[1]许珊丽,王恒瑞,于双,等.归芪多糖对免疫低下小鼠迟发型超敏反应的影响*[J].西部中医药,2013,26(05):7-9.
 XU Shanli,WANG Hengrui,YU Shuang,et al.Effects of Angelica and Astragalus Polysaccharide on Delayed Type Hypersensitivity of Immunosuppressive Mice[J].Western Journal of Traditional Chinese Medicine,2013,26(05):7-9.
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归芪多糖对免疫低下小鼠迟发型超敏反应的影响*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年05期
页码:
7-9
栏目:
出版日期:
2013-05-15

文章信息/Info

Title:
Effects of Angelica and Astragalus Polysaccharide on Delayed Type Hypersensitivity of Immunosuppressive Mice
文章编号:
1004-6852(2013)05-0007-03
作者:
许珊丽1王恒瑞2于双2樊文博2蒲秀瑛2△
1 兰州市第一人民医院,甘肃 兰州 730050;2 兰州理工大学
Author(s):
XU Shanli1, WANG Hengrui2, YU Shuang2, FAN Wenbo2, PU Xiuying2△
1 The First People's Hospital, Lanzhou 730050, China; 2 Lanzhou University of Technology
关键词:
超敏反应迟发型环磷酰胺耳肿胀度免疫脏器指数归芪多糖
Keywords:
hypersensitivity delayed type cyclophosphamide (CY) the swollen degree of auriclethe indexes of immune organs angelica and astragalus polysaccharide
分类号:
R392.12
文献标志码:
A
摘要:
目的:探讨归芪多糖对免疫低下小鼠迟发型超敏反应(DTH)的影响。方法:将 40 只昆明种小鼠随机分为正常对照组、模型对照组、归芪多糖高剂量组(100 mg/kg)、归芪多糖低剂量组(50 mg/kg)和阳性药物对照组[黄芪精口服液(1.8 g/kg)],每组 8 只。除正常对照组外其余各组通过皮下注射环磷酰胺(CY)建立免疫抑制小鼠模型。模型对照组灌服等体积生理盐水,各药物组分别灌服归芪多糖(50 mg/kg 和 100 mg/kg)和黄 芪精口服液(1.8 g/kg),1 次 /d,连续灌服 6 天后,除正常对照组外其余各组以二硝基氟苯(DNFB)致敏并加强2 天后计算小鼠耳廓肿胀度及脾脏、胸腺指数。结果:100 mg/kg 归芪多糖能明显增加免疫抑制小鼠耳廓肿胀度,与阳性对照药物黄芪精口服液相比无显著性差异;且归芪多糖在 50-100mg/kg 剂量范围内可对抗环磷酰胺所致小鼠脾脏和胸腺的萎缩,增加小鼠免疫脏器指数。结论:归芪多糖可恢复小鼠对外源性过敏原所导致的迟发性超敏反应,且能对抗环磷酰胺引起的脾脏和胸腺的萎缩。
Abstract:
Objective: To explore the effects of angelica and astragalus polysaccharide on delayed type hypersensitivity (DTH) of immunosuppressive mice. Method: All 40 mice were randomized into normal control group, model control group, high (100 mg/kg) and low (50 mg/kg) dosage groups of angelica and astragalus polysaccharide, and the control group of positive drug (HuangQiJing oral liquid, 1.8 g/kg), eight mice each group.Except normal control group, immunosuppressive mice models were established with hypodermic injection of cyclophosphamide (CY). Normal control group received the same amount of physiological saline and other groups were given with angelica and astragalus polysaccharide (100 mg/kg and 50 mg/kg) and HuangQiJing oral liquid (1.8 g/kg), once each day, for six consecutive days, except normal control group, other groups were sensitized with dinitrofluorobenzene (DNFB) and enhanced for two days to calculate the swollen degree of auricle, the indexes of spleen and thymus. Result: Compared with HuangQiJing oral liquid, 100mg/kg angelica and astragalus polysaccha-ride enhanced the swollen degree of auricle and showed no significant difference; angelica and astragalus polysaccharide could resist the atrophy of spleen and thymus in mice induced by CY and enhance the index of immune organs in mice. Conclusion: Angelica and astragalus polysaccharide could recover delayed type hypersensi-tivity and resist the atrophy of the spleen and thymus induced by CY.

备注/Memo

备注/Memo:
收稿日期:2012-12-11 * 基金项目:甘肃省自然科学基金项目(编号 1112RJZA021),兰州市科技局项目(编号 2011-1-71) 作者简介:许珊丽(1967—),女,副主任医师。研究方向:心血管内科疾病的诊治。 △通讯作者:蒲秀瑛(1968—),女,博士学位,硕士生导师,副教授。研究方向:药理学与毒理学。
更新日期/Last Update: 2013-05-15