[1]杨晓露,陈文莉△,陈红宇,等.扶正抑瘤方醇提物对人肺癌A549细胞增殖和调亡的影响[J].西部中医药,2019,32(07):12-15.
 YANG Xiaolu,CHEN Wenli,CHEN Hongyu,et al.Influence of Alcohol Extract from Healthy Qi- enhancing Tumor-inhibiting Prescription on the Proliferation and Apoptosis of Human Pulmonary Cancer A549 Cell[J].Western Journal of Traditional Chinese Medicine,2019,32(07):12-15.
点击复制

扶正抑瘤方醇提物对人肺癌A549细胞增殖和调亡的影响
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年07期
页码:
12-15
栏目:
出版日期:
2019-07-15

文章信息/Info

Title:
Influence of Alcohol Extract from Healthy Qi- enhancing Tumor-inhibiting Prescription on the Proliferation and Apoptosis of Human Pulmonary Cancer A549 Cell
文章编号:
1004-6852(2019)07-0012-04
作者:
杨晓露陈文莉△陈红宇史勤丁明明夏云
上海市宝山区中西医结合医院,上海 201999
Author(s):
YANG Xiaolu, CHEN Wenli△, CHEN Hongyu, SHI Qin, DING Mingming, XIA Yun
Baoshan Branch of Shanghai Municipality Hospital of Integrative Medicine, Shanghai 201999, China
关键词:
人肺癌A549细胞增殖凋亡扶正抑瘤方醇提物
Keywords:
human pulmonary cancer A549 cells proliferation apoptosis alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription
分类号:
R285.5
文献标志码:
A
摘要:
目的:探讨扶正抑瘤方醇提物对人肺癌A549细胞增殖抑制和诱导调亡的作用。方法:将人肺癌A549细胞在体外加入不同浓度的扶正抑瘤方醇提物72小时后,采用CCK8法检测该方的增殖抑制作用,以Annexin V-EGFP标记法,通过流式细胞术检测该方对人肺癌A549细胞凋亡的影响。结果:扶正抑瘤方醇提物能抑制人肺癌A549细胞的增殖,与浓度相关,与空白对照组比较有明显差异(P<0.05);显微镜下可见到明显的凋亡细胞,流式细胞检测结果提示随着浓度的增加,细胞凋亡率增加。结论:扶正抑瘤方醇提物能显著抑制人肺癌A549细胞增殖,可能与诱导细胞凋亡有关。
Abstract:
Objective: To explore the effects of alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription on inhibiting the proliferation and inducing the apoptosis of human pulmonary cancer A549 cells. Methods: After adding different concentrations of alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription to human pulmonary cancer A549 cells in vitro for 72 hours, the inhibitory effects on the proliferation was detected using CCK8 method, the effects of the prescription on the apoptosis of human pulmonary cancer A549 cells were detected using flow cytometry. Results: Alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription could inhibit the proliferation of human pulmonary cancer A549 cells, there was the obvious difference compared with blank control group(P<0.05); the notable apoptotic cells could be seen under the microscope, the results of flow cytometry showed that cellular apoptosis rate upped as the concentrations increased. Conclusion: Alcohol extract from healthy Qi-enhancing tumor-inhibiting prescription could obviously inhibit the proliferation of human pulmonary cancer A549 cells, which might be related to inducing cellular apoptosis.

相似文献/References:

[1]张艳霞,景明△,蔺兴遥,等.藏药湿生扁蕾提取物对人结肠癌 SW480 细胞增殖和周期的影响[J].西部中医药,2014,27(09):50.
[2]王晓荣,李湧健△,程彬彬.薯蓣皂苷元抗肿瘤作用及其机制研究[J].西部中医药,2014,27(05):140.
 WANG Xiaorong,,et al.Study on Anti-tumor Effects and the Mechanism of Diosgenin[J].Western Journal of Traditional Chinese Medicine,2014,27(07):140.
[3]任虹,杨丽霞,吴丽丽,等.止消通脉宁干预TGF—β1诱导HK—2细胞增殖的研究[J].西部中医药,2012,25(08):10.
 REN Hong,YANG Li-xia,WU Li-li,et al.Intervention of ZhiXiao TongMaiNing on Proliferation of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β1[J].Western Journal of Traditional Chinese Medicine,2012,25(07):10.
[4]赵美林,黄萍△.桂皮醛对人舌癌Tca8113细胞体外增殖的影响*[J].西部中医药,2013,26(10):10.
 ZHAO Meilin,HUANG Ping.Effects of Cinnamic Aldehyde on Proliferation of Tongue Cancer Tca8113 Cells in Vitro[J].Western Journal of Traditional Chinese Medicine,2013,26(07):10.
[5]赵生华.苦参碱诱导宫颈癌Siha细胞凋亡的机制研究[J].西部中医药,2015,28(06):8.[doi:2015/6/16 0:00:00]
[6]宫姣,李艳,郝庆.泽泻醇-B-乙酸酯对结直肠癌HCT116细胞增殖和周期的影响[J].西部中医药,2017,30(03):22.
 GONG Jiao,LI Yan,HAO Qing.Effect of Alisol-B-acetate on Proliferation and Cycle of Colorectal Cancer Cell Line HCT116[J].Western Journal of Traditional Chinese Medicine,2017,30(07):22.
[7]聂珍贵,王世全,高春艳,等.两种萘醌类化合物对人结肠癌Caco-2细胞增殖的影响[J].西部中医药,2017,30(11):44.
 NIE Zhengui,WANG Shiquan,GAO Chunyan,et al.The Effects of Two Kinds of Naphthoquinones on Caco-2 Cells Proliferation of Human Colon Cancer[J].Western Journal of Traditional Chinese Medicine,2017,30(07):44.
[8]郭燕军.大蒜素对人舌鳞状细胞癌Tca8113细胞生长的影响[J].西部中医药,2019,32(02):25.
 GUO Yanjun.Influence of Allimin on Tca8113 Cellular Growth of Human Tongue Squamous Cell Carcinoma[J].Western Journal of Traditional Chinese Medicine,2019,32(07):25.
[9]熊刚,邹华,胡承莲,等.绿原酸基于PI3K/Akt信号通路对结肠癌细胞增殖和凋亡的调控作用[J].西部中医药,2020,33(05):71.[doi:10.12174/j.issn.1004-6852.2020.05.20]
 XIONG Gang,ZOU Hua,HU Chenglian,et al.Effects of Chlorogenic Acid on the Proliferation and Apoptosis of Colon Cancer Cells Mediated by P13k/Akt Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2020,33(07):71.[doi:10.12174/j.issn.1004-6852.2020.05.20]
[10]何敏,岳鹏莹,白宝平.苦参碱通过Pink1/Parkin途径抑制线粒体途径促进肝癌细胞凋亡的机制?[J].西部中医药,2021,34(11):50.[doi:10.12174/j.issn.2096-9600.2021.11.11]
 HE Min,YUE Pengying,BAI Baoping.Mechanism of Matrine Inhibiting Mitochondrial Pathway and Promoting Apoptosis of Hepatoma Cells through Pink1/Parkin Pathway[J].Western Journal of Traditional Chinese Medicine,2021,34(07):50.[doi:10.12174/j.issn.2096-9600.2021.11.11]

备注/Memo

备注/Memo:
收稿日期:2018-06-26*基金项目:上海市宝山区科学技术委员会科技创新专项资金项目(编号16-E-15)。作者简介:杨晓露(1986—),女,硕士学位,主管中药师。研究方向:恶性肿瘤的中医药防治。△通讯作者:陈文莉(1971—),女,硕士研究生导师,博士学位,副主任医师。研究方向:肿瘤疾病的中医诊治。
更新日期/Last Update: 2019-07-15