[1]古秋莉,刘效栓,李喜香,等.HPLC法测定养阴生肌妇炎栓中龙胆苦苷的含量[J].西部中医药,2018,31(05):17-19.
　GU Qiuli,LIU Xiaoshuan,LI Xixiang,et al.Content Determination of Gentiamarin in YangYin ShengJi FuYan Suppository by HPLC[J].Western Journal of Traditional Chinese Medicine,2018,31(05):17-19.
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HPLC法测定养阴生肌妇炎栓中龙胆苦苷的含量()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年05期
页码:
17-19
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Content Determination of Gentiamarin in YangYin ShengJi FuYan Suppository by HPLC
文章编号:
1004-6852(2018)05-0017-03
作者:
古秋莉1，刘效栓1，李喜香1，郭敏2
1 甘肃省中医院，甘肃 兰州 730050； 2 甘肃中医药研究院
Author(s):
GU Qiuli1, LIU Xiaoshuan1, LI Xixiang1, GUO Min2
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China;2 Gansu Provincial Academy of Chinese Medicine
关键词:
HPLC; 养阴生肌妇炎栓; 龙胆苦苷
Keywords:
HPLC;  YangYin ShengJi FuYan suppository;  gentiamarin
分类号:
R282
文献标志码:
A
摘要:
目的：建立养阴生肌妇炎栓中龙胆苦苷的含量测定方法。方法：采用高效液相色谱法，色谱柱为Shim-pack VP-ODS(150×4.6 mmI.D.)，以水-乙腈(80∶20)为流动相，流速为1.0 mL/min，检测波长254 nm，柱温30℃进行检测。结果：龙胆苦苷的线性范围为：20.0～110.0 μg/mL，线性方程为：Y=12519X+23859，r=0.999 8；平均加样回收率为98.10%(RSD=0.41%)。结论：该法稳定、快速、简便，结果准确、可靠，可用于养阴生肌妇炎栓中龙胆苦苷的含量测定。
Abstract:
Objective: To establish the methods for content determination of gentiamarin in YangYin ShengJi FuYan suppository. Methods: High performance liquid chromatography was used, they were detected when chromatographic column was Shim-pack VP-ODS (150×4.6 mmI. D.), mobile phase: water -acetonitrile (80∶20), flow rate: 1.0 mL/min, detection wavelength: 254nm, column temperature was 30 ℃. Results: Linearity range of gentiamarin was 20.0～110.0 μg/mL, linear equation was Y=12519X+23859, r=0.999 8; average recovery rate was 98.10% (RSD=0.41%). Conclusion: The method, stable, rapid, convenient, accurate and reliable, could be used for content determination of gentiamarin in YangYin ShengJi FuYan suppository.

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备注/Memo

备注/Memo:
收稿日期：2017-02-20 *基金项目：甘肃省自然科学基金(编号1606RJZA149)。 作者简介：古秋莉(1964—)，女，副主任药师。研究方向：医院药学。
更新日期/Last Update: 2018-05-15