[1]王文英,李宝玲,黄宏羽,等.归龙丸对高糖诱导的RSC96神经膜细胞分泌TNF-α、IL-10的影响[J].西部中医药,2024,37(07):1-4.[doi:10.12174/j.issn.2096-9600.2024.07.01]
　WANG Wenying,LI Baoling,HUANG Hongyu,et al.Effects of Guilong Pills on TNF-α and IL-10 Secretion of RSC96 Schwann Cells Induced by High Glucose[J].Western Journal of Traditional Chinese Medicine,2024,37(07):1-4.[doi:10.12174/j.issn.2096-9600.2024.07.01]
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归龙丸对高糖诱导的RSC96神经膜细胞分泌TNF-α、IL-10的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年07期
页码:
1-4
栏目:
基础研究
出版日期:
2024-07-15

文章信息/Info

Title:
Effects of Guilong Pills on TNF-α and IL-10 Secretion of RSC96 Schwann Cells Induced by High Glucose
作者:
王文英, 李宝玲, 黄宏羽, 何万辉
广州医科大学附属中医医院，广东 广州 510130
Author(s):
WANG Wenying, LI Baoling, HUANG Hongyu, HE Wanhui
The Affiliated TCM Hospital of Guangzhou Medical University, Guangzhou 510130, China
关键词:
归龙丸; 高糖; RSC96神经膜细胞; 肿瘤坏死因子; 血清白细胞介素10
Keywords:
pills; high glucose; RSC96 Schwann cells; TNF-; IL-10
分类号:
R781.6+4
DOI:
10.12174/j.issn.2096-9600.2024.07.01
文献标志码:
A
摘要:
目的评估归龙丸对高糖诱导的大鼠神经膜细胞（rats schwann cell，RSC96）分泌肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、血清白细胞介素10（interleukin-10，IL-10）的影响。 方法将RSC96神经膜细胞复苏及培养，在含17 mmol/L的葡萄糖培养基中培养48 h。分为S1组（维生素D3+等渗乙醇溶液）、S2组（维生素D3+等渗乙醇溶液+归龙丸高剂量）、S3组（维生素D3+等渗乙醇溶液组+归龙丸中剂量）、S4组（维生素D3+等渗乙醇溶液组+归龙丸低剂量）、S5组（维生素D3+CYP24A1抑制剂genistein的乙醇溶液）等5组，S1～S4组中分别加入维生素D3及与其等浓度的乙醇，S5组加入维生素D3及CYP24A1抑制剂genistein的乙醇溶液，作用48 h。48 h后应用酶联免疫吸附试验法检测各组细胞上清液中TNF-α、IL-10的水平，并通过相应的基因引物检测RSC96细胞内CYP24A1m表达水平。 结果经归龙丸和CYP24A1抑制剂组处理后的神经膜细胞，其TNF-α的水平下降，IL-10水平上升，CYP24A1m表达水平下调。 结论归龙丸可能通过抑制CYP24A1蛋白表达，间接调节维生素D3使神经膜细胞TNF-α分泌减少、IL-10分泌增加，从而对高糖诱导的RSC96神经膜细胞起到保护作用。
Abstract:
ObjectiveTo evaluate the effects of Guilong pills on the secretion of TNF-α and IL-10 in RSC96 Schwann cells induced by high glucose. MethodsAfter being resuscitated and cultured in a medium containing 17 mmol/L glucose for 48 h, RSC96 Schwann cells were divided into five groups: the S1 group (Vitamin D3+isotonic ethanol solution group), the S2 group (Vitamin D3+isotonic ethanol solution+high dose group of Guilong pills), the S3 group (Vitamin D3+isotonic ethanol solution+moderate dose group of Guilong pills), the S4 group (Vitamin D3+isotonic ethanol solution+low dose group of Guilong pills) and the S5 group (Vitamin D3+ethanol solution group of CYP24A1 inhibitor genistein), Vitamin D3 and the same concentration of ethanol solution were added respectively into the S1-S4 groups, ethanol solution of vitamin D3 and CYP24A1 inhibitor genistein was added to group S5 and acted for 48 hours. ELISA method was applied to detect the levels of TNF-α and IL-10 in the cell supernatant of each group in 48 hours, and the corresponding gene primer was used to measure the levels of CYP24A1m in RSC96 cells. ResultsThe levels of TNF-α decreased while the levels of IL-10 increased, the expressions of CYP24A1 downregulated in RSC96 Schwann cells processed with Guilong pills and CYP24A1m group. ConclusionGuilong pills could reduce the secretion of TNF-α and increase the levels of IL-10 possibly by inhibiting the expressions of CYP24A1 protein, and indirectly regulating the levels of Vitamin D3, thereby protecting RSC96 Schwann cells induced by high glucose.

相似文献/References:

[1]程涛,权卓,杨丽霞,等.新加糖肾康对高糖环境下HK-2细胞TGF-β/Smads信号通路的影响[J].西部中医药,2015,28(11):13.

备注/Memo

备注/Memo:
王文英（1975—），女，硕士学位，主任医师。研究方向：糖尿病及其慢性并发症的中医药诊治。E-mail：wwying75@126.com。广东省自然科学基金面上项目（2021A1515012547）；第四批全国中医优秀人才研修项目（国中医药人教发〔2017〕24号）；广州市中医药科技项目（20172A010012）。
更新日期/Last Update: 2024-07-15