[1]谢平金,柴生颋,陈群群,等.补肾健脾活血方对UMR106细胞成骨分化及ERα的影响[J].西部中医药,2024,37(11):1-5.[doi:10.12174/j.issn.2096-9600.2024.11.01]
　XIE Pingjin,CHAI Shengting,CHEN Qunqun,et al.Effect of Kidney-tonifying Spleen-invigorating Blood-activating Prescription on Osteogenic Differentiation and ERα of UMR106[J].Western Journal of Traditional Chinese Medicine,2024,37(11):1-5.[doi:10.12174/j.issn.2096-9600.2024.11.01]
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补肾健脾活血方对UMR106细胞成骨分化及ERα的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年11期
页码:
1-5
栏目:
基础研究
出版日期:
2024-11-15

文章信息/Info

Title:
Effect of Kidney-tonifying Spleen-invigorating Blood-activating Prescription on Osteogenic Differentiation and ERα of UMR106
作者:
谢平金1, 柴生颋2, 陈群群2, 卢启贵1, 张卫红1
1.上海中医药大学深圳医院/深圳市罗湖区中医院，广东 深圳 518000
2.广州中医药大学第三附属医院，广东 广州 510240
Author(s):
XIE Pingjin1, CHAI Shengting2, CHEN Qunqun2, LU Qigui1, ZHANG Weihong1
1.Shenzhen Hospital Affiliated to Shanghai University of TCM/Luohu District Hospital of Traditional Chinese Medicine, Shenzhen 518000, China
2.The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510240, China
关键词:
骨形成; 腺病毒; 分泌型卷曲相关蛋白1; 补肾健脾活血方
Keywords:
bone formation; adenovirus; SFRP1; kidney-tonifying spleen-invigorating blood-activating prescription
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2024.11.01
文献标志码:
A
摘要:
目的探讨补肾健脾活血方对过表达分泌型卷曲相关蛋白1（secreted frizzled related protein 1，SFRP1）、沉默SFRP1的UMR106细胞成骨分化及雌激素受体α（estrogen receptor α，ERα）的影响。 方法通过构建SFRP1过表达及沉默重组腺病毒载体，并转染大鼠类成骨细胞系UMR106细胞，初步分为空载腺病毒组、过表达SFRP1组、沉默SFRP1组，并根据含药血清和生理盐水（空白）血清干预的不同分为6组，观察6组细胞的碱性磷酸酶（alkaline phosphatase，ALP）活性及细胞ERα蛋白表达情况。 结果含药血清干预的空载腺病毒组、SFRP1沉默组及SFRP1过表达组72 h后UMR106细胞ALP活性和ERα蛋白表达均高于空白血清干预的空载腺病毒组、SFRP1沉默组及SFRP1过表达组（P＜0.05）；空白血清+SFRP1沉默组的UMR106细胞ALP活性及ERα蛋白表达高于空白血清+空载腺病毒组（P＜0.05），而空白血清+SFRP1过表达组的UMR106细胞ALP活性及ERα蛋白表达低于空白血清+空载腺病毒组（P＜0.05）。 结论过表达SFRP1可以抑制UMR106细胞成骨分化，并下调ERα蛋白表达；沉默SFRP1和补肾健脾活血方均可促进UMR106细胞成骨分化，并上调ERα蛋白表达，且两者共同干预时作用更显著，说明补肾健脾活血方能够抑制SFRP1表达，而SFRP1并不是补肾健脾活血方调节成骨细胞代谢，提高成骨分化活性和促进ERα蛋白表达的唯一靶点，可能存在其他靶点共同促进调节成骨细胞代谢。
Abstract:
ObjectiveTo explore the influence of kidney-tonifying spleen-invigorating blood-activating prescription on osteogenic differentiation and estrogen receptor α (ERα) of UMR106 cells via SFRP1 overexpression or gene knockdown. MethodsAfter constructing recombinant adenoviral vectors for SFRP1 overexpression or gene knockdown, rat osteoblast-like UMR106 cells, transfected with the virus, were primarily divided into empty adenovirus group, overexpression SFRP1 group and silencing SFRP1 group, and they were allocated to six groups according to the intervention with the medicated serum and physiological saline (blank) serum, to observe the activity of ALP and the expressions of cellular ERα in six groups. ResultsAfter intervening with medicated serum for 72 hours, the activity of ALP and the expressions of cellular ERα in empty adenovirus group, silencing SFRP1 group and overexpression SFRP1 group were higher than these of empty adenovirus group, silencing SFRP1 group and overexpression SFRP1 group intervened with blank serum (P<0.05); ALP activity and the expressions of ERα protein in blank serum+silencing SFRP1 group were higher than these of blank serum+empty adenovirus group (P<0.05), while ALP activity and the expressions of ERα protein in UMR106 cells of blank serum+overexpression SFRP1 group were lower than these in blank serum+empty adenovirus group (P<0.05). ConclusionOverexpression of SFRP1 could inhibit osteogenic differentiation of UMR106 cells and downregulate the expressions of ERα protein; Silencing of SFRP1 and kidney-tonifying spleen-invigorating blood-activating prescription could promote osteogenic differentiation of UMR106 cells, up regulate the expressions of ERα protein, and the effects are more evident when the two acted together, demonstrating that the formula could inhibit SFRP1 expression, but SFRP1 is not the only target in the process of the formula regulating the metabolism of osteoblast, improving the activity of osteogenic differentiation and ERα protein expressions, and the other targets may exist which could promote the regulation of osteoblast metabolism.

备注/Memo

备注/Memo:
谢平金（1992—），男，硕士学位，主治医师。研究方向：骨关节病、骨质疏松症的临床诊治及运动医学。广东省自然科学基金（2018A030310606）；广东省中医药管理局科研项目（20231295，20241258）；罗湖区软科学研究计划项目（LX202202133）。
更新日期/Last Update: 2024-11-15