基于Fisher判别分析的槐花、炒槐花标准汤剂特征图谱差异性研究-《西部中医药》

文章信息/Info

Title:: Study on the Differences of the Characteristic Mapping of Standard Decoction of Huaihua and Fried Huaihua Based on Fisher Discriminant Analysis

作者:: 高晗^1,2, 孙福仁^1,2, 李争¹, 李军山^1,2,3, 梁彩娟^1,2, 廖明丽^1,2, 李振江¹; 1.神威药业集团有限公司,河北石家庄 051430
2.石家庄市中药配方颗粒产业技术研究院,河北石家庄 050002
3.云南神威施普瑞药业有限公司,云南楚雄 675000

Author(s):: GAO Han^1,2, SUN Furen^1,2, LI Zheng¹, LI Junshan^1,2,3, LIANG Caijuan^1,2, LIAO Mingli^1,2, LI Zhenjiang¹; 1.China Shineway Pharmaceutical Group Ltd., Shijiazhuang 051430, China
2.Shijiazhuang Industrial Technology Research Institute of Traditional Chinese Medicine Formula Granules, Shijiazhuang 050002, China
3.Yunnan Shineway Spirin Pharmaceutical Co., Chuxiong 675000, China

Keywords:: fried; standard decoction; characteristic mapping; rutin; quercetin; Fisher discriminant analysis

摘要:: 目的建立槐花、炒槐花标准汤剂特征图谱，区分图谱差异。方法采用了UPLC色谱仪与WATERS BEH C18（2.1mm×100 mm，1.7 μm）色谱柱，以乙腈-0.1%磷酸水为流动相，柱温为35 ℃，流速为0.3 mL/min，检测波长为257 nm。结果应用特征图谱提取槐花、炒槐花标准汤剂特征图谱中特征成分，共确定5个特征峰。结论槐花、炒槐花标准汤剂UPLC特征图谱方法可靠、稳定，可用于槐花、炒槐花配方颗粒的定性鉴别。

Abstract:: ObjectiveTo establish the feature chromatograms of standard decoction of Huaihua (Sophora japonica L) and fried one, and to distinguish the differences in the mapping. MethodsA UPLC chromatographic instru-ment and WATERS BEH C18 (2.1 mm×100 mm, 1.7 μm) chromatographic column were employed, with acetonitrile-0.1% aqueous phosphate as the mobile phase, column temperature 35 ℃, flow rate 0.3 mL/min and the detection wavelength 257 nm. ResultsFive characteristic peaks were confirmed through the extraction of characteristic components from standard decoction of Huaihua and fried Huaihua with characteristic mapping. ConclusionThe method of UPLC characteristic spectrum of Huaihua and fried Huaihua, which is reliable and stable, could be used for qualitative identification of the formula granules.