[1]王秋兰,朱建坤,韩涛,等.白花蛇舌草总黄酮对苯并(a)芘致实体瘤小鼠IL-2和IL-6的影响[J].西部中医药,2015,28(05):14-17.
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白花蛇舌草总黄酮对苯并(a)芘致实体瘤小鼠IL-2和IL-6的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
28
期数:
2015年05期
页码:
14-17
栏目:
方药·药理研究
出版日期:
2015-05-15

文章信息/Info

文章编号:
1004-6852(2015)05-0014-04
作者:
王秋兰朱建坤韩涛林丽
甘肃中医学院,甘肃 兰州730000
关键词:
肿瘤白花蛇舌草总黄酮苯并(a)芘IL-2IL-6
分类号:
R378.7
文献标志码:
A
摘要:
目的:通过IL-2、IL-6的测定,探索白花蛇舌草总黄酮抑制肿瘤的机理及苯并(a)芘对机体免疫功能的影响。方法:将SPF级昆明种小鼠60只随机分为对照组20只及苯并(a)芘实验组40只,再将苯并(a)芘实验组随机分为苯并(a)芘10 mg/kg组、苯并(a)芘20 mg/kg组,每组20只,雌雄各半分笼饲养,并以二甲亚砜溶解苯并(a)芘,然后分别以苯并(a)芘10 mg/kg和20 mg/kg,腹腔注射,1次/d,连续注射10次,以DMSO 100 μL/只腹腔注射作为对照,2个月后将苯并(a)芘10 mg/kg组、苯并(a)芘20 mg/kg组再分为白花蛇舌草总黄酮高、低剂量组2组(乙醇回流法提取白花蛇舌草总黄酮),每组10只,分别给予白花蛇舌草流浸膏20 g/kg、10 g/kg灌胃,每天一次,连续给药2周后取材。采用ELISA法测定血清中IL-2及IL-6;取肝、脾、肾、胰、胃肠、肺等器官,常规包埋,切片,HE染色,显微镜下观察。结果:IL-2浓度,对照组、苯并(a)芘10 mg/kg组、苯并(a)芘20 mg/kg组分别为(360±15.92) ng/L、(92.59±6.12)ng/L、(83.84±5.89)ng/L,实验组明显低于对照组;蛇草总黄酮高剂量组高于低剂量组。IL-6浓度,对照组为(32.65±5.32)pg/mL,苯并(a)芘10 mg/kg组、20 mg/kg组分别为(21.78±4.62) pg/ml、(24.54±9.89 )pg/mL,与对照组比较差异无显著性;蛇草总黄酮高、低剂量组比较差异无显著性。苯并(a)芘对肝脏、胃、肾脏有明显损害,癌前病变率与对照组比较均有显著差异;对胃的损害,苯并(a)芘20 mg/kg组明显高于苯并(a)芘10 mg/kg组,呈明显的量效关系;实验组肺脏、胰腺均未见明显损害。结论:实验各组IL-2明显低于对照组,提示苯并(a)芘对机体的细胞免疫有明显抑制作用;苯并(a)芘对肝脏、胃和肾脏均有明显损害;胃癌发生率呈明显的量效关系。白花蛇舌草总黄酮高剂量组IL-2浓度明显高于低剂量组,而IL-6浓度无显著差异,提示白花蛇舌草总黄酮有一定的细胞免疫调节功能,可能通过调节T细胞功能,对抗化疗药物的骨髓抑制而实现。

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备注/Memo

备注/Memo:
*基金项目:2010年甘肃省科技支撑计划-社会发展类(编号1011FKCA135);甘肃省高等学校基本科研业务费项目(编号2012-5)作者简介:王秋兰(1970—),女,硕士学位,副教授。研究方向:肿瘤学。
更新日期/Last Update: 2015-05-15