[1]王玲,吴秋枫△.灯盏乙素对糖尿病肾病大鼠的保护作用[J].西部中医药,2018,31(09):30-33.
　WANG Ling,WU Qiufeng.Safety Action of Scutellarin on the Rats with Diabetic Nephropathy[J].Western Journal of Traditional Chinese Medicine,2018,31(09):30-33.
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灯盏乙素对糖尿病肾病大鼠的保护作用()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年09期
页码:
30-33
栏目:
出版日期:
2018-09-15

文章信息/Info

Title:
Safety Action of Scutellarin on the Rats with Diabetic Nephropathy
文章编号:
1004-6852(2018)09-0030-04
作者:
王玲，吴秋枫△
武汉科技大学附属天佑医院中医科，湖北 武汉 430064
Author(s):
WANG Ling, WU Qiufeng△
TCM Department, Tianyou Hospital Affiliated to Wuhan University of Science & Technology, Wuhan 430064, China
关键词:
糖尿病肾病; 肾脏; 核转录因子E2相关因子2; 血红素加氧酶1; 灯盏乙素
Keywords:
diabetic nephropathy;  kidney;  nuclear factor E2 related factor 2;  heme oxygenase-1;  scutellarin
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨灯盏乙素(SC)对糖尿病肾病(DN)大鼠的保护作用及其分子机制。方法：将雄性SD大鼠50只随机分为5组各10只，正常对照组给予普通饲料喂养，DN模型组以及SC低剂量［25 mg/(kg·d)］组、中剂量［50 mg/(kg·d))组以及高剂量［100 mg/(kg·d)］组给予高脂饲料喂养，4周后进行链脲佐菌素( STZ，60 mg/kg)注射造模，造模成功后SC各剂量组灌胃SC，DN模型组灌胃相同体积生理盐水，4周后处死大鼠，收集血清和肾脏组织进行相关因子检测。结果：与正常对照组比较，DN模型组大鼠血清中血尿素氮(BUN)、血肌酐(Scr)、葡萄糖、胰岛素、胰岛素抵抗指数(HOMA-IR)、丙二醛(MDA)、甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量升高(P＜0.05)，SC治疗后各指标下降(P＜0.05)，且具有剂量依赖性；抗氧化因子超氧化物歧化酶(SOD)和谷胱甘肽(GSH)含量以及核转录因子E2相关因子2(Nrf2)和血红素加氧酶1(HO-1)的mRNA和蛋白表达水平在DN模型组中降低，经SC治疗后下降明显(P＜0.05)，且具有剂量依赖性。结论：SC可改善DN大鼠肾功能、降低体内IR与脂质沉积，通过调节 Nrf2 /HO-1信号通路减轻氧化应激反应从而缓解DN。
Abstract:
Objective: To investigate the safety action of scutellarin (SC) on the rats with diabetic nephropathy (DN) and its molecular mechanism. Methods: Fifty rats were randomly allocated to five groups, ten rats each group, normal control group was fed with ordinary feed, DN model group, low, moderate and high doses groups of SC [25, 50 and 100 mg/(kg·d)] were given high fat feed, the rats were established by injecting streptozotocin (STZ) in four weeks, different dose groups of SC were drenched with SC after successfully modeling, DN model group accepted gastric lavage of physiological saline in the same volume, the rats were sacrificed in four weeks, the related factors were detected in the serum and renal tissue. Results: Compared with normal control group, the contents of BUN, Scr, glucose, insulin, HOMA-IR, MDA, TG, TC and LDL-C evaluated in the rats of DN model group (P＜0.05), different indexes lowered after treated by SC(P＜0.05), in dose-dependent manner; the contents of SOD, GSH, the levels of Nrf2/HO-1 mRNA and protein expressions lowered in DN model group, they decreased obviously after treated by SC(P＜0.05), in dose-dependent manner. Conclusion: SC could improve renal function of DN rats, decrease IR and lipidosis, and alleviate DN by regulating Nrf2/HO-1 signal channel and lightening oxidative stress reaction.

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备注/Memo

备注/Memo:
收稿日期：2017-05-04 *基金项目：湖北省自然科学基金项目(编号2015CFC187)。 作者简介：王玲(1982—)，女，主治医师。研究方向：糖尿病的基础医学研究。 △通讯作者：吴秋枫(1966—)，女，主任医师。研究方向：糖尿病的基础医学研究。
更新日期/Last Update: 2018-09-15