知柏地黄丸对性早熟模型大鼠FSH、T水平的影响*-《西部中医药》

文章信息/Info

Title:: Action of ZhiBai DiHuang Pill on the Levels of FSH and T of Rats with Sexual Precocity

Author(s):: WU Liping1, LI Yuxia1, SHANG Jing2, LV Xiaowu2; 1 University Hospital of Gansu Traditional Chinese Medicine, Lanzhou 730020, China; 2 Gansu University of Traditional Chinese Medicine

Keywords:: sexual precocity; ZhiBai DiHuang pill; follicle-stimulating hormone; serum testosterone; rat

摘要:: 目的：探讨知柏地黄丸对雌性真性性早熟大鼠垂体分泌的卵泡刺激素（FHS）、性腺分泌的睾酮（T）水平的影响。方法：将26日龄雌性SPF级SD大鼠50只随机分为5组，每组10只。采用N-甲基-D-天冬氨酸（NMDA）方法诱发建立性早熟动物模型，分别用放免法测定血清FSH、T水平。结果：模型组与空白组、知柏地黄丸大、中剂量组大鼠血清FSH及T水平比较，差异均有统计学意义（P＜0.05），说明知柏地黄丸可降低性早熟大鼠血清FSH，T水平；小剂量组与大、中剂量组血清FSH，T水平比较，差异有统计学意义（P＜0.05），说明知柏地黄丸调节性早熟大鼠血清FSH，T水平存在量效关系。结论：知柏地黄丸可抑制NMDA诱发雌性大鼠性早熟的发生及模型大鼠血清性激素FSH，T水平的表达。推测知柏地黄丸对模型大鼠下丘脑－垂体－性腺轴有调节作用。

Abstract:: Objective: To discuss the action of ZhiBai DiHuang pill on testosterone (T) and follicle-stimulating hormone (FSH) of rats suffering from sexual precocity. Method: All 50 rats were randomized into five groups, ten rats each group. Animal models were established by the method of N-methyl-D-aspartate (NMDA). The levels of T and FSH were detected through radioimmunoassay respectively. Result: The difference had statistical meaning in the levels of FSH and T from the comparison between model group and blank group, high dosage and low dosage groups of ZhiBai DiHuang pill(P＜0.05). It demonstrated that ZhiBai DiHuang pill could lower the levels of T and FSH; the difference had statistical meaning in the levels of T and FSH when low dosage group was compared with high and moderate dosage groups of ZhiBai DiHuang pill(P＜0.05), it presented that ZhiBai DiHuang pill and regulating the levels of T and FSH showed the dose-effect relationship. Conclusion: ZhiBai DiHuang pill could inhibit the incidence of sexual precocity in female rat caused by NMDA and the expressions of T and FSH of rat model. We speculate that ZhiBai DiHuang pill could regulate hypothalamic-pituitary-gonadal axis of model rat.