[1]郭钰龙,王谨慧,靳子明,等.RP-HPLC法测定展筋酊中大黄素、大黄酚的含量*[J].西部中医药,2013,26(05):24-27.
 GUO Yulong,WANG Jinhui,JIN Ziming,et al.Content Determination of Emodin and Chrysophanol in ZhanJing Tincture by RP-HPLC[J].Western Journal of Traditional Chinese Medicine,2013,26(05):24-27.
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RP-HPLC法测定展筋酊中大黄素、大黄酚的含量*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年05期
页码:
24-27
栏目:
方药·质量分析
出版日期:
2013-05-15

文章信息/Info

Title:
Content Determination of Emodin and Chrysophanol in ZhanJing Tincture by RP-HPLC
文章编号:
1004-6852(2013)05-0024-04
作者:
郭钰龙1王谨慧2靳子明2陶翠祥2杨锡仓2△
1 甘肃中医学院,甘肃 兰州 730020;2 甘肃中医学院附属医院
Author(s):
GUO Yulong1 WANG Jinhui2 JIN Ziming2 TAO Cuixiang2 YANG Xicang2△
University Hospital of Gansu Traditional Chinese Medicine, Lanzhou
关键词:
展筋酊HPLC 法大黄素大黄酚含量测定
Keywords:
ZhanJing tincture HPLC method emodin chrysophanol content determination
分类号:
R284
文献标志码:
A
摘要:
目的:建立展筋酊中大黄素,大黄酚的 RP-HPLC 测定方法。方法:Symmetry C18 色谱柱(5.0μm),甲醇∶0.1%磷酸溶液 (85∶15) 为流动相,流速:0.8 mL/min;柱温:20℃,检测波长:423 nm。结果:大黄素、大黄酚在 10.0-30.0μg/mL浓度范围内与峰面积呈良好的线形关系(r=0.999 9,0.999 7);平均回收率分别为101.35%和98.25%(RSD=2.26%,1.17%)。结论:本方法快速、灵敏、专属性强,可用于展筋酊的含量测定。
Abstract:
Objective: To establish a RP-HPLC method for the determination of emodin and chrysophanol in ZhanJing tincture. Method: Symmetry C18chromatographic column (5.0μm) was adopted, methanol-0.1% phosphoric acid (85∶15)as the mobile phase, flow rate was 0.8 ml·min-1; the column temperature was 20℃ , the detection wavelength was 423nm. Result: Emodin and chrysophanol showed better linear relationship in the concentration range of 10.0 to 30.0μg/mL and peak area (r=0.999 9, 0.999 7); average ecovery rates were 101.35% and 98.25% respectively (RSD=2.26%, 1.17%). Conclusion: The method, rapid, sensitive and specific, could be used for the content determination of ZhanJing tincture.

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备注/Memo

备注/Memo:
收稿日期:2012-10-27 * 基金项目:甘肃省科学事业费资助项目(编号 GS061-C33-29) 作者简介:郭钰龙(1970—),男,主管中药师。研究方向:中药制剂研究与开发。 △通讯作者:杨锡仓(1950—),男,主任中药师,首届全国老中医药专家学术经验继承人(1994年出师),现担任甘肃省老中医药专家学术经验继承工作指导老师,全国中医药院校中药标本馆专业委员会理事,甘肃省中医药学会中药分会副理事长,兰州市药学会理事,《中华中西医结合杂志》编委。研究方向:中药材炮制、鉴定及中药制剂研究与开发。
更新日期/Last Update: 2013-05-15