红芪黄酮气溶胶给药与灌胃给药对COPD大鼠的影响-《西部中医药》

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Title:: The Effects of Aerosal and Intragastric Administration of Astragalus Flavones for COPD Rats

文章编号:: 1004-6852(2019)06-0015-04

作者:: 蔺兴遥1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，3，4，张艳霞1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，李雪燕1，樊秦1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，张毅1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，马平川1，程晓丽1; 1 甘肃中医药大学，甘肃兰州 730000； 2 敦煌医学与转化省部共建教育部重点实验室； 3 甘肃省中药质量与标准研究重点实验室； 4 甘肃省中药药理与毒理学重点实验室

Author(s):: LIN Xingyao1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2，3，4, ZHANG Yanxia 1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, LI Xueyan 1, FAN Qin1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, ZHANG Yi1，2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2' target="_blank" rel="external">2, MA Pingchuan 1, CHENG Xiaoli 1; 1 Gansu University of Chinese Medicine, Lanzhou 730000, China; 2 Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and Ministerial Level; 3 Key Laboratory of Quality and Standard Research for Traditional Chinese Medicines of Gansu Province; 4 Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicines of Gansu Province (nurturing station)

关键词:: 小动物烟雾气溶胶染毒系统; COPD大鼠; 红芪黄酮; 气溶胶给药

Keywords:: animal smoke aerosal exposure system; COPD rats; astragalus flavone; aerosal administration

分类号:: R563

文献标志码:: A

摘要:: 目的：比较红芪黄酮灌胃给药及气溶胶给药两种不同的给药方法对大鼠慢性阻塞性肺病模型(COPD)的治疗作用。方法：选取40只SPF级雄性SD大鼠，随机分为空白对照组、模型对照组、红芪黄酮灌胃给药组及红芪黄酮气溶胶给药组，每组10只。空白对照组正常饮食喂养，不加任何处理措施。其余3组每天于小动物烟雾气溶胶染毒暴露系统吸入新鲜的香烟烟雾染毒造模，每天染毒30分钟，造模28天。造模后第7天开始给药，共21天。红芪黄酮灌胃给药组每日灌胃1 mL/100 g，37.41 mg/kg，给药，红芪黄酮气溶胶给药组气溶胶给药，气溶胶浓度保持在3.5 ～4.0 mg/m3，给药时间约40分钟。实验结束后，检测清醒及麻醉状态下各组动物肺功能，观察肺组织切片病理形态。结果：清醒状态及麻醉状态下造模组动物肺功能指标与空白对照组比较，均有显著性变化，差异有统计学意义(P＜0.05)；与模型对照组比较，各给药组显著改善肺功能相关指标，且气溶胶给药组作用更为显著。病理切片结果显示，与空白对照组比较，造模组出现不同程度的异常改变；与模型对照组比较，各给药组病理形态有显著改善，且气溶胶给药组作用更为显著。结论：气溶胶给药与灌胃给药相比，对COPD模型大鼠的治疗作用更为显著，气溶胶给药技术对于COPD的治疗具有重要意义。

Abstract:: Objective: To compare therapeutic effects of astragalus flavone in the treatment for COPD rats by aerosal and intragastric administration. Methods: Forty rats were chosen and randomized into normal control group, model control group, intragastric administration group and aerosal administration group, ten rats each group. Blank control group accepted normal diet and left unhandled. The rats in the other three groups were prepared into the models by inhaling fresh cigarette smoke in animal smoke exposure system, exposing for 30 minutes each day, for 28 days consecutively. The rat model were medicated since the seventh day after modelling, for 21 days. Intragastric administration group were drenched each day, 1 mL/100 g, 37.41 mg/kg, aerosal administration group were medicated by aerosal, the concentrations kept at 3.5~4.0 mg/m3, medicated for around 40 minutes. By the end of the experiment, to detect pulmonary function of the rats in different groups when the rats were awake and under the state of anesthesia, to observe pathological morphology of pulmonary tissue. Results: Compared with blank control group, the indexes of pulmonary function in the rats under the waking and anesthesia states of the modelling group showed significant changes, the difference showed statistical meaning(P＜0.05); compared with model control group, different medication groups significantly improve the related indexes of pulmonary function, and the group of aerosal administration indicated more notable effects. The results of pathological slices presented, compared with blank control group, the modelling group displayed abnormal changes to different extents; compared with model control group, pathological morphology of different medication groups improved apparently, and the effects of aerosal administration group the most notable. Conclusion: Compared with intragastric administration, aerosal administration shows more notable effects on COPD rat model, the technology of aerosal administration is significant for the therapy of COPD.

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

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