[1]赵庆大,刘萍,蔡乐,等.两益汤对哮喘豚鼠引喘潜伏期及TNF-α、IL-4 水平的影响[J].西部中医药,2013,26(05):16-18.
　ZHAO Qingda,LIU Ping,CAI Le,et al.Effects of LiangY iTang on Incubation Period of Asthma and Levels of TNF-α and IL-4 in Asthmatic Guinea Pigs[J].Western Journal of Traditional Chinese Medicine,2013,26(05):16-18.
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两益汤对哮喘豚鼠引喘潜伏期及TNF-α、IL-4 水平的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年05期
页码:
16-18
栏目:
出版日期:
2013-05-15

文章信息/Info

Title:
Effects of LiangY iTang on Incubation Period of Asthma and Levels of TNF-α and IL-4 in Asthmatic Guinea Pigs
文章编号:
1004-6852(2013)05-0016-03
作者:
赵庆大，刘萍，蔡乐，李游
中国人民解放军总医院，北京 100853
Author(s):
ZHAO Qingda, LIU Ping, CAI Le, LI You
Chinese PLA General Hospital, Beijing 100853, China
关键词:
哮喘; 两益汤; 引喘潜伏期; TNF-α; IL-4
Keywords:
asthma;  LiangYiTang;  incubation period of asthma;  TNF-α;  IL-4
分类号:
R562.25
文献标志码:
A
摘要:
目的：观察两益汤对哮喘豚鼠引喘潜伏期和血清 TNF-α 和 IL-4 水平的影响，探讨其防治哮喘的机理。方法：采用 2%氯化乙酰胆碱和 0.1%磷酸组胺等体积混合液制备急性豚鼠哮喘模型，观察生理盐水（A组）、两益汤（B 组）和氨茶碱（C 组）对哮喘潜伏期的影响；以卵蛋白致敏法制备慢性豚鼠哮喘模型，应用酶联免疫吸附试验法测定生理盐水组（D 组）、两益汤组（E 组）和地塞米松组（F 组）豚鼠血清 TNF-α 和 IL-4 的表达水平。结果：与生理盐水组相比，两益汤组能明显延长豚鼠哮喘潜伏期（P＜0.01)，两益汤组与氨茶碱组相比无明显差异（P＞0.05)；两益汤组能显著降低哮喘状态下豚鼠血清 TNF-α（P＜0.05)，并能显著降低哮喘状态下豚鼠血清 IL-4 的水平（P＜0.01)。结论：两益汤有延长哮喘豚鼠引喘潜伏期的作用，其对哮喘呼吸道炎症的抑制可能与下调哮喘豚鼠 TNF-α 和 IL-4 的表达有关。
Abstract:
Objective: To observe the effects of LiangYiTang on the latency period of asthma and the levels of TNF-α and interleukine-4 (IL-4) in guinea pig, and explore the mechanism of preventing asthma. Method: Acute asthmatic models were established with the mixture of 2% acetylcholine chloride and 0.1% histamine phosphate to observe the influence of physiological saline(Group A), LiangYiTang(Group B) and aminophylline (Group C) on the incubation period of asthma; chronic asthmatic models were built with ovalbumin challenge method. Expressions of TNF-α and IL-4 in the groups of physiological saline(D), LiangYiTang(E) and dexamethasone(F) were detected with enzyme-linked immunosorbent assay (ELISA). Result: Compared with the group of physiological saline,LiangY iTang could prolong the latency period of asthma in guinea pig significantly (P＜ 0.01), comparisons between LiangY iTang group and aminophylline group had no significant difference(P＞ 0.05); LiangY iTang could lower serum TNF-α of asthmatic guinea pig significantly (P＜0.05) and level of IL-4 (P＜0.01). Conclusion: LiangYiTang could delay the latency period of asthma in asthmatic guinea pig, the function of inhibition to the inflammation of respiratory tract might be related to down regulation the expression of TNF-α and IL-4.

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备注/Memo

备注/Memo:
收稿日期：2012-11-06 作者简介：赵庆大(1982—)，男，医师。研究方向：肿瘤疾病的中医诊治。
更新日期/Last Update: 2013-05-15