阴组减少（P＜0.05）。 结论益气养阴方联合电针可能通过抑制PPUI大鼠血清LDH和CK表达，增加PPUI大鼠CXCR4表达，抑制COX-2表达，增加盆底肌肌肉收缩功能，治疗PPUI。
Abstract:
ObjectiveTo survey the mechanism of benefiting-Qi nourishing-Yin prescription in combination with electroacupuncture on CXCR4/COX-2 signal pathway in rats suffering postpartum urinary incontinence (PPUI). MethodsAll the rats were randomized into the blank group, PPUI group, benefiting-Qi nourishing-Yin group, benefiting-Qi nourishing-Yin+electroacupuncture group, to establish PPUI rat models; to detect contraction force/muscle weight ratio of the rats in four groups. The contents of LDH and CK; HE staining is applied to detect histopathological changes of musculi levator ani of the rats in four groups; Western blotting method to measure the expressions of CXCR4 and COX-2 protein in urethral tissue of the rats in four groups. ResultsCompared with the blank group, maximum bladder capacity and leakage pressure point of rats in PPUI group were decreased (P<0.05), after intervention, maximum bladder capacity and leakage pressure point of benefiting-Qi nourishing-Yin group and benefiting-Qi-Yin+electroacupuncture group were significantly restored, and the recovery in benefiting-Qi nourishing-Yin+electroacupuncture group was the best (P<0.05); compared with the blank group, the ratio of contractile force to muscle weight in the PPUI group was lowered (P<0.05), the ratio in benefiting-Qi nourishing-Yin group and benefiting-Qi nourishing-Yin+electroacupuncture group was significantly higher than that in the PPUI group, and the increase in benefiting-Qi nourishing-Yin+electroacupuncture group was more noticeable (P<0.05); compared with the blank group, the contents of LDH and CK increased in the PPUI group (P<0.05), benefiting-Qi nourishing-Yin group and benefiting-Qi nourishing-Yin+electroacupuncture group were lower than the PPUI group (P<0.05). Compared with the blank group, CXCR4 protein reduced while COX-2 protein increased in the PPUI group (P<0.05), the increase of CXCR4 protein in benefiting-Qi nourishing-Yin group and benefiting-Qi nourishing-Yin+electroacupuncture group were more than that in the PPUI group, COX-2 protein reduction was more obvious than that in the PPUI group (P<0.05), the increase of CXCR4 protein in benefiting-Qi nourishing-Yin+electroacupuncture group was higher than that in benefiting-Qi nourishing-Yin group, the contents of COX-2 protein were less than those in benefiting-Qi nourishing-Yin group (P<0.05). ConclusionBenefiting-Qi nourishing-Yin prescription in combination with electroacupuncture could increase the expressions of CXCR4 while restrain the expressions of COX-2 in PPUI rats, the contraction of pelvic floor muscle and treat PPUI possibly through inbibiting the expressions of LDH and CK in PPUI rats.

备注/Memo

备注/Memo:
徐静（1983—），女，硕士学位，副主任医师。研究方向：妇产科疾病的诊治。河北省医学科学研究课题计划项目（20200123）。
更新日期/Last Update: 2023-11-15