[1]赵弼洲,田佳灵,余占海△,等.淫羊藿苷对人牙周膜细胞OCN表达水平的影响[J].西部中医药,2014,27(01):26-28.
　ZHAO Bizhou,TIAN Jialing,YU Zhanhai,et al.Effects of Icariin on the Expression of OCN in Human Periodontal Ligament Cells[J].Western Journal of Traditional Chinese Medicine,2014,27(01):26-28.
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淫羊藿苷对人牙周膜细胞OCN表达水平的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年01期
页码:
26-28
栏目:
出版日期:
2014-01-14

文章信息/Info

Title:
Effects of Icariin on the Expression of OCN in Human Periodontal Ligament Cells
文章编号:
1004-6852(2014)01-0026-03
作者:
赵弼洲1，田佳灵1，余占海2△，胡永寿1，马海冰1
1 甘肃省中医院口腔科，甘肃 兰州 730050； 2 兰州大学口腔医学院
Author(s):
ZHAO Bizhou1, TIAN Jialing1, YU Zhanhai2△, HU Yongshou1, MA Haibing1
1 Department of Stomatology in Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050, China; 2 School of Stomatology in Lanzhou University
关键词:
淫羊藿苷; 人牙周膜细胞; 骨向分化; 骨钙素
Keywords:
icariin;  human periodontal ligament cells;  osteogenic differentiation;  osteocalcin
分类号:
R276.8
文献标志码:
A
摘要:
目的：探讨淫羊藿苷对人牙周膜细胞骨钙素(OCN)表达水平的影响，为淫羊藿苷治疗牙周病提供依据。方法：体外培养人牙周膜细胞，矿化诱导条件下将第3代细胞与10～0.001 μg/mL6个浓度的淫羊藿苷作用，Elisa测定OCN表达水平。结果：人牙周膜细胞在矿化诱导条件下与不同浓度淫羊藿苷作用72小时后，1～0.01 μg/mL组的OCN表达水平均较对照组显著增加(P＜0.05)；10 μg/mL组OCN表达水平较对照组低，差异有统计学意义(P＜0.05)。结论：淫羊藿苷在一定浓度范围内可促进人牙周膜细胞骨向分化。
Abstract:
Objective: To provide evidence for the application of icariin to periodontal disease by exploring the effects of icariin on the expressions of osteocalcin (OCN) in human periodontal ligament cells. Methods: Human periodontal ligament cells were cultured in vitro. Under the conditions of induced mineralization, the cells of generation three were dealt with icariin in different concentrations(10～0.001μg/mL), the levels of OCN were detected by Elisa. Results: Compared with the control group, the expressions of OCN in the group of 1~0.01 μg/mL were increased remarkably when human periodontal ligament cells were dealt with icariin in different concentrations after 72 hours (P＜0.05); the expressions of OCN in the group of 10μg/mL were less than the control group, the difference had statistical meaning(P＜0.05). Conclusion: icariin in a certain concentrations could promote osteogenic differentiation of human periodontal ligament cells.

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备注/Memo

备注/Memo:
收稿日期：2013-01-20作者简介：赵弼洲(1985—)，男，硕士学位，医师。研究方向：口腔疾病的天然药物治疗。△通讯作者：余占海(1964—)，男，博士学位，教授。研究方向：口腔疾病的天然药物治疗。
更新日期/Last Update: 2014-01-15