[1]程胜强,何斌,彭志财.基于网络药理学探讨大补元煎促进脊柱压缩骨折愈合的机制[J].西部中医药,2025,38(03):75-83.[doi:10.12174/j.issn.2096-9600.2025.03.14]
　CHENG Shengqiang,HE Bin,PENG Zhicai.Network Pharmacology-based Exploration into the Mechanism of Promoting the Healing of Spinal Compression Fracture by Dabuyuan Decoction[J].Western Journal of Traditional Chinese Medicine,2025,38(03):75-83.[doi:10.12174/j.issn.2096-9600.2025.03.14]
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基于网络药理学探讨大补元煎促进脊柱压缩骨折愈合的机制()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年03期
页码:
75-83
栏目:
二次研究
出版日期:
2025-03-15

文章信息/Info

Title:
Network Pharmacology-based Exploration into the Mechanism of Promoting the Healing of Spinal Compression Fracture by Dabuyuan Decoction
作者:
程胜强1, 何斌2, 彭志财3
1.重庆市中医院，重庆 402160
2.中国中医科学院西苑医院，北京 100091
3.重庆医科大学附属永川中医院，重庆 402160
Author(s):
CHENG Shengqiang1, HE Bin2, PENG Zhicai3
1.Chongqing Hospital of Traditional Chinese Medicine, Chongqing 402160, China
2.Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
3.The Affiliated Yongchuan Hospital of Chongqing Medical University, Chongqing 402
关键词:
脊柱压缩性骨折; 大补元煎方; 网络药理学; 骨折愈合; 机制研究
Keywords:
spinal compression fracture; decoction; network pharmacology; fracture healing; mechanism study
分类号:
R274
DOI:
10.12174/j.issn.2096-9600.2025.03.14
文献标志码:
A
摘要:
目的通过网络药理学方法探讨大补元煎方促进脊柱压缩骨折愈合的机制。 方法在TCMSP数据库和SwissADME在线平台中筛选大补元煎方的化学成分，并通过Uniprot数据库转换成基因蛋白。在Gene-Cards、TTD和DisGeNET等数据库中筛选出“Compression fracture of spinal”的相关靶点。使用Venny 2.1得到药物和疾病共有基因，利用STRING和Cytoscape 3.8.2构建PPI（protein-protein interaction，PPI）网络图并通过网络拓扑分析获得关键蛋白靶点。使用Cytoscape 3.8.2的CytoHubb插件中的MCC（maximal clique centrality，MCC）算法筛选出核心基因和核心成分。使用Metascape在线平台进行基因本体论富集分析GO（gene ontology，GO）及京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路功能富集分析。使用R软件对GO及KEGG富集结果绘制条形图及气泡图。使用Cytoscape 3.8.2构建大补元煎方治疗脊柱压缩骨折的通路-基因网络图。 结果大补元煎方中的中药有效活性成分共124个，潜在作用靶点271个，脊柱压缩性骨折相关作用靶点875个，交集基因剔除游离节点77个，核心基因有10个，核心活性成分有7个。GO分析结果显示：炎症反应、细菌反应、血管发育、细胞囊、细胞外基质、膜筏、受体调节活性、转录因子结合、细胞因子受体结合等排名靠前；KEGG分析结果显示，骨折愈合关键基因有：丝裂原活化蛋白激酶1（mitogen-activated protein kinase1，MAPK1）、丝裂原活化蛋白激酶3（mitogen-activated protein kinase1，MAPK3）转化生长因子β1（transforming growth factor-β，TGF-β1）、白细胞介素6（interleukin-6，IL-6）等；关键通路有：MAPK、磷脂酰肌醇3激酶-蛋白激酶B（PI3K-AKT signal pathway，PI3K-AKT）、低氧诱导因子1（hypoxia inducible factor-1，HIF-1）等信号通路。 结论大补元煎方的核心活性成分通过调控细胞因子、生长因子和转录因子，如MAPK1、MAPK3、TGF-β1、IL-6等因子激活MAPK、PI3K-AKT、HIF-1等信号通路，调节细胞的生成与凋亡，促进新血管生成、成骨细胞的分化，抑制炎症反应、破骨细胞的生成，调整破骨细胞与成骨细胞之间代谢稳态平衡来促进骨折愈合。
Abstract:
ObjectiveTo explore the mechanism of promoting the healing of spinal compression fracture by Dabuyuan decoction using network pharmacology. MethodsThe chemical ingredients of Dabuyuan decoction were screened in TCMSP database and SwissADME online platform, and transformed into gene proteins through UniProt database. The relevant targets of "compression fracture spinal of" were screened via GeneCards, TTD and DisGeNET. Venny 2.1 was applied to obtain the shared genes of medicine and disease, STRING and Cytoscape 3.8.2 were utilized to construct PPI network, and network topology was applied to analyze and obtain key protein targets; MCC (maximal clique centrality) algorithm in the CytoHubb plug-in of Cytoscape 3.8.2 was used to screen the core genes and core ingredients. Metascape online platform was utilized to perform functional enrichment analysis of GO and KEGG pathway; R software was applied to draw bar chart and bubble diagram of GO and KEGG enrichment results. Cytoscape 3.8.2 was utilized to construct pathway-gene network diagram of the decoction in the treatment of spinal compression fracture. ResultsThere were 124 effective active ingredients of the herbs contained in the decoction, 271 potential targets of action, 875 targets related to spinal compression fracture, 77 free nodes removed by intersection genes, ten core genes and seven core active ingredients. GO analysis results revealed that these factors ranked high, including inflammatory reaction, bacterial reaction, vascular development, cell capsules, extracellular matrix, membrane raft, receptor regulatory activity, transcription factor binding and cytokine receptor binding; KEGG analysis results showed that the key genes of fracture healing were MAPK1, MAPK3, TGF-β1 and IL-6; key pathways contained MAPK, PI3K-Akt and HIF-1. ConclusionThe core active ingredients of the decoction could regulate cell production and apoptosis, and promote the neoan-giogenesis and osteoblast differentiation, inhibit inflammatory response and the formation of osteoclasts, as well as adjust the metabolic homeostasis balance between osteoclasts and osteoblasts through regulating cytokines, growth factors and transcription factors including MAPK1, MAPK3, TGF-β1 and IL-6 to activate signaling pathways like MAPK, PI3K-AKT and HIF-1, so as to promote fracture healing.

备注/Memo

备注/Memo:
程胜强（1990—），男，硕士学位，主治中医师。研究方向：脊柱外科疾病及神经病理性疼痛的临床诊治。国家自然科学基金（81973676）；重庆市永川区科学技术局自然科学基金（2021yc-jckx20016）。
更新日期/Last Update: 2025-03-15