[1]权彦,李小蓉,赵忠孝,等.木犀草素对急性酒精性肝损伤的保护作用[J].西部中医药,2018,31(06):20-23.
　QUAN Yan,LI Xiaorong,ZHAO Zhongxiao,et al.Protective Effects of Luteolin against Acute Liver Injury Induced by Alcohol[J].Western Journal of Traditional Chinese Medicine,2018,31(06):20-23.
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木犀草素对急性酒精性肝损伤的保护作用()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年06期
页码:
20-23
栏目:
出版日期:
2018-06-15

文章信息/Info

Title:
Protective Effects of Luteolin against Acute Liver Injury Induced by Alcohol
文章编号:
1004-6852(2018)06-0020-04
作者:
权彦，李小蓉，赵忠孝，刘靖丽，张朋
陕西中医药大学，陕西 咸阳 712046
Author(s):
QUAN Yan, LI Xiaorong, ZHAO Zhongxiao, LIU Jingli, ZHANG Peng
Shaanxi University of Chinese Medicine, Xianyang 712046, China
关键词:
木犀草素; 酒精性肝损伤; 抗氧化
Keywords:
luteolin;  alcohol liver injury;  antioxidation
分类号:
R575
文献标志码:
A
摘要:
目的：观察木犀草素对酒精所致小鼠急性肝损伤的保护作用。方法：将120只雄性小鼠随机分成空白对照组、模型对照组、阳性对照组［联苯双酯150 mg/(kg·d)、木犀草素低剂量组（25 mg/kg)、木犀草素中剂量组（50 mg/kg)、木犀草素高剂量组（100 mg/kg)］，采用酒精灌胃方法分别建立小鼠醉酒和急性肝损伤模型，观察木犀草素对小鼠生理状态、肝指数及血清中丙氨酸氨基转移酶（ALT/GPT)、天冬氨酸氨基转移酶（AST/GOT)、甘油三酯（TG)和胆固醇(TC)的影响，同时通过苏木精-伊红染色(HE染色)光学显微镜观察小鼠肝组织病理学改变及试剂盒检测超氧化物歧化酶(SOD)、丙二醛（MDA)变化，考察木犀草素对急性酒精性肝损伤的保护的作用及可能机制。结果：与模型组比较，木犀草素可以改善小鼠醉酒状态，降低小鼠肝指数，给药组血清学指标ALT/GPT、AST/GOT、TG、TC降低，病理组织学检查结果表明木犀草素对小鼠肝小叶结构、肝细胞坏死和变性及炎细胞浸润等方面均有改善作用，且肝组织内SOD升高，MDA降低。结论：木犀草素具有防醉和缓解酒精所致的肝损伤的作用，其机制可能与降低自由基生成、减轻脂质过氧化有关。
Abstract:
Objective: To observe the protective effects of luteolin against acute liver injury(ALI) induced by alcohol of the mice. Methods: All 120 mice were randomly allocated to blank control group, model control group, positive control groups [biphenyl dicarboxylate 150 mg/( kg·d) , low dosage group of leteolin (25 mg/kg), moderate dosage group of luteolin(50 mg/kg) and high dosage group of leteolin (100 mg/kg)］. Drunk mice model and ALI mice model were established respectively by using lavage of alcohol, to observe the effects of luteolin on physiological state, liver index, ALT/GPT, AST/GOT, TG and TC, simultaneously, the changes of SOD and MDA were detected by kit, pathological changes of liver tissue in the mice were observed by HE staining method under light microscope, the protective effects of luteolin against ALI induced by alcohol and the potential mechanism were investigated.Results: Compared with the model group, luteolin could improve the drunk state of the mice, decrease liver index of the mice, serological indexes in the medication groups including ALT/GPT, AST/GOT, TG and TC decreased, the results of histopathologic examination demonatrated that luteolin could improve hepatic lobule structure, necrosis, degeneration of liver cells, inflammatory cell infiItration of the mice, SOD raised and MDA lowered in liver tissue. Conclusion: Luteolin possesses the effects of preventing drunkness and relieving alcohol-induced ALI, and its mechanism might be related to decreasing free radical formation and relieving lipid peroxidation.

相似文献/References:

[1]唐莉,姚婧,郭立中△.解酒护肝方对不同体质大鼠酒精性肝损伤的保护作用[J].西部中医药,2014,27(02):17.
　TANG Li,YAO Jing,GUO Lizhong.Study on the Protection of JieJiu HuGan Prescription against Alcoholic Liver Injury of Rats in Different Constitutions[J].Western Journal of Traditional Chinese Medicine,2014,27(06):17.

备注/Memo

备注/Memo:
收稿日期：2017-12-05 *基金项目：陕西省社会发展科技攻关项目（编号2016SF-399）；2015年国家级大学生创新创业训练计划项目（编号1903）；陕西中医药大学青年基金项目（编号2015QN24）；咸阳市2016年科学技术研究计划项目（编号2016k02-84）；2016年陕西省大学生创新创业训练计划项目（编号2179）。 作者简介：权彦(1976—)，男，硕士学位，讲师。研究方向：中草药分离提取。
更新日期/Last Update: 2018-06-15