[1]贺敏,蒋健,朱盼盼,等.从内源性抗菌肽研究尿感方对膀胱上皮细胞感染早期防御响应的影响[J].西部中医药,2026,39(01):12-16.[doi:10.12174/j.issn.2096-9600.2026.01.03]
　HE Min,JIANG Jian,ZHU Panpan,et al.Investigating the Effects of "Urinary-infection Prescription" on Early Defensive Response in Bladder Epithelial Cell Infection via Antimicrobial Peptides[J].Western Journal of Traditional Chinese Medicine,2026,39(01):12-16.[doi:10.12174/j.issn.2096-9600.2026.01.03]
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从内源性抗菌肽研究尿感方对膀胱上皮细胞感染早期防御响应的影响()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
39
期数:
2026年01期
页码:
12-16
栏目:
基础研究
出版日期:
2026-01-15

文章信息/Info

Title:
Investigating the Effects of "Urinary-infection Prescription" on Early Defensive Response in Bladder Epithelial Cell Infection via Antimicrobial Peptides
作者:
贺敏, 蒋健, 朱盼盼, 乔昀, 陈君灏, 张雯霞
上海中医药大学附属曙光医院，上海 201203
Author(s):
HE Min, JIANG Jian, ZHU Panpan, QIAO Yun, CHEN Junhao, ZHANG Wenxia
Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China
关键词:
尿路感染; 膀胱上皮细胞; 内源性抗菌肽; 尿道致病性大肠杆菌; 白细胞介素6; 转录激活因子3; 尿感方
Keywords:
urinary tract infection; bladder epithelial cells; antimicrobial peptides; uropathogenic escherichia coli; IL-6; activating transcription factor 3; urinary-infection prescription
分类号:
R256.52
DOI:
10.12174/j.issn.2096-9600.2026.01.03
文献标志码:
A
摘要:
目的从内源性抗菌肽（antimicrobial Peptides，AMPs）的表达观察尿感方对膀胱上皮细胞（bladder epithelial cells，BECs）感染早期防御响应的影响，并从IL-6/Stat3信号通路探讨其作用机理。 方法采用RT-PCR法检测尿道致病性大肠杆菌感染早期BECs内源性抗菌肽CAMP、DEFB1、脂质运载蛋白2（Lipocalin 2，LCN2）、核糖核酸酶4（Ribonuclease 4，RNases4）和RNase7 mRNA的表达，并观察细菌定植率，同时采用ELISA法检测白细胞介素6（Interleukin-6，IL-6）水平，蛋白免疫印迹法检测p-Stat3蛋白表达。 结果与模型组比较，5%、10%大鼠空白尿液组AMPs（CAMP、DEFB1、LCN2、RNases4和Nases7）的mRNA表达、细菌定植率、IL-6水平和p-Stat3表达均无明显差异（P＞0.05）；与5%大鼠空白尿液组比较，5%大鼠含药尿液组Nases7的mRNA表达上调（P＜0.05）；与10%大鼠空白尿液组比较，10%大鼠含药尿液组AMPs（CAMP、DEFB1、LCN2、RNases4和Nases7）的mRNA表达、IL-6水平和p-Stat3表达均上调（P＜0.05），细菌定植率下降（P＜0.05）。 结论尿感方能增加感染早期BECs的AMPs表达，强化BECs对病原菌的快速防御响应，减少细菌定植，其部分作用机制与IL-6/Stat3信号传导通路有关。
Abstract:
ObjectiveTo observe the effects of urinary-infection prescription on early defensive response in bladder epithelial cells (BECs) infection via the expressions of AMPs, and to survey its mechanism from IL-6/Stat3 signaling pathway. MethodsRT-PCR was applied to detect the expressions of AMPs including CAMP, DEFB1, LCN2, RNases4 and RNase7 mRNA in BECs at early stage of uropathogenic escherichia coli (UPEC) infection, to observe bacterial colonization rate, meanwhile ELISA was used to measure the levels of IL-6, Western blotting to monitor the expressions of p-Stat3 protein. ResultsNo significant difference existed in the expressions of mRNA of AMPs including CAMP, DEFB1, LCN2, RNases4 and Nases7, bacterial colonization rate, the levels of IL-6 and p-Stat3 when 5% and 10% normal rat urine control groups were compared with the model group (P>0.05); the upregulation of Nases7 mRNA was observed when 5% drug-containing rat urine group was compared with 5% normal rat urine control group (P<0.05); 10% drug-containing rat urine group demonstrated the increase in mRNA expressions of AMPs including CAMP, DEFB1, LCN2, RNases4 and Nases7, the levels of IL-6 and p-Stat3, while the decrease in bacterial colonization rate when compared with 10% normal rat urine control group (P<0.05). ConclusionUrinary-infection prescription could enhance the expressions of AMPs in BECs during the early stage of infection, enhance rapid defense response of BECs against pathogenic bacteria, and reduce bacterial colonization rate, and part of the mechanism is related to IL-6/Stat3 signaling pathway.

相似文献/References:

[1]王建平,赵敏,成敏洁,等.循证护理预防长期留置导尿老年男性患者尿路感染的价值[J].西部中医药,2014,27(11):148.
[2]姚秋霞.效应分析管理模式联合扶正愈淋方降低导尿管相关尿路感染风险的临床观察[J].西部中医药,2019,32(04):134.
　YAO Qiuxia.Clinical Observation on Failure Mode and Effects Analysis Joined with FuZheng YuLin Prescription in Decreasing the Risk of Catheter-associated Urinary Tract Infection[J].Western Journal of Traditional Chinese Medicine,2019,32(01):134.

备注/Memo

备注/Memo:
国家自然科学基金（81874362，82104635）。贺敏（1977—），女，博士学位，研究员。研究方向：中医药防治感染性疾病的临床基础研究。Email：heminmiao@163.com。
更新日期/Last Update: 2026-01-15