[1]薛建军,张凌云,贾国龙,等.参附注射液对心肌缺血再灌注老年大鼠NF—κB p65及COX—2表达的影响[J].西部中医药,2012,25(08):14-16.
　XUE Jian-jun,ZHANG Ling-yun,JIA Guo-long,et al.Influence of ShenFu Injection on Expressions of NF-κB p65 and COX-2 in Aged Myocardial Ischemia Reperfusion Rat Model[J].Western Journal of Traditional Chinese Medicine,2012,25(08):14-16.
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参附注射液对心肌缺血再灌注老年大鼠NF—κB p65及COX—2表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年08期
页码:
14-16
栏目:
出版日期:
2012-08-15

文章信息/Info

Title:
Influence of ShenFu Injection on Expressions of NF-κB p65 and COX-2 in Aged Myocardial Ischemia Reperfusion Rat Model
文章编号:
1004-6852(2012)08-0014-03
作者:
薛建军1，张凌云1，贾国龙1，呼荟茹2，齐兵献2，贾育新2△
1 甘肃省中医院麻醉科，甘肃 兰州 730050； 2 甘肃中医学院
Author(s):
XUE Jian-jun1, ZHANG Ling-yun1, JIA Guo-long1, HU Hui-ru2, QI Bin-xian2, JIA Yu-xin2△
1 Department of Anesthesiology in Gansu Province Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu University of Traditional Chinese Medicine
关键词:
心肌缺血再灌注; 参附注射液; 心肌酶; NF-κB p65; COX-2
Keywords:
myocardial ischemia reperfusion;  ShenFu injection;  cardiac enzymes;  NF-κB p65;  COX-2
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨参附注射液对心肌缺血再灌注老年大鼠心肌组织NF-κB p65及COX-2的影响。方法：健康Wistar老年大鼠30只，18月龄，随机分为3组，假手术组(S组)、缺血再灌注组(I/R组)、参附注射液组(SF组)，每组10只。采用结扎左冠状动脉前降支制备心肌缺血再灌注模型。观察参附注射液对缺血再灌注老年大鼠血清中肌酸激酶同工酶(CK-KB)、天门冬氨基转移酶(AST)、乳酸脱氢酶(LDH)溢出量的影响；运用免疫组化法观察心肌缺血再灌注后心肌组织细胞中NF-κB p65的活性及COX-2的蛋白表达。结果：参附注射液能显著减少I/R老年大鼠血清中CK-KB、AST、LDH的溢出量。与S组比较，I/R组心肌组织NF-κB p65的活性增高，COX-2的蛋白表达明显增加(P＜0.05)。SF组中NF-κB p65、COX-2显著下降(P＜0.05)。结论：NF-κB p65和COX-2在心肌缺血再灌注中均有高水平表达，提示炎症参与了心肌缺血再灌注，机制可能是NF-κB p65通过对COX-2的转录调控发挥了重要作用;参附注射液可抑制NF-κB p65活性，降低COX-2的蛋白表达水平，减轻I/R心肌炎症反应，对心肌起保护作用。
Abstract:
Objective: To study the effects of ShenFu injection on expressions of NF-κB p65 and COX-2 in aged rat model with myocardial ischemia reperfusion. Method: Thirty aged wistar rats were randomly divided into three groups (n=10 each): sham group(S group); ischemia reperfusion group (I/R group) and ShenFu group (SF group). Model with myocardial ischemia reperfusion was established through left anterior descending coronary artery ligation. Influence of ShenFu injection on spillage of creatine phosphokinase isoenzyme(CK-KB), aspartate amino transferase (AST) and lactate dehydrogenase(LDH) in serum of aged rat with ischemia reperfusion were observed; activity of NF-κB p65 and expression of COX-2 in myocardial tissue after reperfusion were observed with immunohistochemical method. Result: Spillage of CK-KB, AST and LDH in serum of aged rats in I/R group decreased significantly. Compared with S group, activity of NF-κB p65 and protein expression of COX-2 improved in myocardial tissue of I/R group(P＜0.05). Above indexes in SF group dropped down remarkably. Conclusion: High level expression of NF-κB p65 and COX-2 demonstrated that inflammation participate in myocardial ischemia reperfusion. The mechanism might be NF-κB p65 play an important role through transferring and regulating COX-2. ShenFu injection can inhibit the activity of NF-κB p65, reduce expression level of COX-2, alleviate inflammatory reaction and protect myocardial muscle.

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备注/Memo

备注/Memo:
收稿日期：2012-02-27 *基金项目：甘肃省自然科学研究基金项目(编号1010RJZA209) 作者简介：薛建军(1971—)，男，硕士学位，副主任医师。研究方向：麻醉与循环。 △通讯作者：贾育新(1962—)，女，教授，副主任医师。研究方向：中医证候理论研究和临床应用。
更新日期/Last Update: 2012-08-15