[1]刘天易.芍药苷对大鼠脑缺血再灌注损伤细胞凋亡的影响及其作用机制[J].西部中医药,2016,29(05):8-11.
 LIU Tianyi.The Effects of Peoniflorin on Cellular Apoptosis of the Rats with Cerebral Ishchemia Reperfusion Injury and Its Mechanism[J].Western Journal of Traditional Chinese Medicine,2016,29(05):8-11.
点击复制

芍药苷对大鼠脑缺血再灌注损伤细胞凋亡的影响及其作用机制()
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年05期
页码:
8-11
栏目:
出版日期:
2016-05-15

文章信息/Info

Title:
The Effects of Peoniflorin on Cellular Apoptosis of the Rats with Cerebral Ishchemia Reperfusion Injury and Its Mechanism
文章编号:
1004-6852(2016)05-0008-04
作者:
刘天易
山东中医药大学附属医院药学部,山东 济南 250000
Author(s):
LIU Tianyi
Pharmaceutical Department of Affiliated Hospital to Shandong University of Traditional Chinese Medicine, Jinan 250000, China
关键词:
脑缺血再灌注细胞凋亡芍药苷
Keywords:
cerebral ischemia reperfusion cellular apoptosis peoniflorin
分类号:
R285.5
文献标志码:
A
摘要:
目的:探讨芍药苷对大鼠脑缺血再灌注损伤细胞凋亡的影响及其作用机制。方法:选择健康雄性SD大鼠80只,随机分为假手术组、模型组、芍药苷组及尼莫地平组各20只,建立大鼠脑缺血再灌注损伤模型,观察各组大鼠神经行为学评分及脑梗死面积,TUNEL检测大鼠神经细胞凋亡情况,免疫组化法检测大鼠大脑皮层中Bcl-2和Bax蛋白表达情况。结果:假手术组大鼠未表现出神经功能缺失症状,模型组大鼠神经行为学评分及脑梗死面积高于假手术组,芍药苷组及尼莫地平组均低于模型组,差异具有统计学意义(P<0.05);假手术组几乎未见神经细胞凋亡,模型组TUNEL阳性细胞明显多于假手术组,芍药苷组及尼莫地平组细胞凋亡个数及阳性率均低于模型组,其差异具有统计学意义(P<0.05);假手术组大鼠大脑皮质中Bcl-2及Bax的灰度值均高于模型组,芍药苷组及尼莫地平组大鼠Bcl-2灰度值较模型组降低,而Bax灰度值较模型组明显升高,2组Bcl-2/ Bax灰度值较模型组下降,其差异具有统计学意义(P<0.05)。结论:芍药苷可通过抑制Bax的表达,上调Bcl-2的表达来抑制神经细胞凋亡,从而保护神经功能,减轻脑梗死。
Abstract:
Objective: To investigate the effects of peoniflorin on cellular apoptosis of the rats with cerebral ischemia reperfusion injury and its mechanism. Methods: Eighty SD rats were randomized into sham operation group, the model group, peoniflorin group and nimodipine group, 20 cases in each group, the rat model with cerebral ischemia reperfusion injury was established, neurological behavior score and infarct area of the rats in different groups were observed, neural cell apoptosis of the rats were detected by TUNEL, the expressions of Bcl-2 and Bax protein were measured by immunohistochemical method. Results: The rats in the sham operation group didn't manifest the symptoms of nerve function impairment, the model group was higher than the sham operation group in neurological behavior score and infarct area obviously, peoniflorin group and nimodipine group were lower than the model group notably, and the difference showed statistical meaning (P<0.05); no nerve cellular apoptosis had been seen in the sham operation group, the model group was more than the sham operation group in TUNEL positive cells, peoniflorin group and nimodipine group were lower than the model group in the numbers of apoptotic cells and positive rates significantly, and the difference showed statistical meaning (P<0.05); gray levels of Bcl-2 and Bax in the sham operation group were higher than these of the model group, gray level of Bcl-2 decreased in peoniflorin group and nimodipine group compared with the model group, while Bax gray level raised obviously in both groups compared with the model group, and the difference demonstrated statistical meaning (P<0.05). Conclusion: Peoniflorin could inhibit the apoptosis of nerve cells by inhibiting the expressions of Bax and up regulating Bcl-2 expressions, thereby to protect nerve function and alleviate cerebral infarction.

相似文献/References:

[1]邢涛,董林△,魏国俊,等.骨刺消巴布剂对大鼠急性软组织损伤细胞凋亡的影响*[J].西部中医药,2013,26(12):17.
 XING Tao,DONG Lin,WEI Guojun,et al.Effects of GuCiXiao Cataplasm on Cellular Apoptosis of Rat with Acute Soft Tissue Injury[J].Western Journal of Traditional Chinese Medicine,2013,26(05):17.
[2]葛世杰,刘晓婷,张沂,等.疏风宣肺方与解表清里方对流感病毒H1N1感染的A549细胞凋亡的影响[J].西部中医药,2015,28(05):7.
[3]丁明明,吴勉华△.中药对肺癌细胞凋亡诱导基因表达的影响[J].西部中医药,2015,28(05):142.
[4]黄舒然,王瑞平△,邹 玺,等.蜂毒素对人胃癌细胞株BGC-823凋亡及其细胞周期的影响*[J].西部中医药,2012,25(10):15.
 HUANG Shu-ran,WANG Rui-ping,ZOU Xi,et al. Effects of Melittin on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line BGC-823[J].Western Journal of Traditional Chinese Medicine,2012,25(05):15.
[5]严亚锋,白海侠.消栓通络胶囊对急性脑缺血再灌注大鼠脑组织TNF-α和IL-1β水平的影响[J].西部中医药,2013,26(09):23.
 YAN Yafeng,BAI Haixia.Effects of XiaoShuangTongLuo Capsule on TNF-α and IL-1β in Cerebral Tissue of Rats with Acute Cerebral Ischemia-reperfusion Injury[J].Western Journal of Traditional Chinese Medicine,2013,26(05):23.
[6]苏韫,刘永琦,颜春鲁,等.黄芪多糖对脑缺血再灌注大鼠脑组织Bcl-2、Bax蛋白及NO含量的影响[J].西部中医药,2016,29(03):10.
 SU Yun,LIU Yongqi,YAN Chunlu,et al.Influence of Astragalus Polysaccharides on the Contents of Bcl-2, Bax Protein and NO in the Brain Tissue of the Rats with Cerebral Ischemia Reperfusion[J].Western Journal of Traditional Chinese Medicine,2016,29(05):10.
[7]谢守嫔,罗佳,李海龙,等.当归含药血清对Aβ25-35诱导PC12细胞凋亡的保护作用研究[J].西部中医药,2015,28(06):13.[doi:2015/6/16 0:00:00]
[8]梁涢.四物汤对脑缺血再灌注小鼠海马结构及脑组织SOD和GSH-PX活力的影响[J].西部中医药,2017,30(02):10.
 The Effects of SiWu Decoction on Hippocampus and Activity of Brain Tissue SOD and GSH-PX in Mice with Cerebral Ischemia-reperfusion.The Effects of SiWu Decoction on Hippocampus and Activity of Brain Tissue SOD and GSH-PX in Mice with Cerebral Ischemia-reperfusion[J].Western Journal of Traditional Chinese Medicine,2017,30(05):10.
[9]张喜平,苏艳,王杏敏.复方三七散外敷对静脉留置针所致静脉内皮细胞凋亡的保护作用[J].西部中医药,2016,29(02):16.
 ZHANG Xiping,SU Yan,WANG Xingmin.Effects of Compound SanQi Powder for External Use on Venous Indwelling Needle-induced Cellular Apoptosis in Venous Endothelium[J].Western Journal of Traditional Chinese Medicine,2016,29(05):16.
[10]李盛华,吴献毅,周明旺,等.细胞凋亡在膝骨性关节炎半月板损伤中的作用研究[J].西部中医药,2018,31(02):131.
 LI Shenghua,WU Xianyi,ZHOU Mingwang,et al.Research on the Roles of Cellular Apoptosis Played in Meniscus Injury of the Patients with Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2018,31(05):131.

备注/Memo

备注/Memo:
收稿日期:2015-02-23作者简介:刘天易(1983—),男,硕士学位,主管药师。研究方向:中药学及中药药理学。
更新日期/Last Update: 2016-05-15