[1]王文英,李宝玲,黄宏羽,等.归龙丸对高糖诱导的RSC96神经膜细胞分泌TNF-α、IL-10的影响[J].西部中医药,2024,37(07):1-4.[doi:10.12174/j.issn.2096-9600.2024.07.01]
WANG Wenying,LI Baoling,HUANG Hongyu,et al.Effects of Guilong Pills on TNF-α and IL-10 Secretion of RSC96 Schwann Cells Induced by High Glucose[J].Western Journal of Traditional Chinese Medicine,2024,37(07):1-4.[doi:10.12174/j.issn.2096-9600.2024.07.01]
点击复制
归龙丸对高糖诱导的RSC96神经膜细胞分泌TNF-α、IL-10的影响
《西部中医药》[ISSN:2096-9600/CN:62-1204/R]
- 卷:
-
37
- 期数:
-
2024年07期
- 页码:
-
1-4
- 栏目:
-
基础研究
- 出版日期:
-
2024-07-15
文章信息/Info
- Title:
-
Effects of Guilong Pills on TNF-α and IL-10 Secretion of RSC96 Schwann Cells Induced by High Glucose
- 作者:
-
王文英, 李宝玲, 黄宏羽, 何万辉
-
广州医科大学附属中医医院,广东 广州 510130
- Author(s):
-
WANG Wenying, LI Baoling, HUANG Hongyu, HE Wanhui
-
The Affiliated TCM Hospital of Guangzhou Medical University, Guangzhou 510130, China
-
- 关键词:
-
归龙丸; 高糖; RSC96神经膜细胞; 肿瘤坏死因子; 血清白细胞介素10
- Keywords:
-
pills; high glucose; RSC96 Schwann cells; TNF-; IL-10
- 分类号:
-
R781.6+4
- DOI:
-
10.12174/j.issn.2096-9600.2024.07.01
- 文献标志码:
-
A
- 摘要:
-
目的评估归龙丸对高糖诱导的大鼠神经膜细胞(rats schwann cell,RSC96)分泌肿瘤坏死因子α(tumor necrosis factor α,TNF-α)、血清白细胞介素10(interleukin-10,IL-10)的影响。 方法将RSC96神经膜细胞复苏及培养,在含17 mmol/L的葡萄糖培养基中培养48 h。分为S1组(维生素D3+等渗乙醇溶液)、S2组(维生素D3+等渗乙醇溶液+归龙丸高剂量)、S3组(维生素D3+等渗乙醇溶液组+归龙丸中剂量)、S4组(维生素D3+等渗乙醇溶液组+归龙丸低剂量)、S5组(维生素D3+CYP24A1抑制剂genistein的乙醇溶液)等5组,S1~S4组中分别加入维生素D3及与其等浓度的乙醇,S5组加入维生素D3及CYP24A1抑制剂genistein的乙醇溶液,作用48 h。48 h后应用酶联免疫吸附试验法检测各组细胞上清液中TNF-α、IL-10的水平,并通过相应的基因引物检测RSC96细胞内CYP24A1m表达水平。 结果经归龙丸和CYP24A1抑制剂组处理后的神经膜细胞,其TNF-α的水平下降,IL-10水平上升,CYP24A1m表达水平下调。 结论归龙丸可能通过抑制CYP24A1蛋白表达,间接调节维生素D3使神经膜细胞TNF-α分泌减少、IL-10分泌增加,从而对高糖诱导的RSC96神经膜细胞起到保护作用。
- Abstract:
-
ObjectiveTo evaluate the effects of Guilong pills on the secretion of TNF-α and IL-10 in RSC96 Schwann cells induced by high glucose. MethodsAfter being resuscitated and cultured in a medium containing 17 mmol/L glucose for 48 h, RSC96 Schwann cells were divided into five groups: the S1 group (Vitamin D3+isotonic ethanol solution group), the S2 group (Vitamin D3+isotonic ethanol solution+high dose group of Guilong pills), the S3 group (Vitamin D3+isotonic ethanol solution+moderate dose group of Guilong pills), the S4 group (Vitamin D3+isotonic ethanol solution+low dose group of Guilong pills) and the S5 group (Vitamin D3+ethanol solution group of CYP24A1 inhibitor genistein), Vitamin D3 and the same concentration of ethanol solution were added respectively into the S1-S4 groups, ethanol solution of vitamin D3 and CYP24A1 inhibitor genistein was added to group S5 and acted for 48 hours. ELISA method was applied to detect the levels of TNF-α and IL-10 in the cell supernatant of each group in 48 hours, and the corresponding gene primer was used to measure the levels of CYP24A1m in RSC96 cells. ResultsThe levels of TNF-α decreased while the levels of IL-10 increased, the expressions of CYP24A1 downregulated in RSC96 Schwann cells processed with Guilong pills and CYP24A1m group. ConclusionGuilong pills could reduce the secretion of TNF-α and increase the levels of IL-10 possibly by inhibiting the expressions of CYP24A1 protein, and indirectly regulating the levels of Vitamin D3, thereby protecting RSC96 Schwann cells induced by high glucose.
备注/Memo
- 备注/Memo:
-
王文英(1975—),女,硕士学位,主任医师。研究方向:糖尿病及其慢性并发症的中医药诊治。E-mail:wwying75@126.com。广东省自然科学基金面上项目(2021A1515012547);第四批全国中医优秀人才研修项目(国中医药人教发〔2017〕24号);广州市中医药科技项目(20172A010012)。
更新日期/Last Update:
2024-07-15