复方川草乌合剂镇痛抗炎作用的实验研究-《西部中医药》

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Title:: Experimental Study on the Analgesic and Anti-inflammatory Effects of Compound Chuancaowu Mixture

作者:: 肖登¹, 王建丁¹, 赵多明², 王晓怀², 张雪玲¹, 王俊玲¹, 李成云¹; 1.兰州大学公共卫生学院卫生毒理学系，甘肃兰州 730000
2.甘肃省中医院，甘肃兰州 730050

Author(s):: XIAO Deng¹, WANG Jianding¹, ZHAO Duoming², WANG Xiaohuai², ZHANG Xueling¹, WANG Junling¹, LI Chengyun¹; 1.Institute of Toxicology, School of Public Health, Lanzhou University, Lanzhou 730000, China
2.Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China

关键词:: 复方川草乌合剂; 肝肾毒性; 镇痛; 抗炎

Keywords:: compound mixture; hepatotoxicity; analgesic; anti-inflammatory

分类号:: R285.5

DOI:: 10.12174/j.issn.2096-9600.2024.09.04

文献标志码:: A

摘要:: 目的探究复方川草乌合剂对小鼠的肝肾毒性及其镇痛抗炎作用。方法将48只雄性ICR小鼠随机分为正常组、模型组及复方川草乌合剂低（0.33 g/mL）、中（0.66 g/mL）、高（1.32 g/mL）剂量组与正常+复方川草乌合剂高剂量组，每组8只。正常组小鼠不造模，其余各组用完全弗氏佐剂（complete freund′s adjuvant，CFA）于小鼠足底皮下注射染毒造模，复方川草乌合剂各剂量组灌胃相应剂量复方川草乌合剂，正常组和模型组灌胃双蒸水。检测不同时间点各组小鼠机械刺激痛阈值和热辐射痛阈值；干预7天后观察各组小鼠肝脏及肾脏组织病理学变化情况；酶联免疫吸附试验检测各组小鼠血清丙氨酸氨基转移酶（alanine aminotransferase，ALT）、谷胱甘肽氨基转移酶（glutathione aminotransferase，AST）、直接胆红素（direct bilirubin，DB）、总胆红素（total bilirubin，TB）、肌酐（creatinine，CRE）、尿酸（uric acid，UA）、尿素氮（UREA）含量以及足组织中白细胞介素1β（interleukin-1β，IL-1β）含量。结果与模型组比较，复方川草乌合剂以剂量依赖性提高CFA诱导小鼠的机械痛阈值和热辐射潜伏时间（P＜0.05）；正常组及正常+复方川草乌合剂高剂量组小鼠肝细胞呈放射状排列，未见明显异常，肝小叶结构清晰，肾小管整体结构完整；各组小鼠血清ALT、AST、DB、TB及CRE、UA、UREA含量比较差异均无统计学意义（P＞0.05）；与模型组比较，复方川草乌合剂中、高剂量组小鼠足组织IL-1β含量降低（P＜0.05）。结论复方川草乌合剂对CFA致慢性炎症性疼痛小鼠具有镇痛抗炎作用，且无耐受性，无明显肝肾毒性。

Abstract:: ObjectiveTo investigate the hepatotoxicity of compound Chuancaowu (Aconiti kusnezoffii Radix) mixture on mice, and the analgesic and anti-inflammatory effects of the medicine. MethodsAll 48 male ICR mice were randomized into the normal group, the model group, low (0.33 g/mL), moderate (0.66 g/mL) and high (1.32 g/mL) doses groups of compound Chuancaowu mixture, and normal+high doses group of compound Chuancaowu mixture with eight mice in each group. The mice remained unhandled, other groups were established into the models by subcutaneous injection of complete freund's adjuvant (CFA) into the plantar surface of mice, all the doses groups of compound Chuancaowu mixture were drenched with the corresponding doses of compound Chuancaowu mixture, the normal group and the model group accepted intragastric administration of double distilled water. To detect mechanical stimulation pain threshold and thermal radiation pain threshold of the mice in different groups at different time points; after seven days of intervention, to observe histopathological changes of liver and kidney in the mice of different groups; ELISA method was used to measure the contents of ALT, AST, DB, TB, CRE, UA and UREA of the mice in different groups and the levels of IL-1β in the foot. ResultsCompared with the model group, the mixture could lift mechanical stimulation pain threshold and latency time of thermal radiation in mice induced by CFA in the dose-dependent manner (P<0.05); the hepatocytes were arranged radially with no obvious abnormalities, the hepatic lobule structure was clear and the overall structure of the renal tubules was intact in the normal group and normal+high doses group of compound Chuancaowu mixture; the difference had no stastical meaning in the contents of ALT, AST, DB, TB, CRE, UA and UREA between different groups (P>0.05); compared with the model group, the contents of IL-1β were reduced in the feet of the mice in moderate and high dose groups of compound Chuancaowu mixture (P<0.05). ConclusionCompound Chuancaowu mixture has the analgesic and anti-inflammatory effects on CFA-induced chronic inflammatory pain in mice without tolerance and obvious hepatotoxicity.

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

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