[1]杨有林,齐武强.半夏多糖提取工艺优化及其抗氧作用研究[J].西部中医药,2016,29(07):37-41.
　YANG Youlin,QI Wuqiang.Study on Antioxidant Activity of Polysaccharide from BanXia and Extraction Process Optimization[J].Western Journal of Traditional Chinese Medicine,2016,29(07):37-41.
点击复制

半夏多糖提取工艺优化及其抗氧作用研究
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年07期
页码:
37-41
栏目:
出版日期:
2016-07-15

文章信息/Info

Title:
Study on Antioxidant Activity of Polysaccharide from BanXia and Extraction Process Optimization
文章编号:
1004-6852(2016)07-0037-05
作者:
杨有林1，齐武强2
1 宝鸡市妇幼保健院药剂科，陕西 宝鸡 721000； 2 宝鸡市中医医院药剂科
Author(s):
YANG Youlin1, QI Wuqiang2
1 Pharmacy Department of Baoji Municipality Maternity and Child-care Hospital, Baoji 721000, China;2 Pharmacy Department of Baoji Municipality TCM Hospital
关键词:
半夏; 多糖; 复合酶; 响应面; 抗氧化
Keywords:
BanXia;  polysaccharide;  complex enzyme;  response surface;  antioxidation
分类号:
R283
文献标志码:
A
摘要:
目的：通过响应面法优化复合酶提取半夏多糖的工艺，并评价其抗氧化活性。方法：以半夏多糖得率为响应值，以液料比、酶解温度、酶解时间、复合酶(木瓜蛋白酶、纤维素酶、果胶酶的质量比为2∶2∶1)添加量为试验因素，采用响应面法建立数学模型，优化提取条件；体外抗氧化活性考察半夏多糖对DPPH和O2-·自由基的清除能力。结果：通过二次回归模型响应面分析，酶解温度、时间、复合酶添加量、液料比4因素对半夏多糖得率的影响依次减弱；酶解温度与时间优化为54℃和57分钟，复合酶添加量为7.2 mg/mL，液料比例为27∶1 mL/g，在此最佳工艺条件下半夏多糖得率为27.98%，模型方程理论预测值为28.32%，两者相对误差小于5%。半夏多糖具有较强的抗氧化活性，对DPPH和O2-·自由基的半数抑制浓度分别为0.987 mg/mL、1.309 mg/mL，但与维生素C比较，抗氧化活性较弱。结论：采用响应面法优化得到了半夏多糖的最佳复合酶提取条件，且半夏多糖有一定的体外抗氧化作用。
Abstract:
Objective: To optimize complex enzymatic extraction technology of polysaccharide from BanXia (Pinellia ternata) by response surface method, and evaluate its antioxidant activity. Methods: Polysaccharide yield of BanXia was taken as the response, liquid to material ratio, enzymatic hydrolysis temperature, enzymatic hydrolysis time and complex enzymes (mass ratios of papain, cellulose and pectase were 2:2:1) as experimental factors, mathematical model was established by adopting response surface method to optimize extraction conditions; antioxidant activity in vitro was used to investigate the clearance ability of polysaccharide extracted from BanXia to DPPH and O2-·radical. Results: By analyzing with two regression model response surface, the influences of enzymatic hydrolysis temperature, enzymatic hydrolysis time, additive amount of complex enzymes and liquid to material ratio became attenuated gradually; enzymatic hydrolysis temperature and enzymatic hydrolysis time were optimized as 54℃ and 57 minutes, additive amount of complex enzymes were 7.2 mg/mL, liquid to material ratio was 27:1 mL/g, under the best extraction technology, polysaccharide yield of BanXia was 27.98%, model equation theoretical prediction value was 28.32%, and relative error between both was less than 5%. Polysaccharide from BanXia presented stronger antioxidant activity, half maximal inhibitory concentrations of DPPH and O2-radical were 0.987 mg/mL and 1.309 mg/mL, but its antioxidant activity was weaker compared with vitamin C. Conclusion: The best complex enzymatic extraction technology of polysaccharide from BanXia is obtained by adopting response surface method, and the polysaccharide from BanXia shows antioxidant activity in vitro to a certain degree.

相似文献/References:

[1]张艳.论半夏安神治失眠[J].西部中医药,2012,25(11):43.
　ZHANG Yan.On Tranquilizing of BanXia for Insomnia[J].Western Journal of Traditional Chinese Medicine,2012,25(07):43.
[2]李鸿飞,张凤雪,李柯翱,等.黄精膏中水溶性总糖与多糖的测定*[J].西部中医药,2015,28(01):16.
[3]李成义,杨苗苗,张樱山,等.甘肃不同产地板蓝根中多糖含量分析*[J].西部中医药,2015,28(02):5.
[4]马趣环,石晓峰△,王新娣,等.苯酚硫酸法与蒽酮硫酸法测定糙叶败酱多糖含量的比较研究[J].西部中医药,2015,28(12):30.
[5]王彩琴,杜娟,王一敏,等.甘肃半夏资源及研究现况[J].西部中医药,2015,28(03):22.
[6]李广雷,杨薇,林青华,等.枸杞子的提取工艺研究[J].西部中医药,2015,28(08):18.
[7]王岳峰,丁昭利.峨参多糖含量测定与抗氧化作用的研究[J].西部中医药,2015,28(08):21.
[8]李万军,马新焕,王建良.半夏的药理作用[J].西部中医药,2012,25(09):129.
　LI Wan-jun,MA Xin-huan,WANG Jian-liang.Pharmacological Action of BanXia[J].Western Journal of Traditional Chinese Medicine,2012,25(07):129.
[9]刘世军,李瑞,杨旭,等.大枣多糖的纯化工艺研究[J].西部中医药,2017,30(03):36.
　LIU Shijun,LI Rui,et al.Study on the Purification Technology of Jujube Polysaccharide[J].Western Journal of Traditional Chinese Medicine,2017,30(07):36.
[10]罗林云.李可中医药学术流派附子半夏配伍临床应用特点[J].西部中医药,2017,30(04):70.
　LUO Linyun.Discussion on Clinical Application Features of FuZi Compatible with BanXia Based on LI Ke′s Academic School of Chinese Medicine[J].Western Journal of Traditional Chinese Medicine,2017,30(07):70.

备注/Memo

备注/Memo:
收稿日期：2015-03-17 作者简介：杨有林(1974—)，男，主管中药师。研究方向：中药饮片的临床应用。
更新日期/Last Update: 2016-07-15