[1]黄舒然,王瑞平△,邹 玺,等.蜂毒素对人胃癌细胞株BGC-823凋亡及其细胞周期的影响*[J].西部中医药,2012,25(10):15-17.
 HUANG Shu-ran,WANG Rui-ping,ZOU Xi,et al. Effects of Melittin on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line BGC-823[J].Western Journal of Traditional Chinese Medicine,2012,25(10):15-17.
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蜂毒素对人胃癌细胞株BGC-823凋亡及其细胞周期的影响*()
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
25
期数:
2012年10期
页码:
15-17
栏目:
出版日期:
2012-10-15

文章信息/Info

Title:
 Effects of Melittin on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line BGC-823
文章编号:
1004-6852(2012)10-0015-03
作者:
 黄舒然1王瑞平12△邹 玺12周锦勇12
 1 南京中医药大学第一临床医学院,江苏 南京 210046; 2 江苏省中医院
Author(s):
 HUANG Shu-ran1 WANG Rui-ping12 ZOU Xi12 ZHOU Jin-yong12
 1 Nanjing University of Chinese Medicine, Nanjing 210046, China;
2 Jiangsu Province Hospital of Traditional Chinese Medicine
关键词:
 蜂毒素人胃癌细胞株BGC-823细胞增殖细胞凋亡细胞周期
Keywords:
 melittin human gastric cancer cell line BGC-823 cell proliferation apoptosis cell cycle
分类号:
R730.5
文献标志码:
A
摘要:
 目的:观察蜂毒素体外对人胃癌细胞株BGC-823增殖、凋亡及细胞周期的影响。方法:采用MTT法观察不同浓度的蜂毒素(32,16,8,4,2 μg/mL)体外对人胃癌细胞株BGC-823增殖的影响,并用流式细胞仪检测其诱导的细胞凋亡和周期阻滞效应。结果:①与对照组比较,蜂毒素有抑制人胃癌细胞株BGC-823增殖的作用(P <0.01),随着药物浓度的增大,其对细胞的抑制率也明显增大,呈现剂量依赖性。②蜂毒素浓度从
4 μg/mL开始,即可诱导细胞产生凋亡,随着剂量的增加诱导凋亡的效果更加明显。③在细胞周期方面,与阴性对照比较,蜂毒素作用24小时后S期细胞比例上升,而G0/G1期比例明显下降,蜂毒素浓度达到16 μg/mL时G0/G1期前出现明显的亚二倍体区。结论:蜂毒素对人胃癌细胞株BGC-823的增殖具有抑制作用,且能够引起该细胞的凋亡及改变其细胞周期。
Abstract:
 Objective: To investigate effect of melittin in vitro on proliferation, apoptosis and cell cycle of human gastric cancer cell line BGC-823. Method: Effects of different concentrations of melittin (32, 16, 8, 4 or 2 μg/mL) on proliferation of human gastric cancer cell line BGC-823 were investigated with MTT assay; induced apoptosis and cycle blocking were detected with flow cytometer (FCM). Result: ① Compared with control group, melittin inhibited proliferation of human gastric cancer cell line BGC-823(P<0.01). The inhibition effects increased with the dose. ② Melittin induced cell apoptosis when the concentration reached to 4 μg/mL, the apoptosis-inducing effect rose with the dose of melittin. ③ As cell cycle, the percentage of cells in S phase increased after melittin working for 24 hours while the ratio of the cells in G0/G1 phase decreased compared with negative control group, when the concentration of melittin reached to 16 μg/mL, the sub-diploid peak showed obviously before G0/G1 phase. Conclusion: Melittin could inhibit the proliferation of human gastric cancer cell line BGC-823, induce the apoptosis of cells and change its cell cycle.

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期:2012-04-12
*基金项目:江苏省六大高峰人才项目,江苏省中医药管理局领军人才项目(编号LJ200908),江苏省高校优势学科资助项目。
作者简介:黄舒然(1986—),女,硕士研究生。研究方向:肿瘤疾病的中西医结合诊治。
△通讯作者:王瑞平(1961—),女,博士研究生导师,博士学位,教授,主任医师。研究方向:肿瘤疾病的中西医结合诊治。
更新日期/Last Update: 2012-10-29