[1]李亮,姬艳苏△.黄芪总皂苷对心力衰竭大鼠JAK2和STAT3的影响[J].西部中医药,2018,31(09):17-20.
　LI Liang,JI Yansu.Effects of Total Saponins of HuangQi on JAK2/STAT3 of the Rats with Heart Failure[J].Western Journal of Traditional Chinese Medicine,2018,31(09):17-20.
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黄芪总皂苷对心力衰竭大鼠JAK2和STAT3的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年09期
页码:
17-20
栏目:
出版日期:
2018-09-15

文章信息/Info

Title:
Effects of Total Saponins of HuangQi on JAK2/STAT3 of the Rats with Heart Failure
文章编号:
1004-6852(2018)09-0017-04
作者:
李亮1，姬艳苏2△
1 天津医科大学总医院，天津 300052； 2 武警后勤学院附属医院
Author(s):
LI Liang1, JI Yansu2△
1 General Hospital, Tianjin Medical University, Tianjin 300052, China; 2 Affiliated Hospital of Logistics University of Chinese People′s Armed Police Forces
关键词:
慢性心力衰竭; JAK2/STAT3; 黄芪总皂苷
Keywords:
chronic heart failure;  JAK2/STAT3;  total saponins of HuangQi
分类号:
R563
文献标志码:
A
摘要:
目的：探讨黄芪总皂苷对心肌梗死后心力衰竭大鼠心肌酪氨酸激酶2/信号传导及转录激活因子3(JAK2/STAT3)mRNA和蛋白表达的影响。方法：复制心肌梗死后慢性心力衰竭大鼠模型，采用酶联免疫吸附法(ELISA)检测血清氮末端-前体脑钠肽(NT-proBNP)含量变化，Ⅷ因子染色法观察心肌微血管数目，实时荧光定量PCR技术观察羧基端活性段脑钠肽(BNP)、JAK2和STAT3 mRNA水平，并用蛋白免疫印迹(Western-Blot)技术观察心肌JAK2和STAT3蛋白水平的表达以及黄芪总皂苷的干预作用。结果：黄芪总皂苷能增加心肌微血管密度，下调心肌梗死后心力衰竭大鼠心肌组织JAK2在mRNA水平的表达，上调STAT3 mRNA和蛋白水平。结论：调节JAK2/STAT3可能是黄芪总皂苷抗心力衰竭，改善心肌供血，促进血管新生的机制之一。
Abstract:
Objective: To discuss the effects of total saponins of HuangQi(Astragalus mongholicus) on JAK2/STAT3 mRNA and protein expressions of the rats with heart failure after myocardial infarction. Methods: Rat model with chronic heart failure (CHF) after myocardial infarction was duplicated, the contents of NT-proBNP were detected by ELISA method, the number of myocardial microvessels was observed by VIII factor staining, the levels of BNP, JAK2 and STAT3 mRNAwere observed by real-time fluorescence quantitative PCR technology, the intervention effects of total saponins of HuangQi and the expressions of JAK2 and STAT3 protein were observed by Western-Blot skills. Results: Total saponins of HuangQi could increase myocardial microvessel density, decrease the expressions of JAK2 mRNA in myocardial tissue of the rats suffering CHF after myocardial infarction, and raise the levels of STAT3 mRNA and protein. Conclusion: Regulating JAK2/STAT3 might be one of the mechanisms of total saponins of HuangQi fighting against heart failure, improving myocardial blood supply and promoting the angiogenesis.

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备注/Memo

备注/Memo:
收稿日期：2018-01-20 *基金项目：天津市应用基础与前沿技术研究计划(编号14JCQNJC13600)。 作者简介：李亮(1980—)，男，硕士学位，主管药师。研究方向：中药药理学。 △通讯作者：姬艳苏(1981—)，女，硕士学位，主管药师。研究方向：中药药理学。
更新日期/Last Update: 2018-09-15