[1]欧洪,郭静,王江瑞.玫芦消痤膏治疗湿疹作用机制的网络药理学研究[J].西部中医药,2024,37(07):43-49.[doi:10.12174/j.issn.2096-9600.2024.07.12]
　OU Hong,GUO Jing,WANG Jiangrui.Network Pharmacology Research on Meilu Xiaocuo Ointment in the Treatment of Eczema[J].Western Journal of Traditional Chinese Medicine,2024,37(07):43-49.[doi:10.12174/j.issn.2096-9600.2024.07.12]
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玫芦消痤膏治疗湿疹作用机制的网络药理学研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年07期
页码:
43-49
栏目:
二次研究
出版日期:
2024-07-15

文章信息/Info

Title:
Network Pharmacology Research on Meilu Xiaocuo Ointment in the Treatment of Eczema
作者:
欧洪1,2, 郭静2, 王江瑞3
1.成都中医药大学，四川 成都 610075
2.成都中医药大学附属医院，四川 成都 610075
3.贵州良济药业有限公司,贵州 龙里 551200
Author(s):
OU Hong1,2, GUO Jing2, WANG Jiangrui3
1.Chengdu University of TCM, Chengdu 610075, China
2.Hospital of Chengdu University of TCM, Chengdu 610075, China
3.Guizhou Liangji PharmaceuticalCo., LTD., Longli 551200, China
关键词:
湿疹; 玫芦消痤膏; 网络药理学; 靶点; 通路
Keywords:
eczema; ointment; network pharmacology; targets; pathway
分类号:
R758.23
DOI:
10.12174/j.issn.2096-9600.2024.07.12
文献标志码:
A
摘要:
目的研究苗药玫芦消痤膏治疗湿疹的作用机制，探索玫芦消痤膏治疗湿疹的作用靶标及信号途径。 方法在中药系统药理学数据库和分析平台，以及多个数据库中，联合检索筛选玫芦消痤膏活性成分-作用靶点，基因名注释使用UniProt数据库，并在OMIM数据库、GeneCards数据库、DrugBank数据库中检索湿疹的相关疾病靶标，再采用Rversion 3.6.2软件及Cytoscape 3.7.1软件构建玫芦消痤膏治疗湿疹的成分-作用靶点-通路网络，最后利用R语言中Bioc Manager包进行基因本体功能注释及KEGG通路富集分析。 结果经筛选得到玫芦消痤膏活性成分35个，槐定碱、槲皮素、苦参碱、木犀草素、鞣花酸、花生四烯酸、芦荟大黄素等是关键活性成分；作用靶标基因97个，核心蛋白有丝裂原活化蛋白激酶3前列腺素内过氧化物合酶，丝裂原活化蛋白激酶1、内皮一氧化氮合成酶、激素受体1、雄激素受体、脑源性神经营养因子、转录因子、过氧化物酶体增生激活受体等，参与调控高级糖基化终末产物-受体、肿瘤坏死因子、白细胞介素17、丝裂原活化蛋白激酶、PI3K-Akt等信号通路，从而发挥抗炎等作用。 结论玫芦消痤膏能有效缓解湿疹症状，可能与肿瘤坏死因子、PI3K-Akt等经典炎症通路有关，为后续分析玫芦消痤膏的药理机制和实验验证提供了理论支撑。
Abstract:
ObjectiveTo study the mechanism of Miao medicine Meilu Xiaocuo ointment in treating eczema, and to explore the targets of action and signaling pathways. MethodsThe active ingredients-target of action of Meilu Xiaocuo ointment were searched from the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and other databases, gene name annotation was performed using the UniProt database, eczema-related targets were retrieved from OMIM, GeneCards and DrugBank. The ingredients-targets of action-pathway network of Meilu Xiaocuo ointment in the treatment of eczema was constructed using Rversion 3.6.2 software and Cytoscape 3.7.1 software, consequently, BiocManager package in R language was utilized to perform gene ontology functional annotation and KEGG pathway enrichment analysis. ResultsA total of 35 active ingredients of the medicine were obtained, involving the key active ingredients such as sophoridine, quercetin, matrine, luteolin, tannin, arachidonic acid, aloe emodin; there were 97 target genes, and core proteins contained mitogen-activated protein kinase 3 (MAPK3), prostaglandin endoperoxidase synthase (PTGS), mitogen-activated protein kinase 1 (MAPK1), endothelial nitric oxide synthase (NOS), estrogen receptor 1(ESR1), androgen receptor (AR), brain-derived neurotrophic factor (BDNF), transcription factor and peroxisome proliferation-activated receptor (PPARG), participating in the regulation of signaling pathways such as AGE-RAGE, TNF, IL-17, MAPK and PI3K-Akt, thus playing anti-inflammatory role. ConclusionMeilu Xiaocuo ointment could effectively relieve the symptoms of eczema, and it might be related to classic inflammatory pathways including TNF and PI3K-Akt, which could provide theoretical support for the subsequent pharmacological mechanism analysis and experimental verification of Meilu Xiaocuo ointment.

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备注/Memo

备注/Memo:
欧洪（1989—），男，硕士学位，讲师。研究方向：中药资源与开发。国家自然科学基金（81873310）。
更新日期/Last Update: 2024-07-15